Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,208,513	G→T	G164W (GGG→TGG)	ttdT →	L‑tartrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,208,513	0	G	T	96.5%	90.1 / ‑3.4	29	G164W (GGG→TGG)	ttdT	L‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (16/12);  total (16/13)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01

AGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTT  >  NC_000913/3208269‑3208757                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     aGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGAtt                                                                                                                                                                                                                                                 >  2:101816/1‑250 (MQ=255)      ttAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATtatctat                                                                                                                                                                                                                                           >  2:33404/1‑251 (MQ=255)      ttAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATtatct                                                                                                                                                                                                                                             <  2:87722/249‑1 (MQ=255)        aaaTTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATtatctatc                                                                                                                                                                                                                                          <  2:82122/250‑1 (MQ=255)              aCTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTCATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGTCACAGGCTATGACAATACCGGGCTTGGACGCCTCATTGCTCTGATTCTGGTGAAAAAGCTGGGACATCGCACGCTGTTTCTCGGCTATGCTGTGATGTTCTTCTAGCTGTTCCTCGCACCTGTCACACCTTCCAACTCGCCGCGTGTTGCGTGGTTTCTCTATcccctctt                                                                                                                                                                                                                                   >  2:56363/1‑246 (MQ=255)                 gCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCatcatc                                                                                                                                                                                                                                  <  1:196937/249‑1 (MQ=255)                      aTCCCTCTCTTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGTTGCGTGGATTATCTATCCCATCATCCGTAAcc                                                                                                                                                                                                                           <  1:60350/251‑1 (MQ=255)                               gTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTACCCTGCCACCGc                                                                                                                                                                                                                  >  1:135213/1‑251 (MQ=255)                                     cGTTTCCGGTTTTTCTAATTCGGGTATCTGGCTGATTTACGCCGCCTTTATGTTTGGAACAGGCTATGAAAAAACCGGGCTTGGACGCCGAATTGCGCTGATTCTGATGAAAAAGATGGAACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGAACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGAAACTTGCCACACCTCTatc                                                                                                                                                                                                            >  2:216889/1‑251 (MQ=255)                                              tttttCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAAc                                                                                                                                                                                                   >  2:116034/1‑251 (MQ=255)                                                                      gATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAGCCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCgcagca                                                                                                                                                                           <  2:5654/251‑1 (MQ=255)                                                                      gATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCgcag                                                                                                                                                                             <  1:177473/249‑1 (MQ=255)                                                                           tCGCCTCCTTTATGTTTGTCACAGGCTATGAAAAACCCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAGCCTGCCGCCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTgg                                                                                                                                                                       <  2:212835/250‑1 (MQ=255)                                                                               cgccTTTATGTTTGGCACAGGCTATGAAAAAAACGGGCTTAGACGCCGCATTGCGCTGATTCTGGTGAAAAAGCTGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGCTGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGAAGTTCGCGCAGCATTGGCTCGt                                                                                                                                                                  >  1:134743/1‑251 (MQ=255)                                                                                                caGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCAGTGGCTCGTACATCATGTGGATgggg                                                                                                                                                 <  2:231033/251‑1 (MQ=255)                                                                                                    cTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGAACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGGACATCCTGTGGATagggtttt                                                                                                                                             >  2:215590/1‑247 (MQ=255)                                                                                                               ccGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGAGTGTTGCCGAcggg                                                                                                                                  >  2:239784/1‑248 (MQ=255)                                                                                                                  ggattGGACGCCGCATTGCCATGATTCTGGTGAAAAAGAAGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTATCCGAGCTGCTCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACATGCCACCGCTCTATCAATCACAACCAAACGACCGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTTTTGcggaacgcgtg                                                                                                                               >  2:152169/4‑241 (MQ=255)                                                                                                                             cgcATTGCGCTGATTCTGGTGAAAAAGCTGGGACATCGCACGCTGTTTCTCGGCTATACGGCGATGTTCTCCGAGCTGATCATCGCCCCTGTACCACCGTCCAACTCGGAGCGTGGTGCGTGGATTATCTATCCCATCATCCGTCACCTGCCACCGCTCTATCAATCACACCCAAACGACAGCCGTTCGCACAGAATTGGCTCGTACACCATGTGGATGGGGCTTGTTGCCGACTGCGTGTCCAGCGCCAt                                                                                                                    >  1:160138/1‑251 (MQ=255)                                                                                                                                       tGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACg                                                                                                          >  2:177265/1‑251 (MQ=255)                                                                                                                                        gATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGAc                                                                                                           <  2:237122/249‑1 (MQ=255)                                                                                                                                         aTTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCACTCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGc                                                                                                        >  1:100756/1‑251 (MQ=255)                                                                                                                                             tGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTCTGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCAACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGCTGGGGATTGTTGCCGACTGCGTGACCAGCGCCCTTTTCTTGACGGCGATg                                                                                                    >  2:136233/1‑251 (MQ=255)                                                                                                                                               gTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGGTTGGTGCCGACTGCGTGACCCGCGCCATTTTCTTGACGGCGATGGc                                                                                                  >  1:234563/1‑251 (MQ=255)                                                                                                                                                  aaaaaGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCAcc                                                                                               <  1:223459/251‑1 (MQ=255)                                                                                                                                                                  gCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAAtt                                                                               <  1:168687/251‑1 (MQ=255)                                                                                                                                                                                           gATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTcac                                                       <  1:208995/250‑1 (MQ=255)                                                                                                                                                                                                                         aaCACCGTCCAACTCGGCGCGTGGTGCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTc                         <  2:68262/250‑1 (MQ=255)                                                                                                                                                                                                                                               ggtgCGTGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGCCAGCAGTTCGCGCAGCATTGGCTCGTCCATCATGTGGATGGGGATTGTTGCCGACTGCGTGATCAGCGCCATTTTCTTTACGGCGATGGGACCCAACTTGCTGGTAATTGGGCTGGTGGAAAGCGCCTCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGTGGTTGCCGCTCAGCAtttt  >  2:2359/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     AGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTTGGACGCCGCATTGCGCTGATTCTGGTGAAAAAGATGGGACATCGCACGCTGTTTCTCGGCTATGCGGTGATGTTCTCCGAGCTGATCCTCGCACCTGTAACACCGTCCAACTCGGCGCGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCACCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTT  >  NC_000913/3208269‑3208757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑