Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,042,111	IS5 (–) +4 bp	intergenic (+86/‑254)	zinT → / → yodB	metal‑binding protein ZinT/putative cytochrome b561 YodB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2042111 =	0 (0.000)	19 (0.800)	16/496	0.6	100%	intergenic (+86/‑257)	zinT/yodB	metal‑binding protein ZinT/putative cytochrome b561 YodB
	?	NC_000913	= 2102943	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region
*	?	NC_000913	= 2042114	0 (0.000)	20 (0.850)	19/496	0.4	100%	intergenic (+89/‑254)	zinT/yodB	metal‑binding protein ZinT/putative cytochrome b561 YodB
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region

TCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGC  >  NC_000913/2101520‑2101988                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                tCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGCAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGAGCGAATAAGCgggg                                                                                                                                                                                                                             >  1:119084/1‑250 (MQ=255) tCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACAAGCAAGGCTACGCCTCACGCACAGGCAAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCgggg                                                                                                                                                                                                                             >  1:99006/1‑250 (MQ=255)                                       aTAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCAt                                                                                                                                                                                      >  1:128827/1‑251 (MQ=255)                                          aTGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGtt                                                                                                                                                                                   >  1:132658/1‑251 (MQ=255)                                                         agccagGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAACGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGCTTTTTtc                                                                                                                                                                    >  2:218817/1‑251 (MQ=255)                                                              ggCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCg                                                                                                                                                               >  1:164934/1‑251 (MQ=255)                                                               gCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAAGGATCCTGCGCACCAATCAACAACCGTATCAGAAGAGATACTTTCGTTAGGAATTTTTGTTTTATTAAGGAATGGGCGAAGATGCGTGTGAATTCTGCTCGGCTGACTCAGTCGTTGCATTTCTTGATGGTTGGGCCTATTTTTCCGCCCGt                                                                                                                                                              <  2:216997/251‑1 (MQ=255)                                                                    gCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTAAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCAGTAAATg                                                                                                                                                         >  1:20727/1‑251 (MQ=255)                                                                                                gTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGTATCAGCCTATTTAGACCGtttt                                                                                                                             >  2:20975/1‑250 (MQ=255)                                                                                                    aTAAAGTAACCTTGATCGAAGCCATTTACTCGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAAGCAACAATCGTATCAGAATAGATACGTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAATCGGGGAAATTCTTCTCGGCTGACTCAGTTATTTCATGTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAAGGCCCTGAATCAGCCTATTTAGACCGTTTCTTCg                                                                                                                         <  2:116506/251‑1 (MQ=25)                                                                                                                           aTTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGATATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTCTTTAGACCGTTTCTTCGCCATTTAAGTCGTTATCCCCAGt                                                                                                  >  1:161458/1‑251 (MQ=255)                                                                                                                                              gAAAAACTTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTGAAGGCGTTATCCCCAGTTTTTAGTGAGAtctct                                                                                  <  2:191503/248‑1 (MQ=255)                                                                                                                                                   aCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCTGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTg                                                                           >  2:167241/1‑250 (MQ=255)                                                                                                                                                    cGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGAc                                                                         >  1:240665/1‑251 (MQ=255)                                                                                                                                                         cAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATc                                                                    <  1:25578/251‑1 (MQ=255)                                                                                                                                                          aGCATAAATGATCCTGCGCACCAATCAACAACCGTACCAGAATAGATACTCTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATc                                                                    >  1:228394/1‑250 (MQ=255)                                                                                                                                                             aTAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCCttt                                                                >  2:129761/1‑251 (MQ=255)                                                                                                                                                             aTAAATGATCCTGAGCACCAATCAACAACAGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGCTTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCAttt                                                                >  2:134561/1‑251 (MQ=255)                                                                                                                                                               aaaTGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTGTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATTTTTTAGCCGATTTTGTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTg                                                               <  1:152185/250‑1 (MQ=255)                                                                                                                                                                   gATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAGCCCCAGTTTTTAGTGAGATCTCGCCCACTGACGTATCATTTGGTCCg                                                          <  1:134561/251‑1 (MQ=255)                                                                                                                                                                           gcACCAACCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAAc                                                  >  1:78679/1‑251 (MQ=255)                                                                                                                                                                                         ccGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGt                                    <  1:136670/251‑1 (MQ=255)                                                                                                                                                                                          cGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTg                                   >  2:41026/1‑251 (MQ=255)                                                                                                                                                                                              tCAGACTAGACACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGACATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCCGCGTGAAt                                >  2:159104/1‑250 (MQ=255)                                                                                                                                                                                               cAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATa                               >  2:14093/1‑250 (MQ=255)                                                                                                                                                                                                  aaTAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACAt                           <  1:25894/251‑1 (MQ=255)                                                                                                                                                                                                                 tAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTAt             <  2:23278/250‑1 (MQ=38)                                                                                                                                                                                                                         tgaggggatttaAGGAATGAGAGAATAAGCGGGCAAATTCTTCTCGGCTGCATGAGTCATTTAATTTCTTCATGTTTGAGCCGATCTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCAATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCCTTTGGTCCGCAAGAAACAGGTTGGCCAGCGTGAATACCCTCGCCAGTTGGTTATCGTCTTTcc    >  2:37634/10‑250 (MQ=11)                                                                                                                                                                                                                            gTTTTATTAAGGCAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGCTTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCTCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGc  >  1:92290/1‑250 (MQ=17)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                TCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGC  >  NC_000913/2101520‑2101988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.