Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,702,363	G→A	A36V (GCC→GTC)	recO ←	recombination mediator protein RecO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,702,363	0	G	A	100.0%	108.2 / NA	30	A36V (GCC→GTC)	recO	recombination mediator protein RecO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/15);  total (15/15)

AATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGGCAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTC  >  NC_000913/2702133‑2702597                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           aaTCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGccc                                                                                                                                                                                                                         <  1:166005/250‑1 (MQ=255)            aGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGt                                                                                                                                                                                                             >  1:124134/1‑251 (MQ=255)                cAGAGAAGCGCGTCTCGTATTCCAGTCCGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAg                                                                                                                                                                                                         <  2:4918/251‑1 (MQ=255)                    gaAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGt                                                                                                                                                                                                     >  2:181957/1‑251 (MQ=255)                             ctcGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTCCAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATc                                                                                                                                                                                             >  2:87265/1‑250 (MQ=255)                                                           gTTGATGTACAGACCGCTGTAAGGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCa                                                                                                                                                              <  2:16479/251‑1 (MQ=255)                                                                   aCAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGc                                                                                                                                                                       >  1:179383/1‑234 (MQ=255)                                                                   aCAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGc                                                                                                                                                                       <  2:179383/234‑1 (MQ=255)                                                                    cAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATg                                                                                                                                                     >  1:41808/1‑251 (MQ=255)                                                                       aCCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATgcgc                                                                                                                                                  <  1:88142/251‑1 (MQ=255)                                                                         cGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATgcgcgc                                                                                                                                                >  2:45670/1‑251 (MQ=255)                                                                                 aGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGcc                                                                                                                                        >  2:71216/1‑251 (MQ=255)                                                                                            gCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCATTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAg                                                                                                                             <  1:8023/251‑1 (MQ=255)                                                                                                     cAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCTTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTGCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCGCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGTCTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCTGAGTTACTCTTa                                                                                                                    <  2:234671/251‑1 (MQ=255)                                                                                                        cgcCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAg                                                                                                                 <  1:139822/251‑1 (MQ=255)                                                                                                          ccAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGCAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTaa                                                                                                                   >  2:90100/1‑247 (MQ=255)                                                                                                               gagaCGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCACGCCCAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGAGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGACCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTc                                                                                                           >  2:177009/1‑250 (MQ=255)                                                                                                                  aCGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACg                                                                                                       >  2:141075/1‑251 (MQ=255)                                                                                                                          agcccTGCGCAGCGTGTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCgag                                                                                               <  1:150169/247‑1 (MQ=255)                                                                                                                                      cGTTTTGCCTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTgc                                                                                   <  1:132015/251‑1 (MQ=255)                                                                                                                                         tttGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTgcgc                                                                                 <  2:28676/250‑1 (MQ=255)                                                                                                                                                          cgccAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAAc                                                               >  1:95926/1‑251 (MQ=255)                                                                                                                                                                             gggggTGAAAGGCTGTAATGCACCTGTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCAGCGGAGTTACTCTTAAAGATCGTCAACGTACCCGAGACTGCGCAGTGCGCGTTCGTCATCGGCCCAACCGGATTTCACTTTTAcacc                                            <  2:58308/250‑4 (MQ=255)                                                                                                                                                                                 gTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTTCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGAGTTCACTTGTACCCACAGt                                        <  2:100045/251‑1 (MQ=255)                                                                                                                                                                                            tAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGAt                                                                                                               >  2:209275/1‑169 (MQ=255)                                                                                                                                                                                            tAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTGAAAGAt                                                                                                               <  1:209275/169‑1 (MQ=255)                                                                                                                                                                                                  aCCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGgcgc                       >  1:96890/1‑251 (MQ=255)                                                                                                                                                                                                          gggTAGAGCGTTTAAAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAAc                >  2:228034/1‑250 (MQ=255)                                                                                                                                                                                                                 gCGTTTAGAGCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACGATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTGGTCGTCGGCCCAACCGGATTTCACTTTTACCCTCAGTTCAAGGTGAACAGGCGCTTCGAACAGTTCCTg        <  2:114446/251‑1 (MQ=255)                                                                                                                                                                                                                       agagCGTGCGCCTTTGACAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTc  >  1:205359/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           AATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGTGAAAGGCTGTAATGCACCTTTCAGGGTAGAGCGTTTAGAGCGTGCGCCTTTGGCAACCAGACGCACGCGCCCCGATTCCTCCGTGAAGACGTCCAGCATCAGGCTGGTTTCGCTCCACGGGCGACTATGCAGGACAAATGCGCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTC  >  NC_000913/2702133‑2702597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑