Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,247,134	A→C	Y467D (TAC→GAC)	lysP ←	lysine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,247,134	0	A	C	100.0%	73.1 / NA	24	Y467D (TAC→GAC)	lysP	lysine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/7);  total (17/7)

GCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATGG  >  NC_000913/2246917‑2247364                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       gCCTTTTGTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATAcc                                                                                                                                                                                                       <  2:175232/251‑1 (MQ=255)              gcggcgTAACGATGATGCAAACGATAACAATTAACAAGCCAAATGTTAAATTTTATATCCGCATGATTTGGCAGGGATATGGAATTGAAAAAGCCCGCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTGATTTCTTATCGTTCTGCGGGAACGTCAGGTCGCTGTAGCGTATGAAGAGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATGATCAGGAACAGCTTGATACCATTATACATTGcc                                                                                                                                                                                          <  2:134738/251‑1 (MQ=255)              gcggcgTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAAGCAGGAACAGCGGGATACCAATATACGTTGcc                                                                                                                                                                                          <  2:38938/251‑1 (MQ=255)                       cGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTAcgccg                                                                                                                                                                                 >  2:140681/1‑251 (MQ=255)                                 aaCGATAACAATTATCAATCCACATGATCACTTTTATATCCGCATGATTTGTCAGGGCAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTCGCGTACGAAGAGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCa                                                                                                                                                                        >  2:145976/1‑250 (MQ=255)                                            ttATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTc                                                                                                                                                            >  2:186716/1‑251 (MQ=255)                                                   tCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACg                                                                                                                                                     >  2:24274/1‑251 (MQ=255)                                                          gTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTAGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATACTCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGt                                                                                                                                               >  1:148069/1‑250 (MQ=255)                                                                ttttATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTg                                                                                                                                        >  1:42709/1‑251 (MQ=255)                                                                   tataTCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGc                                                                                                                                      <  1:61730/250‑1 (MQ=255)                                                                              gATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATaa                                                                                                                          <  2:104579/251‑1 (MQ=255)                                                                                   gTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATc                                                                                                                        >  1:41481/1‑248 (MQ=255)                                                                                         gAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGacac                                                                                                                  >  1:182094/1‑248 (MQ=255)                                                                                                             ctctCGGTTGAGAGGGCTTAGCAAGGACGGGAGGAAACTTATTTCTTATCGCACTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGAGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCggacc                                                                                           >  2:235522/1‑248 (MQ=255)                                                                                                                tcGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAACCTTATTTCTTATAGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGTCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGcccc                                                                                          >  2:223891/1‑249 (MQ=255)                                                                                                                                     ggaaggGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAATCCTGAACGGTa                                                                   <  1:63469/251‑1 (MQ=255)                                                                                                                                               aaaCTTCTTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGATTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCACAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGCAACCTGAACGGTACGGc                                                               >  2:224652/1‑245 (MQ=255)                                                                                                                                                                  cTGCGGGCAATTCATTTCGCTGTAGCGTACGCAGTGAGTTCCTTTAATCAGCTTGTCGCCGCACCAAATAATCAGGAACAGCGAGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAACGTGATACTCAGACACAGACTGAATGCGAAGATCGGCCCCAGTGGGAAGAACCCTGAACGGTACGGCAGATAGTTAACGTCGTGTCCCTGc                                       >  2:219545/1‑250 (MQ=255)                                                                                                                                                                      gggAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATAc                                  >  2:48359/1‑251 (MQ=255)                                                                                                                                                                         aaCTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGt                                >  1:235285/1‑250 (MQ=255)                                                                                                                                                                             tCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACcg                           >  1:116891/1‑251 (MQ=255)                                                                                                                                                                              cATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTACTGTCGTGTCCCTGCCATACGTAAcagc                          >  2:51881/1‑248 (MQ=255)                                                                                                                                                                                              aCGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTg          <  2:164418/251‑1 (MQ=255)                                                                                                                                                                                                      ggTTCCATTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCCACGACGCACCAGTCACTAGTATCTTTCAGGAACGCTTCGTAGTTCAGGCCCAAAGTGATAATCAGCCACAGAAAGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTCCGGCAGATCGTTAATACCGTGTCCATGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATgg  >  2:161504/2‑251 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       GCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATGG  >  NC_000913/2246917‑2247364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑