Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,830,6970GC53.3% 5.3 / 19.5 15G23G (GGC→GGG) astAarginine N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/7);  new base C (8/0);  total (8/7)
Fisher's exact test for biased strand distribution p-value = 1.55e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TAGACATTCAGCTCTTTTGAGGCGTGAACCAACGTGCCGACGCGATAGTTGTACCAGGGATCGTTCAGCCCAACCGCCACCTCAATGGCACAAATCCCCGCCACGGTGCCTGTCTCGCTATCTTCCAGCACGAACACATAGCCCTGCTCACTTTTGGGCAGTTCGCCTTGCCAGGTTTTGATTGCCCTTTCGATACGCGCCGAAAGCGTGGCTTCATTGGCGGG‑AAGCGACGTC‑AG‑GCCGCCGCCC‑GTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTT‑CGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAA  >  NC_000913/1830455‑1830883
                                                                                                                                                                                                                                                     |                                                                                                                                                                                            
taGACATTCAGCTCTTTTGAGGCGTGAACCAACGTGCCGACGCGATAGTTGTACCAGGGATCGTTCAGCCCAACCGCCACCTCAATGGCACAAATCCCCGCCACGGTGCCTGTCTCGCTATCTTCCAGCACGAACACATAGCCCTGCTCACTTTTGGGCAGTTCGCCTTGCCAGGTTTTGATTGCCCTTTCGATACGCGCCGAAAGCGTGGCTTCATTGGCGGGGAAG‑GACGTC‑AG‑GCCGCCCCCC‑Ctttt                                                                                                                                                                                     >  1:237916/1‑251 (MQ=255)
        cAGCTCTTTTGAGGCGTGAACCAACGTGCCGACGCGATAGTTGTACCAGGGATCGTTCAGCCCAACCGCCACCTCAATGGCACAAATCCCCGCCACGGTGCCTGTCTCGCTATCTTCCAGCACGAACACATAGCCCTGCTCACTTTTGGGCAGTTCGCCTTGCCAGGTTTTGATTGCCCTTTCGATACGCGCCGAAAGCGTGGCTTCATTGGCGGG‑AAGCGACGTC‑AG‑GCCGCCGCCC‑GTTTTGCTGGCa                                                                                                                                                                              <  1:166450/250‑1 (MQ=255)
                                            aTAGTTGTACCAGGGATCGTTCAGCCCAACCGCCACCTCAATGGCACAAATCCCCGCCACGGTGCCTGTCTCGCTATCTTCCAGCACGAACACATAGCCCTGCTCACTTTTGGGCAGTTCGCCTTGCCAGGTTTTGATTGCCCTTTCGATACGCGCCGAAAGCGTGGCTTCATTGGCGGG‑AAAGGAAGTC‑CA‑GCCGCCCCCC‑GTTTTGCTGGGAAACTGCATCAGCGCCCAAACAACTGAACGCTCaaagg                                                                                                                                         >  1:191849/1‑248 (MQ=255)
                                                    cccAGGGTTCTTTCAGCCCAACCGCCACCTCAAGGGCACAAATCCCCGCCACGGTGCCTGTCTCGCTATCTTCCAGCACGAACACAGAGCCCTGCTCACTTGTGGGCAGTTCGCCTTGCCAGGTTTTGATTGCCCTTTCGATACGCGGCGAAAGCGTGGCTTCATTGGCGGG‑AAGCGACGTC‑TG‑GCCGCCGCCC‑GTTTTGCTGTCAAGCTTCAGCTGCGCCGAGACATCTGATCGCTCAACGGGACGGATg                                                                                                                                 <  1:135442/250‑1 (MQ=255)
                                                                        aCCGCCACCTCAATGGCACAAATCCACGCCACGGTGCCTGTCTCGCTATCTTCCAGCACGAACACATAGCCCTGCTCACTTTTGGGCAGTTCGCCTTGCAAGGTTTTGATTGCCCTTTCGATACGCGCCGAAAGCGTGGCTTCATTGGCGGG‑AAACGACGGC‑CG‑GCCGCCCCCC‑GTTTTTGTCGCAAGCTGGACTAGCGCCGACACCTCTCAACGCTCAACAGGACgggagacacaccttgttgacgtcgg                                                                                                             >  2:232112/1‑228 (MQ=255)
                                                                             cACCTCAATGGCACAAATCCCCGCCACGGTGCCTGTCTCGCTATCTTCCAGCACGAACACATAGCCCTGCTCACTTTTGGGCAGTTCGCCTTGCCAGGTTTTGATTGCACTTTCGATACGCGCCGAAAGCGTGGCTTCATTGGCGGG‑AAGCGACGTC‑AA‑GCCGCCCCCCCGTTTT‑CTGGCAAGCCGGCTCACCGCCGAGACAACCGGTCGCCCCACGGGACGGGTGACCCTCATTATTAAACTCTGCTaa                                                                                                         >  2:18954/1‑250 (MQ=255)
                                                                                           aaaTCCCCGCCACGGTGCCTGTCTCGCTATCTTCCAGCACGAACACATAGCCCTGCTCACTTTTGGGCAGTTCGCCTTGCCAGGTTTTGATTGCCCTTTCGATACGCGCCGAAAGCGTGGCTTCATTGGCGGG‑AAGCGACGTC‑AG‑GCCGCCGCCC‑GTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAag                                                                                          <  1:223887/251‑1 (MQ=255)
                                                                                                   gCCACGGTGCCTGTCTCGCTATCTTCCAGCACGAACACATAGCCCTGCTCACTTTTGGGCAGTTCGCCTTGCCAGGTTTTGATTGCCCTTTCGATACGCGCCGAAAGCGTGGCTTCATTGGCGGG‑AAGCGACGTC‑AG‑GCCGCCGCCC‑GTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCa                                                                                  <  1:174566/251‑1 (MQ=255)
                                                                                                                     cTATCTTCCAGCACGAACACATAGCCCTGCTCACTTTTGGGCAGTTCGCCTTGCCAGGTTTTGATTGCCCTTTCGATACGCGCCGAAAGCGTGGCTTCATTGGCGGG‑AACCGACGTC‑AA‑GCCGCCCCCC‑CTTTTTTTTTCCATCTCCCTCCGCGCCGCGACATCTGATCCCTCCACGGGGCGGATGACACTAATGATGAACCTCGGCTAACAACGTGTTCGCAAGCAGCTGCACAGCGATCCAGTCCGgtc                                                                >  2:16196/1‑251 (MQ=255)
                                                                                                                                           aGCCCTGCTCACTTTTGGGCAGTTCGCCTTGCCAGGTTTTGATTGCCCTTTCGATACGCGCCGAAAGCGTGGCTTCATTGGCGGG‑AAGCGACGTC‑CG‑GCCGCCCCCC‑GTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGAACATCATGATGAACCTCGGCCAACAAAGGGTTCGCAAGCAGCTGCAAAGCGATCCCGCCCGGGCGTCACCCCTTCTT‑CGCTGACAt                                         >  1:65333/1‑251 (MQ=255)
                                                                                                                                           aGCCCTGCTCAATTTTGGGCAGTTCGCCTTGCCAGGTTTTGATTGCCCTTTAGATACGCGCCGAAAGCGTGGCTTCATTGGCGGG‑GAACGGAGTCCAGCGCC‑CACCCC‑GTTTTG‑TGGTAAACTGCATCAACGCAGAGAAATCTCATCGCTCCACAGGGCGGCTGAGCCTCAAGATGAACCCCAGCTAAAAAAGAGTTCGCAACAAGCTGCAAAGCCATCCATTACGATCGTCAACCCTTCTT‑CGCTGaaa                                          >  1:152530/1‑248 (MQ=255)
                                                                                                                                                 gcgcgctgtcgggCAGTTAGGCTTGCCAGAGTTAGATGGCCCTCTCGATTCGCGCCGACAGCGTGGCTTCGTGGGCGGG‑AAGAGAAGTC‑AG‑GCCGCCGCCC‑GTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCATCGGGACGTATGGCCATCATGATGAGCCTCGGCTAACAAATTGTTCGCAAGCAGCTGCAAGGCGATCCAGTGCGGTCGTCGCCTCTTCAT‑CGCTGACATTGAgcg                                   <  2:225520/241‑1 (MQ=255)
                                                                                                                                                          tGGGCAGTTCGCCTTGCCAGGTTTTGATTGCCCTTTCGATACGCGCCGAAAGCGTGGCTTCATTGGCGGG‑AAGCGACGTC‑AG‑GCCGCCGCCC‑GTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGACCCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTT‑CGCTGACATTGAGCGCAGGCGCa                           <  1:228558/250‑1 (MQ=255)
                                                                                                                                                                        tGCCAGGTTTTGATTGCCCGTTCGATACGCGCCGAAAGCGTGGCTGCATTGGCGGG‑AAGCGACGTC‑AG‑GCCGCCGCCC‑GTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTT‑CGCTGACATTGAGCGCAGGCGCAAAACGCACCACGt              <  2:83616/249‑1 (MQ=255)
                                                                                                                                                                                   gATTGCCCTTTCGATACGCGCCGAAAGCGTGGCTTCATTGGCGGG‑AAGCGGCGTC‑AG‑GCCGCCCCCC‑GTTTTGCTGGCAAACTGTATCCGCGCCGAGACATCTGATCTCTCAACGGGGCGGATGAACATCCTTATGAACCTCGGCTAACAAAGTGATCGCAAGCAACTGACAAGAGAAACAGACCGGTCGTCACCTCTTCTTTCCCT‑ACATTTAGGGAACGCGCAAAAACCACCACGTTGCCACCCGcca  >  1:232143/1‑248 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                            
TAGACATTCAGCTCTTTTGAGGCGTGAACCAACGTGCCGACGCGATAGTTGTACCAGGGATCGTTCAGCCCAACCGCCACCTCAATGGCACAAATCCCCGCCACGGTGCCTGTCTCGCTATCTTCCAGCACGAACACATAGCCCTGCTCACTTTTGGGCAGTTCGCCTTGCCAGGTTTTGATTGCCCTTTCGATACGCGCCGAAAGCGTGGCTTCATTGGCGGG‑AAGCGACGTC‑AG‑GCCGCCGCCC‑GTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTT‑CGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAA  >  NC_000913/1830455‑1830883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑