Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,741,296	C→A	intergenic (‑146/‑64)	aroF ← / → yfiL	3‑deoxy‑7‑phosphoheptulonate synthase, Tyr‑sensitive/DUF2799 domain‑containing lipoprotein YfiL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,741,296	0	C	A	100.0%	58.5 / NA	19	intergenic (‑146/‑64)	aroF/yfiL	3‑deoxy‑7‑phosphoheptulonate synthase, Tyr‑sensitive/DUF2799 domain‑containing lipoprotein YfiL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/11);  total (8/11)

GAAAAGCGGCCTTCAGTTGTTCCGGAGTCATTAAAACCTGTTCGTCGGTAATATGTACGTTATTCAGCGCGTCTTTTTGCATGATGGCGATCCTGTTTATGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACCTCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTTATACTCACGCGCCAACCTTGACCAGCACCGACTGGTATGATGTCGGTATGGAAGATGCGATATCGGGCAGCGCCATAAAAGATGACGATGCATTTAGCGATTCACAGGCGGATCGCGG  >  NC_000913/2741069‑2741541                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      gAAAAGCGGCCTTCAGTTGTTCCGGAGTCATTAAAACCTGTTCGTCGGTAATATGTACGTTATTCAGCGCGTCTTTTTGCATGATGGCGATCCTGTTTATGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACCTATGTATAGATAAATTTACACTccc                                                                                                                                                                                                                                <  1:22135/251‑1 (MQ=255)                      ccGGAGTCATTAAAACCTGTTCGTCGGTAATATGTACGTTATTCAGCGCGTCTTTTTGCATGATGGCGATCCTGTTTATGCTCGTTTGCGATATTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAAGCCATATTTCGGACATTTTTATTTACACAGGCAATTTAGTCGCGCTTGCAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATg                                                                                                                                                                                                            <  1:137607/250‑1 (MQ=255)                                        gTTCGTCGGTAATATGTACGTTATTCAGCGCGTCTTTTTGCATGATGGCGATCCTGTTTATGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGagaaa                                                                                                                                                                                           <  2:211059/249‑1 (MQ=255)                                                              aTTCAGCGCGTCTTTTTGCATGATGGCGATCCTGTTTATGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGcc                                                                                                                                                                   >  1:55408/1‑251 (MQ=255)                                                              aTTCAGCGCGTCTTTTTGCATGATGGCGATCCTGTTTATGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGGTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGCCGCGCTTTCAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGcc                                                                                                                                                                   <  1:236663/251‑1 (MQ=255)                                                                      cgTCTTTTTGCATGATGGCGATCCTGTTTATGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCttgtt                                                                                                                                                           <  1:26474/251‑1 (MQ=255)                                                                                               gTTTATGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACCTAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTc                                                                                                                                    >  2:160450/1‑249 (MQ=255)                                                                                                    tGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTg                                                                                                                              >  2:157266/1‑250 (MQ=255)                                                                                                                             aGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTTATACTCACGCGCCAACCTTg                                                                                                    >  2:78758/1‑251 (MQ=255)                                                                                                                                                                            tatTTCGCACCTTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTTATACTCACGCGCCAACCTTGACCAGCACCGACTGGTATGATGTCGGTATGGAAGATGCGATATCggg                                                     >  2:161142/1‑251 (MQ=255)                                                                                                                                                                                      aTTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTTATACTCACGCGCCAACCTTGACCAGCACCGACTGGTATGATGTCGGTATGGAAGATGCGATATCGGGCAGCGCCAt                                            >  1:75947/1‑250 (MQ=255)                                                                                                                                                                                         tttatttaCACAGGCAATTTAGTCGCGCTTTCAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTTATACTCACGCGCCAACCTTGACCAGCACCGACTGGTATGATGTCGGTATGGAAGATGCGATATCGGGCAGCGCCATaaa                                         >  1:131889/1‑250 (MQ=255)                                                                                                                                                                                           tagttaCACATGCAATTTAGTCGCGCTTTCAACCCTTACCTATGTATAGATAAATGTACACTCCCGTTGAAAACAATCCGCTATGCGTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGAGCCTTATGCTCACGCGCCAACCGTGACCAGCGCCGACTGGTATGATGTCGGTATTGAAGATGCGATATCGGGCAgggcggtaaaag                                       <  2:99155/247‑12 (MQ=255)                                                                                                                                                                                                acacAGGCAATTTAGTCGCGCTTTCAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTTATACTCACGCGCCAACCTTGACCAGCACCGACTGGTATGATGTCGGTATGGAAGATGCGATATCGGGCAGCGCCATAAAATATGACg                                 <  1:90124/251‑1 (MQ=255)                                                                                                                                                                                                   caGGCAATTTAGTCGCGCTTTCAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTTATACTCACGCGCCAACCTTGACCAGCACCGACTGGTATGATGTCGGTATGGAAGATGCGAGATCGGGCAGCGCCATAAAAGATGACGATg                              <  2:109619/251‑1 (MQ=255)                                                                                                                                                                                                                       tCAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTTATACTCACGCGCCAACCTTGACCAGCACCGACTGGTATGATGTCGGTATGGAAGATGCGATATCGGGCAGCGCCATAAAAGATGACGATGCATTTAGCGATTCACAGGc           <  2:55408/250‑1 (MQ=255)                                                                                                                                                                                                                        cAACCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTTATACTCACGCGCCAACCTTGACCAGCACCGACTGGTATGATGTCGGTATGGAAGATGCGATATCGGGCAGCGCCATAAAAGATGACGATGCATTTAGCGATTCACAGGCg          <  2:114380/250‑1 (MQ=255)                                                                                                                                                                                                                          aCCCTTACCTATGTATAGATAAATTTACACTCCCTTTGAACAAAATCCGCTACGCTTTCACAAAGGACAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCCTTACTGGTTAGCGGATGTCAGATTGCTCATTATACTAACGCGCCAACCTTGACCAGCACCGACTGGTATGATGTCGGTATGGAAGATGCGATATCGGGCAGAGCCATAAAAGATGACGATCCATTTAGCGATTCACAGGCGGAt       >  1:24406/1‑251 (MQ=255)                                                                                                                                                                                                                                aCCTATGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGTTTAGCGGATGTCAGATTGATCCTTATACTCACTCGCCAACCTTGACCAGCACCGACTGGTATGAGTTCTGTATTGAAGATTCGATATCGGGCAGCGCCATAAAAGATGACGATGCATTTAGCGATTCACAGGCGGATCGCgg  <  1:229028/250‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GAAAAGCGGCCTTCAGTTGTTCCGGAGTCATTAAAACCTGTTCGTCGGTAATATGTACGTTATTCAGCGCGTCTTTTTGCATGATGGCGATCCTGTTTATGCTCGTTTGCGATAGTTGATCCTCAGCGAGGATGACGTAACGATAACACATAAAGTAAAGTTTTCAATCCATATTTCGTACATTTTTATTTACACAGGCAATTTAGTCGCGCTTTCAACCCTTACCTCTGTATAGATAAATTTACACTCCCTTTGAAAACAATCCGCTATGCTTTGAAAAAGGAGAAAGAAATGATGAAAAAGTTTATCGCCCCCTTGTTGGCTTTACTGGTTAGCGGATGTCAGATTGATCCTTATACTCACGCGCCAACCTTGACCAGCACCGACTGGTATGATGTCGGTATGGAAGATGCGATATCGGGCAGCGCCATAAAAGATGACGATGCATTTAGCGATTCACAGGCGGATCGCGG  >  NC_000913/2741069‑2741541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑