Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	792,018	C→T	G13E (GGA→GAA)	galE ←	UDP‑glucose 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	792,018	0	C	T	100.0%	111.5 / NA	33	G13E (GGA→GAA)	galE	UDP‑glucose 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/19);  total (14/19)

TTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGATTGCGCTTTTTATCTGAAAAAAGACGCGGTTTCATGCCTGCATGCGTCGA  >  NC_000913/791783‑792260                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   ttGTACCGATTCGCCCTCGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTa                                                                                                                                                                                                                                      <  2:57175/250‑1 (MQ=255)            cGCCCACGGCTTTCAGCCCTGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACTTTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAAc                                                                                                                                                                                                                           <  2:7410/250‑1 (MQ=255)                  cGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAAct                                                                                                                                                                                                                    <  2:28108/251‑1 (MQ=255)                                 cGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTGCGTTACGAATATCGCCTTCAACAAACGGTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTcc                                                                                                                                                                                                     <  2:204994/251‑1 (MQ=255)                                       ggATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGACATCAAAGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAgg                                                                                                                                                                                               >  1:226371/1‑251 (MQ=255)                                          tCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCtt                                                                                                                                                                                            >  1:39639/1‑251 (MQ=255)                                                 gtCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAGCTCTCATAATTCGCTCCATTAGGCTTATGGTAt                                                                                                                                                                                     <  2:120795/251‑1 (MQ=255)                                                           tGATCGTGCCGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAAcc                                                                                                                                                                            >  2:189726/1‑250 (MQ=255)                                                                    aGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAAc                                                                                                                                                                  <  1:227017/251‑1 (MQ=255)                                                                          tcGGTCATCAACGCTTCGTTACGACTATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACaa                                                                                                                                                                >  2:175649/1‑247 (MQ=255)                                                                           cGGTCATCAACGCGTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTTCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAAATCGCTCCATTAGGCGTATGGTATGAAATAACCATAGCATAATAAAGATg                                                                                                                                                           <  2:161189/251‑1 (MQ=255)                                                                                  caACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGaaa                                                                                                                                                      >  2:51265/1‑249 (MQ=255)                                                                                                         cTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTgg                                                                                                                             >  1:199741/1‑251 (MQ=255)                                                                                                                                  gccTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCg                                                                                                    <  2:61710/251‑1 (MQ=255)                                                                                                                                         cGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTa                                                                                             <  1:175066/251‑1 (MQ=255)                                                                                                                                                  aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGAtt                                                                                    >  1:34864/1‑251 (MQ=255)                                                                                                                                                    aGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTaa                                                                                  <  1:182039/251‑1 (MQ=255)                                                                                                                                                         gTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGAt                                                                             <  2:176396/251‑1 (MQ=255)                                                                                                                                                             gCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGGATGACTTTCAATGTCACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATtat                                                                          >  2:123910/1‑250 (MQ=255)                                                                                                                                                                         gTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGGTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGGGATTATAAAACAGAGGGt                                                              <  2:42770/250‑1 (MQ=255)                                                                                                                                                                             cAGAGGTTATCAAGAATTATGACATCATGACCGTTGTGCAGTAATTGCACACAGGTATGTCTTTCAATGTAACCGCTACCCCCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAGAGATGCGAAAAGTTTGACATGGAATAAAGTATTGGAATCGTTTACACAAGAAGTTAGCCGGTTATGATGCGCGATGTAGTGATTATAAAACAGAGGGTTTatg                                                         <  1:213361/251‑1 (MQ=255)                                                                                                                                                                                    tATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGAtt                                                  >  2:124028/1‑251 (MQ=255)                                                                                                                                                                                     aTCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTCACCGCTACCACCGGTAACCAGAACTCTCATAATTAGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGTAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATCAAACAGCGGTTTTATGAATGAtt                                                  <  2:152297/250‑1 (MQ=255)                                                                                                                                                                                     aTCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGAtt                                                  <  1:28843/250‑1 (MQ=255)                                                                                                                                                                                     aTCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTAGAAAACAGTGGGTGTATGAATGAtt                                                  <  1:179061/250‑1 (MQ=255)                                                                                                                                                                                        aaGAATGATGACATCATGACCGTTTTGCAGTAATTGCACAAAGGTATGACTTTCAATGTAAACGCTACCACCGGTAAACAGAAATCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGATTgcgc                                              >  1:12122/1‑251 (MQ=255)                                                                                                                                                                                         aGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGATTGCGCt                                             <  2:49077/251‑1 (MQ=255)                                                                                                                                                                                          gAATGATGACATCATGACCGGTTTGCAGTAATTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGATTGCGCtt                                            <  2:88972/251‑1 (MQ=255)                                                                                                                                                                                                                    aGTAAGTGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACCTGGAATAAATTAGTGGAATCGTTTACACAAGACTTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGATTGAGCTTTT‑ATCTGAAAACAGACGCGGGTTCAt                 >  2:212123/1‑251 (MQ=255)                                                                                                                                                                                                                         ttGCACACAGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGATTGCGCTTTTTATCTGAAAAAAGACGCGGTTTCATGCCt             >  1:32666/1‑251 (MQ=255)                                                                                                                                                                                                                                caGGTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATAAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGATTGCGCTTTTTATCTGAAAAAAGACGCGGTTTCATGCCTGCATGCg      >  2:114934/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggTATGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGGGATTATAAAACAGAGGGTTTATGAATGATTGCGCTTTTTATCTGAAAAAAGACGCGGTTGCATGCCTGCATGCGTc    >  1:190390/1‑251 (MQ=255)                                                                                                                                                                                                                                     aTGACTTTCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGATTGCGCTTTTTATCTGAAAAAAGACGCGGTTTCATGCCTGCATGCGTCGa  <  1:101976/250‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   TTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATAATTCGCTCCATTAGGCTTATGGTATGAAATAACCATAGCATAACAAAGATGCGAAAAGTGTGACATGGAATAAATTAGTGGAATCGTTTACACAAGAATTTAGCCGTTTTTTATGCGCGATTAAGTGATTATAAAACAGAGGGTTTATGAATGATTGCGCTTTTTATCTGAAAAAAGACGCGGTTTCATGCCTGCATGCGTCGA  >  NC_000913/791783‑792260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑