Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 471,115 G→A M160I (ATG→ATA mdlB → ABC transporter family protein MdlB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913471,1150GA96.3% 92.4 / NA 27M160I (ATG→ATAmdlBABC transporter family protein MdlB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/12);  minor base C (1/0);  total (15/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATTACGCGCAGTCGCTGCTGTTTAATCGGGCGGCAGTAGGCGTAGTGCAACAGTTGCGTACCGACGTGATGGATGCTGCGTTACGCCAGCCATTAAGCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATGTTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTCAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTA  >  NC_000913/470878‑471348
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                         
aCATTACGCGCAGTCGCTGCTGTTTAATCGGGCGGCAGTAGGCGTAGTGCAACAGTTGCGTACCGACGTGATGGATGCTGCGTTACGCCAGCCATTAAGCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGAc                                                                                                                                                                                                                               <  1:89123/250‑1 (MQ=255)
                        tAATCGGGCGGCAGTAGGCGTAGTGCAACAGTTGCGTACCGACGTGATGGATGCTGCGTTACGCCAGCCATTAAGCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATa                                                                                                                                                                                                       >  2:218705/1‑250 (MQ=255)
                            cGGGCGGCAGTAGGCGTAGTGCAACAGTTGCGTACCGACGTGATGGATGCTGCGTTACGCCAGCCATTAAGCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGGAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGAt                                                                                                                                                                                                  <  1:117415/251‑1 (MQ=255)
                             gggCGGCAGTAGGAGTAGTGCAACAGTTGCGTACCGACGTGATGGATGCTGAGTTACGCCAGCCATTAAGCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGAtt                                                                                                                                                                                                 >  2:160413/1‑251 (MQ=255)
                                   cAGTAGGCGTAGTGCAACAGTTGCGTACCGACGTGATGGATGCTGCGTTACGCCAGCCATTAAGCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCg                                                                                                                                                                                           <  2:182887/251‑1 (MQ=255)
                                       aGGCGTAGTGCAACAGTGGCGTACCGACGTGATGGATGCTGCGTTACGCCAGCCATTAAGCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCggtg                                                                                                                                                                                        <  2:186690/250‑1 (MQ=255)
                                                                  cGTGATGGATGCTGCGTTACGCCAGCCATTAAGCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGGAGTGGTGATATACCAGc                                                                                                                                                            >  2:240399/1‑251 (MQ=255)
                                                                     gatggatgCTGCGTTACGCCAGCCATTAAGCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGCGGTGCTGGTGGGAATGGTGGTATACCAGCGtt                                                                                                                                                         >  1:160356/1‑251 (MQ=255)
                                                                                cGTTACGCCAGCCATTAAGCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCg                                                                                                                                              >  1:8616/1‑251 (MQ=255)
                                                                                                aaGCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTgc                                                                                                                              >  2:99516/1‑251 (MQ=255)
                                                                                                 aGCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTgcg                                                                                                                             <  1:130725/251‑1 (MQ=255)
                                                                                                          gATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTCCCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTAttt                                                                                                                    <  2:175616/251‑1 (MQ=255)
                                                                                                                cAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCgg                                                                                                               >  1:151930/1‑250 (MQ=255)
                                                                                                                      gTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCa                                                                                                         >  2:206671/1‑250 (MQ=255)
                                                                                                                                        cgcgTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGAATTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCAACGACGGCTTTAACGAAat                                                                                      >  1:157192/1‑251 (MQ=255)
                                                                                                                                          cgTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCAACGACGGCTTAACGAAatcct                                                                                   >  2:107680/1‑249 (MQ=255)
                                                                                                                                                                  ccGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCcag                                                             <  1:101461/250‑1 (MQ=255)
                                                                                                                                                                      gATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATTAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGtt                                                        >  1:109946/1‑251 (MQ=255)
                                                                                                                                                                          tatACGTTAGCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGGGGGCGCGATGCTGGTGGCGATCTTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCCATGGCCTGCGCGTTATCCAGCAGTTTCGt                                                    >  2:39389/3‑251 (MQ=255)
                                                                                                                                                                           ctACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCg                                                     <  1:164320/249‑1 (MQ=255)
                                                                                                                                                                                 tACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTcagcag                                              <  1:135119/250‑1 (MQ=255)
                                                                                                                                                                                    cGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGTTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACAAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTACGCAGgcgc                                          >  1:152181/1‑251 (MQ=255)
                                                                                                                                                                                             aaCTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCCATGGCATGAGCGTTATCCAGCAGTTTCGTCAGCAGGCGCGATTTGGCg                                 >  2:206437/1‑251 (MQ=255)
                                                                                                                                                                                                                        gggCGCGATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTCAGCAGGCGCGATTTGGCGAACGTATTGGGGAGGCCAGTCGTTcac      <  2:8616/251‑1 (MQ=255)
                                                                                                                                                                                                                            gcgATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTCAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACta  <  2:178652/251‑1 (MQ=255)
                                                                                                                                                                                                                             cgATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCAACGACGGCTTTa                                                                                             >  1:106954/1‑159 (MQ=255)
                                                                                                                                                                                                                             cgATGCTGGTGGCGATATTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTGACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCAACGACGGCTTTa                                                                                             <  2:106954/159‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                         
ACATTACGCGCAGTCGCTGCTGTTTAATCGGGCGGCAGTAGGCGTAGTGCAACAGTTGCGTACCGACGTGATGGATGCTGCGTTACGCCAGCCATTAAGCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATGTTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTGGTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTGGCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTTCGTCAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTA  >  NC_000913/470878‑471348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: