Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,446,592	C→A	P294P (CCG→CCT)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,446,592	0	C	A	100.0%	49.3 / NA	19	P294P (CCG→CCT)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/8);  total (11/8)

TGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCGCATGGGCGATGTCAGCATCCAGGCGGTC  >  NC_000913/2446345‑2446804                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                     tGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGaggtaggt                                                                                                                                                                                                                   >  2:179394/1‑251 (MQ=255)    cAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTAGGTTTc                                                                                                                                                                                                                >  1:37746/1‑251 (MQ=255)     aaGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTAGGTTTc                                                                                                                                                                                                                >  1:75496/1‑250 (MQ=255)                   gcgcAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACTCAGGGATCGTGACGGCCTTTGGTGATCATCTCAACGTCTGCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTAGGTTTCAGCGCCATATGGGCa                                                                                                                                                                                                 <  1:231221/251‑1 (MQ=255)                     gcAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTAGGTTTCAGCGCCATATGGGCAAt                                                                                                                                                                                               <  1:56586/251‑1 (MQ=255)                                         tttttACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTAGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGctgctg                                                                                                                                                                            >  2:194853/1‑250 (MQ=255)                                                cAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAACCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGCCCCGGCACGGTAATGCTGGAGGTAGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATAccgcc                                                                                                                                                                    >  2:223226/1‑251 (MQ=255)                                                cAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGCAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTAGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATAccgcc                                                                                                                                                                    >  2:152731/1‑251 (MQ=255)                                                         atatCAGTCTTCACATCGGCATTTTGCGCCCGTTGCAGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTAGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATCCCGCCGAGAATgcc                                                                                                                                                           >  2:228699/1‑251 (MQ=255)                                                                           gCATTTTGCGCCAGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTAGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTg                                                                                                                                         >  1:72372/1‑251 (MQ=255)                                                                             aTTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTAGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCAGCTGCTGACACCGCCGAGAATGCCGCCCGCATGGTTGCTCTgg                                                                                                                                        >  1:179821/1‑250 (MQ=255)                                                                                     cccGTTGCCGTAACAGGTGATCCATTAAAACGATAGCCAGCATCGCTTCTGCCATCGGCACTGCGCGGATCCCGACACAGGGCTCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGCAGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCt                                                                                                                               >  1:149846/1‑251 (MQ=255)                                                                                                  cAGGTGATCCATTAACGCGAACGCCAGCATCTCTTCTGCTATCGGCACGGCGCGTATCCCGACACATGGTTCGTGACGGCCTTTGTTGATCACCTCAACTTCTTCTCCAAAGCTGTTAATGGTACGGCCCGGCACGGTAATTCTGGAGGTAGGTTTCAGCTCCATATGGGCAAGGATTTGCTGCGCGCTGCTGATACCGCCTAGATTGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCAt                                                                                                                  <  1:221790/251‑1 (MQ=255)                                                                                                       gATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTAGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCgg                                                                                                             <  1:194853/251‑1 (MQ=255)                                                                                                              tAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTAGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGc                                                                                                      >  2:88441/1‑251 (MQ=255)                                                                                                                                                     gcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTAGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCTAAttt                                                               <  1:49677/251‑1 (MQ=255)                                                                                                                                                                       aTCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTAGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGt                                             >  1:53939/1‑251 (MQ=255)                                                                                                                                                                          gTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTAGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGa                                          <  1:226490/251‑1 (MQ=255)                                                                                                                                                                                                         tcGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCGGGAGGTAGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCGCATGGGCGATGTCAGCa             <  2:194229/249‑1 (MQ=255)                                                                                                                                                                                                                  cGGTTAATGGTACGCCCCGGCACGGTAATGCTGGAGGTAGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCGCATGGGCGATGTCAGCATCCAGGCGGTc  <  2:224487/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                     TGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCGCATGGGCGATGTCAGCATCCAGGCGGTC  >  NC_000913/2446345‑2446804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑