Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,307,733 C→T A295T (GCG→ACG)  yojI ← ABC transporter family protein/microcin J25 efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,307,7330CT91.7% 52.0 / ‑5.0 24A295T (GCG→ACG) yojIABC transporter family protein/microcin J25 efflux protein
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (9/13);  total (9/15)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCTTGCGCATCAGGAA  >  NC_000913/2307504‑2307972
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               
cccGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGt                                                                                                                                                                                                                            <  1:168173/251‑1 (MQ=255)
     caacaaCACCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGGTCAGCTTGTTAAACGTCCCCTGCGCCGTCAGCAGCGTCGGc                                                                                                                                                                                                                        <  2:106932/250‑1 (MQ=255)
                                gCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCTTTTGCCAGTTGGGAAACGCCGGCGGGCGCGGAAACTCTGCTTGGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTAAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAgcggcg                                                                                                                                                                                            <  2:65729/251‑1 (MQ=255)
                                  tACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGt                                                                                                                                                                                           >  1:116135/1‑250 (MQ=255)
                                        ggccgccAAACAGAAACAGCAGCTCGCCACGTTTGATGGGGAGATTAATCGCACCAACGGAAAACGCGTGATCCTGATAAGCAACCTCCACGTTACGCAGCTCCAGCGTTTTCCGGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTGGTAAACCGTCCCCTGCGCCGTCAGCAGCGTCGGCAATGCGCAAACCGCCGAAAGCAGCGGCGTACGCAgg                                                                                                                                                                                    <  2:184197/250‑1 (MQ=255)
                                             ccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATa                                                                                                                                                                                >  1:177719/1‑250 (MQ=255)
                                                                                       aTCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGccc                                                                                                                                      >  1:213781/1‑250 (MQ=255)
                                                                                                 cGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTg                                                                                                                           <  1:55492/251‑1 (MQ=255)
                                                                                                  ggAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGt                                                                                                                          >  2:19699/1‑251 (MQ=255)
                                                                                                   gAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGtt                                                                                                                         >  1:37398/1‑251 (MQ=255)
                                                                                                      aaCGCGTTATCCGGATAAGAAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTGGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCCCCTGCGCCGTCAGCAGCGTCGGCAAGGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGGCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGc                                                                                                                      <  1:38802/251‑1 (MQ=255)
                                                                                                          cgTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATc                                                                                                                  <  1:88386/251‑1 (MQ=255)
                                                                                                           gTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCCTcc                                                                                                                 >  1:76746/1‑251 (MQ=255)
                                                                                                                            ccgtccggtgaCGCAGCTCCGGCGTTTGCCCGTGGGGAACCGCCTGCGGGCGCGGAAACTCTGCTTAAAAAGGCGCGAGCGCGAATTTGTCCAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAAGGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTGGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGAtt                                                                                                <  2:200535/241‑1 (MQ=255)
                                                                                                                            acgccccGTTACGAAGCTCCAGCGTTTGCAAGTGGGGAACCGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGGGGGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGAtt                                                                                                <  2:71335/245‑1 (MQ=255)
                                                                                                                                cacgtccctcagccccacggTTTGCCATTTGGGAACCGCTCGCGGGCGCGAAAACTCTGCATTAACAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTCAGCAGCGTCGGCAACGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACCCCAGGCCGATTgcgc                                                                                            <  2:1264/232‑1 (MQ=255)
                                                                                                                                cACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTgcgc                                                                                            >  2:232735/1‑251 (MQ=255)
                                                                                                                                                   gTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGACACTCTGCTTTGACAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTCCGCAGGCATAAAAGCGTCAACGAATAGGGCGCGGCAACGTTGGCATCAGCCCAACCGACGCTGTTCGCCATCCAGAACACCACGCCGATTGCGCCCAGCATCATGATGTTTg                                                                          >  1:164895/1‑250 (MQ=255)
                                                                                                                                                               ggAAAGGCCTGCGGGCGCGGAAACTCTGCTTTGAAGGTCGCGAGGGCGAATTTGTTCAGCTTGTTAAACGTCCCCTGCGCCTTCAGCAGCGTCGGCATTGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGc                                                             <  1:229013/251‑1 (MQ=255)
                                                                                                                                                                  aaccccTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGATTTTGTTCAGCTTGTTAAACGTCACCGGCGCCGTCAGCAGCGTCGGCAAGGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACt                                                          <  1:210507/247‑1 (MQ=255)
                                                                                                                                                                                  gAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTAAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCt                                          >  1:213596/1‑251 (MQ=255)
                                                                                                                                                                                  aaaaCTGTGCTTTGAAAGGCGCGAGAGCTAATTTGTCCAGCTTGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAAGGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCt                                          <  2:179927/250‑1 (MQ=255)
                                                                                                                                                                                                              aaaTTTGTTCAGCTTGTTAAACGCCACATGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCtt              <  1:63855/250‑1 (MQ=255)
                                                                                                                                                                                                                            tGTTAAACGTCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGAGACTCTTGCGCATCAGGaa  <  1:75885/249‑1 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               
CCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTCAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCTTGCGCATCAGGAA  >  NC_000913/2307504‑2307972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑