Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,814,461	C→T	L91L (CTG→CTA)	luxS ←	S‑ribosylhomocysteine lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,814,461	0	C	T	100.0%	75.3 / NA	24	L91L (CTG→CTA)	luxS	S‑ribosylhomocysteine lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/15);  total (9/15)

TTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTAC‑GTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGG  >  NC_000913/2814230‑2814698                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              ttCCTGCAACTTATCTTTCGGCAGTGCCAGTTCTTCATTGATGTTGATCCG‑ACAGTCACGTTCCAACCTGCTACGCGCAATATCCTGCGCTTCCTGAAACGAGTGCATCTGGTAAGTGCCACAATGGTCGACGTTCAGTCCCGGGATCTGATTCTGATCCTACACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATaaacaacggt                                                                                                                                                                                                                           >  1:135455/1‑244 (MQ=255)   ccTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTAC‑GTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGAAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTg                                                                                                                                                                                                                          <  2:163849/250‑1 (MQ=255)            tctcTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTAC‑GTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGccc                                                                                                                                                                                                                 <  2:180506/250‑1 (MQ=255)                                    cGTTGCTGTTGATGCGTAC‑GTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATctct                                                                                                                                                                                         >  2:72935/1‑250 (MQ=255)                                               aTGCGTAC‑GGCACCTTCCAGAATGCTACACGCAATATCCTGCGCAGCCTGCAACGAGTTCATCTGGTAAGTGCCACCCTGTTAGACGTTCACTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAATACCGGTGCGGCAGCCCATTGGCGAGATTTCTATAATCTCTacccccttaacg                                                                                                                                                                             <  2:47043/251‑11 (MQ=255)                                                 gCGTAC‑GTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGt                                                                                                                                                                            <  2:195823/250‑1 (MQ=255)                                                         tCACGTTCCAGAATGCTACGCGCAATATCCTGCGCCTCCTCCAACGAGTCCATCTGGTACGTGCCACACTGGAAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATCTCGATAATCTCTACACCATTACCGTTAAGATgg                                                                                                                                                                    >  1:83335/1‑251 (MQ=255)                                                          cACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATACCGATAATCTCTACACCATTACCGTTAAGATGGt                                                                                                                                                                   >  1:7619/1‑251 (MQ=255)                                                            cGTTCCAGCATGACACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGCCGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGAAGCCCATTGGCGAGATACCGATAATCTCTACACCATTACCGTTAAGATGGtt                                                                                                                                                                  >  2:11840/1‑250 (MQ=255)                                                                              gcAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAAc                                                                                                                                               <  1:62514/251‑1 (MQ=255)                                                                                                        gTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGCTCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCctct                                                                                                                     >  1:56415/1‑251 (MQ=255)                                                                                                          gCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCtt                                                                                                                    <  1:72935/250‑1 (MQ=255)                                                                                                           cATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTGTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTTCCGTTAAGATGGGTACGCATAAAATCAGTAAACAGTTGCGCCGGGGGATGGATCCCTCTTTc                                                                                                                  <  2:97235/251‑1 (MQ=255)                                                                                                                ggTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTgg                                                                                                               <  1:12125/249‑1 (MQ=255)                                                                                                                 gTAAGTGCCACACTGGTAGACTTTCAGTTCCGGGATCTGATTCGGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTGCTGGCAt                                                                                                            <  2:151232/251‑1 (MQ=255)                                                                                                                       gCCACACTGGTAGACGTTCATTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCTCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACCCCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCActt                                                                                                       <  1:174618/250‑1 (MQ=255)                                                                                                                         cacaCTGGTAGACGTTCAGTTCCGGGATCTGCTTCTGATCCTGCACTTTCAGCACCTCTTCAATTGCCGCTTTCCCGGCATCAGCAACAAGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCCGCCCATTGGCGAGATATCGATAATCTCTATACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCActtctt                                                                                                    >  1:134011/1‑251 (MQ=255)                                                                                                                               ggTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCAGTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGtt                                                                                                <  2:80952/249‑1 (MQ=255)                                                                                                                                   gACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCAGTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCAc                                                                                          <  1:14163/251‑1 (MQ=255)                                                                                                                                   gACGTTCAGTTCCGGGATCTGATGCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCAc                                                                                          <  2:7333/251‑1 (MQ=255)                                                                                                                                                gggATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGa                                                                             <  1:141509/251‑1 (MQ=255)                                                                                                                                                                                           ccGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCAGATAAAAGCCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGATAAGATGGTTACGCATAAAAACAGCAAACAGGTGCTCCAGGGCATGGATACCGCTTTCTAGCATCAATTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGttt                                  >  1:112711/1‑251 (MQ=255)                                                                                                                                                                                                ttCCAGGCATCAGCAACACGCTGCTCCTCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCCTTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAACCAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGACCACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGcc                             >  2:146553/1‑251 (MQ=255)                                                                                                                                                                                                                              ggCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCgg  <  2:28579/248‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              TTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTAC‑GTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGG  >  NC_000913/2814230‑2814698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑