Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,880,635	T→C	L138L (TTA→TTG)	casD ←	type I‑E CRISPR system Cascade subunit CasD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,880,635	0	T	C	100.0%	49.7 / NA	14	L138L (TTA→TTG)	casD	type I‑E CRISPR system Cascade subunit CasD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/8);  total (6/8)

ATACCATTCTCGGGAAGCAAATTGTCGAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCAAATTGCACACTCTCTGATA  >  NC_000913/2880407‑2880879                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     aTACCATTCTCGGGAAGCAAATTGTCGAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGACAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGTGGCTTCAATACTGCTTTTTCAAGTTCTg                                                                                                                                                                                                                                >  2:148634/1‑251 (MQ=255)   atcATTCTCGGGAAGCAAATTGTCGAGGCTAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTgc                                                                                                                                                                                                                               <  2:179851/248‑2 (MQ=255)                                                                           gCCCTGTAACTGATTCCGCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCTAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAGCCATCGTTGCATGGTGTGTTAACCAGAGAGCGACGGTAAAGGATGCATCACATAAATATTCgcgcta                                                                                                                                                       <  2:20751/250‑3 (MQ=255)                                                                                gTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAACAAAAGCGGGTGTGTTAGTGCGCAACTTCTCCGCCCCAGGCAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTCAAGGAGGCATCACATCAATATTCGCGCCATGTTTg                                                                                                                                                 >  1:221349/1‑251 (MQ=255)                                                                                                 atatatatCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACttt                                                                                                                                >  1:193686/1‑251 (MQ=255)                                                                                                     atatCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCaa                                                                                                                            <  1:62941/251‑1 (MQ=255)                                                                                                                                   aGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGAc                                                                                              <  1:81047/251‑1 (MQ=255)                                                                                                                                              aGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAAt                                                                                   <  2:186806/251‑1 (MQ=255)                                                                                                                                                gATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATc                                                                                  >  1:219134/1‑250 (MQ=255)                                                                                                                                                gATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCa                                                                                 <  2:3041/251‑1 (MQ=255)                                                                                                                                                          cTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGAGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATACATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATAACGCAACCCCg                                                                       >  2:71104/1‑251 (MQ=255)                                                                                                                                                                                     tgtTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGa                                             >  1:41783/1‑250 (MQ=255)                                                                                                                                                                                                                           aCCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCAAATTGCACActctct      <  2:192991/251‑1 (MQ=255)                                                                                                                                                                                                                               aGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACGCGACGATCGTCAAGAATGAGTTCAGCGCAGCGCACTGCAAATTGCACACTCTCTGATa  <  1:106180/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ATACCATTCTCGGGAAGCAAATTGTCGAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCAAATTGCACACTCTCTGATA  >  NC_000913/2880407‑2880879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑