Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,439,485	C→T	E9K (GAG→AAG)	yhdN ←	DUF1992 domain‑containing protein YhdN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,439,485	0	C	T	100.0%	38.1 / NA	15	E9K (GAG→AAG)	yhdN	DUF1992 domain‑containing protein YhdN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/10);  total (5/10)

GGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCA  >  NC_000913/3439268‑3439724                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                ggATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAAGTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGGTCGCCGCTTCCGGCCATGTTATCAAACTTACCTTTCGCTTGCTGTTCTGCGATATGGCGCGTTGCCCACTGGTCAAGTAACCACATAAATACTcc                                                                                                                                                                                                                <  1:20809/251‑1 (MQ=255)                        gagaATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACa                                                                                                                                                                                         <  1:12101/251‑1 (MQ=255)                                        tGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGCTGGCACGGGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGATCAGCTATTGTAGATAAGt                                                                                                                                                                         >  1:217496/1‑251 (MQ=255)                                                       cGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGt                                                                                                                                                          >  1:62618/1‑251 (MQ=255)                                                                                aaCCGGCATTCTTCAGCAAGCGTTACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATAAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTGGCGCTTCTGCTATATGGCGCTTTGCCCACTGTTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGTTATTGTAGATAAGTGGGGATACTAAGGGTATAAAAAAACCCGCCGGGGCGGGtt                                                                                                                                 <  2:111215/251‑1 (MQ=255)                                                                                                                   aaTTCCGGTGTCACGTGAGAATCATCATCCAGTATCATTGGTTCGCCGCTACCTGCCAGGTTATCAAACTCCCCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCtt                                                                                              <  2:183555/251‑1 (MQ=255)                                                                                                                                  ggagaAGCAGCATCCAGTATCAATGGTGAGCCGCGACCAGCCAGGTTATCAAACGCACCGTGCGCTTGCGCTTCTGATATATGGCGCGTTGCCCACTGGTCAAGTAACCAAAAAAATACTCCAGGGGAGGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATCTTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGAAGCAGCGTCTGCTTTATCTGAAc                                                                               <  2:79352/250‑1 (MQ=255)                                                                                                                                                    aTCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGGTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGt                                                             >  1:6354/1‑251 (MQ=255)                                                                                                                                                            ttCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATCTGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCAt                                                      <  2:185898/250‑1 (MQ=255)                                                                                                                                                                ccgcTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGGTTTTTTACTTTGCTTCCGATTACTCTGCCGCAGCTTCTGCTTTCTCTGAACGCTCAACCCGCTCGATGTAAACCAACGgcg                                                 >  2:92489/1‑251 (MQ=255)                                                                                                                                                                     cccTGCCAGGTTAACAAACTCCCCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTg                                            <  2:195588/250‑1 (MQ=255)                                                                                                                                                                      ccTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAAAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGt                                           <  2:65465/251‑1 (MQ=255)                                                                                                                                                                                   cAAACTCACCTTTCGCTTGCGCTTCTGCTATTTGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGa                               <  1:5614/250‑1 (MQ=255)                                                                                                                                                                                               tCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGAATAAAAAAACCCGCCGGGGCGGGGTTTTTTACGTTGCTTCAAATTAATCTGCAGCCGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATATAAGCCATCGGCGCGTTGTCGCCTGCAAGGAAGCCACACTTc                    >  1:109803/1‑249 (MQ=255)                                                                                                                                                                                                                atatGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAacca  <  2:98685/250‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                GGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCA  >  NC_000913/3439268‑3439724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑