Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,326,537 C→A S133S (TCG→TCT)  ftsH ← ATP‑dependent zinc metalloprotease FtsH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,326,5370CA96.3% 86.5 / NA 27S133S (TCG→TCT) ftsHATP‑dependent zinc metalloprotease FtsH
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (14/12);  total (15/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGAACTTTCGCTTCGCCTGCAATCGCTTTCGCCAGCAGCGTTTTACCGGTACCCGGAGGACCGACCATCAAGACGCCTTTCGGGATCTTACCGCCGAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTG  >  NC_000913/3326300‑3326783
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      
cGGAACTTTCGCTTCGCCTGCAATCGCTTTCGCCAGCAGCGTTTTACCGGTACCCGGAGGACCGACCATCAAGACGCCTTTCGGGATCTTACCGCCGAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCtt                                                                                                                                                                                                                                           <  1:170853/251‑1 (MQ=255)
    aCTTTCGCTTCGCCTGCAATCGCTTTCGCCAGCAGCGTTTTACCGGTACCCGGAGGACCGCCCATCAAGACGCCTTTCGGGATCTTACCGCCGAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGcc                                                                                                                                                                                                                                       <  1:162786/251‑1 (MQ=255)
            ttcgcCTGCAATCGCTTTCGCCAGCAGCGTTTTACCGGTACCCGGAGCAACGACCATAAAGACGCCTTTCGGGATATTACCGCCGAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATATGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACcgccgc                                                                                                                                                                                                                               >  1:100136/1‑251 (MQ=255)
                 cTGCAATCGCTTTCGCCAGCAGCGTTTTACCGGTACCCGGAGGACCGACCATCAAGACGCCTTTCGGGATCTTACCGCCGAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGc                                                                                                                                                                                                                          >  1:93994/1‑251 (MQ=255)
                        cGCTTTCGCCAGCAGCGTTTTACCGGTACCCGGAGGACCGACCATCAAGACGCCTTTCGGGATCTTACCGCCGAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGAc                                                                                                                                                                                                                   >  2:81029/1‑251 (MQ=255)
                                                   aCCCGGAGGACCGACCATCAAGACGCCTTTCGGGATCTTACCGCCGAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCa                                                                                                                                                                                        <  1:211036/251‑1 (MQ=255)
                                                                      aaGACGCCTTTCGGGATCTTACCGCCGAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCCACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGatcc                                                                                                                                                                      >  2:54829/1‑247 (MQ=255)
                                                                         aCGCCTTTCGGGATCTTACCGCCGAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGga                                                                                                                                                                  <  2:140808/251‑1 (MQ=255)
                                                                                           ccgcggaggttgtGGAGGCGGCTCGGCTCGCGCAGATACGCAACCAGTTAACCAGCGTCTGCGGTTGCTTCGTCGCAGCTCGCAACGTCAGCAAAGGTCTGTTTGATCTGAGCTGCCGTCAGCATGCGCTCTGTGCGCGGACCAAAAGACATGGCTCCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACTCTAATCAGCAACAGCAGCGGGAACCAGGAGATGAAGAGAGAAGAcag                                                                                                                                                <  1:36259/239‑1 (MQ=255)
                                                                                                                        gcgcAGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCACCGTCAGCAAAGGTAGTTTTAATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCg                                                                                                                    >  1:31207/1‑250 (MQ=255)
                                                                                                                           cAGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACa                                                                                                                 >  1:44431/1‑250 (MQ=255)
                                                                                                                            aGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAAc                                                                                                               >  2:105641/1‑251 (MQ=255)
                                                                                                                                     aCCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGtt                                                                                                      >  1:110919/1‑251 (MQ=255)
                                                                                                                                                aaCTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCaa                                                                                                                                                                                           >  2:18206/1‑155 (MQ=255)
                                                                                                                                                aaCTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTGCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTGTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCaa                                                                                                                                                                                           <  1:18206/155‑1 (MQ=255)
                                                                                                                                                         ttttGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATcc                                                                                   <  2:149677/250‑1 (MQ=255)
                                                                                                                                                                tcgtcgCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAAtt                                                                            <  1:129877/250‑1 (MQ=255)
                                                                                                                                                                    cgCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGa                                                                       <  1:836/251‑1 (MQ=255)
                                                                                                                                                                     gCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGAt                                                                      >  1:164732/1‑251 (MQ=255)
                                                                                                                                                                                    aGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGt                                                       <  1:207240/251‑1 (MQ=255)
                                                                                                                                                                                            tCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCGTTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGt                                                >  1:92280/1‑250 (MQ=255)
                                                                                                                                                                                                            ttCCGTCAGCATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTAtctt                               >  1:238927/1‑251 (MQ=255)
                                                                                                                                                                                                                     cATGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTaa                      <  1:176335/251‑1 (MQ=255)
                                                                                                                                                                                                                      aTGCGCGCTTTGCTCTTACCAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAAc                     >  1:99629/1‑251 (MQ=255)
                                                                                                                                                                                                                               tttctctTACCAAACGACATGGCACCTTTGCCACCGCCGCCCTGCATTTTACGCATGAAGAAGATCCAGACACCAATCCGCAACAGCATCGGGAACCAGGAGATGACGATAGCCGCCAGCAGGCTTGGTTCTTCAGGCGTTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTCAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTTATTTc            >  2:38403/4‑251 (MQ=255)
                                                                                                                                                                                                                                 gCTCTTACCAAAAGACATGGCACCTGTGCCACCGCCGCCATGCATTTGACGCATGAAGAAGAGCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGAGGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCGTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCAc          <  2:176748/251‑1 (MQ=255)
                                                                                                                                                                                                                                         cAAAAGACATGGCACCTTTGCCACCGCCGCCCTGCATGTGACGCATGAAGAAGAACCAGACACCAATAAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGGTCACCGATGACCTTCACGTTCTTGGTCAACAGGCTAACCAGTAAGCTCGGCTCCTGAACCGGAATGTAAGGGGCAGAACGGTTACTATCGTTATTGGTAACTTTGCGTTCACGGCCGttt  >  2:170730/1‑250 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      
CGGAACTTTCGCTTCGCCTGCAATCGCTTTCGCCAGCAGCGTTTTACCGGTACCCGGAGGACCGACCATCAAGACGCCTTTCGGGATCTTACCGCCGAGTTTCTGGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAGCAACTTCTTCTTTTGCTTCGTCGCAGCCCGCAACGTCAGCAAAGGTCGTTTTGATCTGATCTTCCGTCAGCATGCGCGCTTTGCTCTTACCAAACGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTG  >  NC_000913/3326300‑3326783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑