Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,050,220	G→T	P150T (CCG→ACG)	gcvT ←	aminomethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,050,220	0	G	T	100.0%	82.9 / NA	23	P150T (CCG→ACG)	gcvT	aminomethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/14);  total (9/14)

GCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATAACCAGTGGTGGCAATAAACAGATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCA  >  NC_000913/3049986‑3050462                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   gCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGATTAACCGGTATAACCAGTGGTGGCAATAAACAGATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATc                                                                                                                                                                                                                                    >  2:111960/1‑251 (MQ=255)                  cccAAGCCACATGGCTTAACACCCGCTTCCCCCAGCGCACGCCAGAATGCGGCCGCTTTTTCATTGGGCACCGCAATTTCATAGCCCACTTCACCGGTAAAACCAGGGGTGGCAATAACCAGATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACg                                                                                                                                                                                                                   <  2:31068/251‑1 (MQ=255)                     aaGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATAACCAGTGGTGGCAATAAACAGATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAAc                                                                                                                                                                                                                <  1:74/251‑1 (MQ=255)                      aGCCACAGGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTAATTGGGCAGCGCAAGTTCATAGCCCGCTGCGCCGGTATAACAAGGGGTGGCAATAAACAGATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACg                                                                                                                                                                                                               <  1:38380/251‑1 (MQ=255)                                  tAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATAACCAGTGGTGGCAATAAACAGATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGAt                                                                                                                                                                                                    <  1:16971/250‑1 (MQ=255)                                           cTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATAACCAGTGGTGGCAATAAACAGATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGt                                                                                                                                                                                          >  1:75995/1‑251 (MQ=255)                                                cccaGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATAACCAGTGGTGGCAATAAACAGATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGc                                                                                                                                                                                     <  1:219824/250‑1 (MQ=255)                                                 ccaGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATAACCAGGGGTGGCAATAAACAAATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCg                                                                                                                                                                                    <  2:228853/251‑1 (MQ=255)                                                               aaaTCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATACCCATTGGTGGCAATAAACAGATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCa                                                                                                                                                                      <  1:23808/251‑1 (MQ=255)                                                                                      cAGCGCAATTTCATAGCCCGCTTCACCGGTATAACCAGTGGTGGCAATAAACAGATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTgg                                                                                                                                               >  1:147905/1‑251 (MQ=255)                                                                                             aTTTCCTAGCCCGCTTCACCGGTATAACCAGTGGAGGCAATAAACAGATCGCCCGCCTGGACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGACAGGTCATCACGAACGGAAATTTCGTTGCCGAAAGGTTCAGCGTGTTGGGAAATCCGGGAGCGGGCTTTTTCGCGGGGGGCGGAGtg                                                                                                                                        >  2:133012/1‑250 (MQ=255)                                                                                                 caaaGCCCGCTTCACCGGTATAACCAGTGGTGGCAATAAAAAGATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCGTTTTCGCGGGTGGCGGAGTTaac                                                                                                                                     <  2:44958/247‑1 (MQ=255)                                                                                                            tCACCGGTATAACCAGTGGTGGCAATAAACAGATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGga                                                                                                                          <  1:18410/250‑1 (MQ=255)                                                                                                                     taaaaagggggggCAATAAATAGCTCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTTACGCTGGGAGTCATTAAACAGTGTGGCAGCTTTTGCATGCGCATTCGTCCCTTGCACGGCAATCATGTAAATGTCATCACGAACGGTAATTTCGATGCCTAAAGGTTCAGCGTGTTGGGTAATCCAGGTGAGGTCTTTTTCGCGGGTGACGGAGTTAACAACGAGGCGGAAGAAATCTTc                                                                                                                >  1:178747/12‑251 (MQ=255)                                                                                                                                          aGATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAg                                                                                           <  1:128201/251‑1 (MQ=255)                                                                                                                                               gcccgccTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCAt                                                                                      >  2:66425/1‑251 (MQ=255)                                                                                                                                                                              ccTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCCGTAAAGTAGTAGACGATGAGGTCATCTATCCCACCGCCAGAGGCATTCAACATccc                                                       >  2:48379/1‑251 (MQ=255)                                                                                                                                                                                   cACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGt                                                  <  1:221270/251‑1 (MQ=255)                                                                                                                                                                                      cGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGATTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTaaa                                               <  2:237038/251‑1 (MQ=255)                                                                                                                                                                                                      tCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTTTACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAACGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCtttt                               >  2:185962/1‑251 (MQ=255)                                                                                                                                                                                                              cAGTGTGGCAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCt                       <  1:82347/251‑1 (MQ=255)                                                                                                                                                                                                                      cAGCTTTTGCCTGCGCATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCcac                >  1:3300/1‑250 (MQ=255)                                                                                                                                                                                                                                   cgcATTCGTCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCGTTTGGTGAGCTTCGCCACATCGTTCGCcagca  <  1:147000/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   GCAGCGTGTCACGCGCGCCCAAGCCACATGGCTTAACACCCGCTTCCACCAGCGCACGCCAGAAATCGGCCGCTTTTTCATTGGGCAGCGCAATTTCATAGCCCGCTTCACCGGTATAACCAGTGGTGGCAATAAACAGATCGCCCGCCTGCACGCCAAAGAACGGTTTCATCCCTTCCACCGCCTGACGCTGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCA  >  NC_000913/3049986‑3050462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑