Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,555,047	G→C	V655L (GTC→CTC)	malT →	DNA‑binding transcriptional activator MalT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,555,047	0	G	C	100.0%	56.1 / NA	18	V655L (GTC→CTC)	malT	DNA‑binding transcriptional activator MalT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (7/11);  total (7/11)

CGTGCGCAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTTGCTGCTTAATCAA  >  NC_000913/3554810‑3555292                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      cgggcgcAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGAttt                                                                                                                                                                                                                                           <  2:126327/247‑1 (MQ=255)                                            aatcggcGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTTGCAATGTTGATTCAATGCTCGCTGGCCAGTGGTGATTTAGATAACGCACGTAGACAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCAAAACGCCTGGATCTATAACGCCAACAAACCCCGGGTGTATTACTGGAAAATGCCCGGCCATAAAGCCGCCGCTgaaatctgactg                                                                                                                                                                                               >  2:59742/7‑240 (MQ=255)                                               cGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGt                                                                                                                                                                                            <  1:27022/251‑1 (MQ=255)                                                    gcgcGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTTCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATAc                                                                                                                                                                                       <  2:62747/251‑1 (MQ=255)                                                           gCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTaaa                                                                                                                                                                                <  1:107782/251‑1 (MQ=255)                                                                    aaGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGttt                                                                                                                                                                       <  1:141909/251‑1 (MQ=255)                                                                          tggcgtcTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCGGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGTGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATTACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGaa                                                                                                                                                                  <  1:158166/247‑1 (MQ=255)                                                                                           caGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAgg                                                                                                                                                >  2:110252/1‑251 (MQ=255)                                                                                                           gCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCTTCTGGAAAACCTGCTGGGGAATGTCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACGTGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCTAACAACCACTTCCTGCAAGGTCAATGGCGCAACAt                                                                                                                                 <  2:90354/250‑1 (MQ=255)                                                                                                                                                                                        aaCCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTATACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCgg                                                     >  2:162250/1‑249 (MQ=255)                                                                                                                                                                                        aaCCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCCGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCgg                                                     >  2:81360/1‑249 (MQ=255)                                                                                                                                                                                                            atatCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGAtc                               >  2:203540/1‑251 (MQ=255)                                                                                                                                                                                                                          ggATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCt                  <  1:108734/250‑1 (MQ=255)                                                                                                                                                                                                                                  aaCGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAgccgcc                                                                                                                                                                                                              >  2:169001/1‑54 (MQ=255)                                                                                                                                                                                                                                  aaCGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAgccgcc                                                                                                                                                                                                              <  1:169001/54‑1 (MQ=255)                                                                                                                                                                                                                                   aCGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTTGCTGCtt        <  2:232586/251‑1 (MQ=255)                                                                                                                                                                                                                                        aacaaACTCCGGGAGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTTGCTGCTTAATc    >  2:226809/1‑250 (MQ=255)                                                                                                                                                                                                                                         acaaACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTTGCTGCTTAATCaa  <  2:205998/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      CGTGCGCAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTTGCTGCTTAATCAA  >  NC_000913/3554810‑3555292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑