Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,176,844	IS4 (–) +13 bp	coding (349‑361/855 nt)	gatY ←	tagatose‑1,6‑bisphosphate aldolase 2

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2176844 =	0 (0.000)	21 (0.890)	20/494	0.3	100%	coding (361/855 nt)	gatY	tagatose‑1,6‑bisphosphate aldolase 2
	?	NC_000913	= 4503515	NA (NA)					noncoding (1/1426 nt)	IS4	repeat region
*	?	NC_000913	= 2176856	0 (0.000)	26 (1.100)	24/496	0.1	100%	coding (349/855 nt)	gatY	tagatose‑1,6‑bisphosphate aldolase 2
	?	NC_000913	4502090 =	NA (NA)					noncoding (1426/1426 nt)	IS4	repeat region

ATGTTCTCCAGTCTAGAAAAATCAAGCGCCGGTGCGCTGGCATACATCCCATGAGCCGTGCCGATGGCGACCGCCAGGGAATCAATTCCGGTTGCCTCGGCAAATTCACGCGCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2176615‑2176856 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCATTCTCATCACCATTCCGCATGATTCTGAGAAACTCAGTTGATTCGGCC  >  NC_000913/4502090‑4502329                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    ATGTTCTCCAGTCTAGAAAAATCAAGCGCCGGTGCGCTGGCATACATCCCATGAGCCGTGCCGATGGCGACCGCCAGGGAATCAATTCCGGTTGCCTCGGCAAATTCACGCGCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCC                                                                                                                                                                                                                                           >  2:3630/1‑249        CCAGTCTAGAAAAATCAAGCGCCGGTGCGCTGGCATACATCCCATGAGCCGTGCCGATGGCGACCGCCAGGGAATCAATTCCGGTTGCCTCGGCAAATTCACGCGCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAG                                                                                                                                                                                                                                     <  1:206855/248‑1              TAGAAAAATCAAGCGCCGGTGCGCTGGCATACATCCCATGAGCCGTGCCGATGGCGACCGCCAGGGAATCAATTCCGGTTGCCTCGGCAAATTCACGCGCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAAC                                                                                                                                                                                                                            >  1:33572/1‑251                  AAAATCAAGCGCCGGTGCGCTGGCATACATCCCATGAGCCGTGCCGATGGCGACCGCCAGGGAATCAATTCCGGTTGCCTCGGCAAATTCACGCGCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGAC                                                                                                                                                                                                                        <  1:215345/251‑1                    AATCAAGCGCCGGTGCGCTGGCATACATCCCATGAGCCGTGCCGATGGCGACCGCCAGGGAATCAATTCCGGTTGCCTCGGCAAATTCACGCGCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTG                                                                                                                                                                                                                      <  1:198103/251‑1                    AATCAAGCGCCGGTGCGCTGGCATACATCCCATGAGCCGTGCCGATGGCGACCGCCAGGGAATCAATTCCGGTTGCCTCGGCAAATTCACGCGCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTG                                                                                                                                                                                                                      <  1:198108/251‑1                     ATCAAGCGCCGGTGCGCTGGCATACATCCCATGAGCCGTGCCGATGGCGACCGCCAGGGAATCAATTCCGGTTGCCTCGGCAAATTCACGCGCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGG                                                                                                                                                                                                                     <  1:81737/251‑1                                           aACATCCCATGAGCCGTGCCGATGGCGACCGCCAGGGAATCAATTCCGGTTGCCTCGGCAAATTCACGCGCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGAAACGGACTGGCTCTTTTTCGGGGCTGTGGGGT                                                                                                                                                                                               <  2:10644/250‑1                                                  CATGAGCCGTGCCGATGGCGACCGCCAGGGAATCAATTCCGGTTGCCTCGGCAAATTCACGCGCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCCTTGAATTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTACTTCC                                                                                                                                                                                         >  1:153808/1‑250                                                         CGTGCCGATGGCCACCGCCAGGGAATCAATACCGGTTGCCGCGGCAAATTCACGCGCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTC                                                                                                                                                                                 <  2:6368/251‑1                                                                      ACCGCCAGGGAATCAATTCCGGTTGCCTCGGCAAATTCACGCGCCTGAGCGGGGTTGGCGTACAACGCATCGGCTTCATTGACTCGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACACCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGCACTTCCAGGGCCTCTCCTTTACCACTC                                                                                                                                                                    >  2:29168/1‑251                                                                                       TCCGGTTGCCTCGGCAAATTCACGCGCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCATCTCCTTTACCACTCTCGGGAAGGCCCTTTCC                                                                                                                                                   >  1:126304/1‑251                                                                                                      AAATTCACGCGCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAAT                                                                                                                                    >  1:97639/1‑251                                                                                                                GCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACGCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTT                                                                                                                          <  1:133879/251‑1                                                                                                                          GGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCACAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACT                                                                                                                >  2:65363/1‑251                                                                                                                                     AACGCATCGGCTTCATTGGCTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCCGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTGGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGTCCTCTCCGTTACCACTCTCGGGAAGGCCCTGTCCCTTCTTGTCTGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGC                                                                                                     <  2:238814/251‑1                                                                                                                                       CGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGC                                                                                                   <  1:77675/251‑1                                                                                                                                                                      TCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGC                                                                      <  2:48239/249‑1                                                                                                                                                                      TCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAA                                                                        <  1:46398/247‑1                                                                                                                                                                      TCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAA                                                                        <  2:33572/247‑1                                                                                                                                                                                                CGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCCCCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCATTCTCATC                                          <  2:173723/251‑1                                                                                                                                                                                                CGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCATTCTCATC                                          >  1:31675/1‑251                                                                                                                                                                                                 GCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCTATACTTGCAAGATCGCGCATCAGCTCCGTTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCATTCTCATCA                                         <  2:115457/251‑1                                                                                                                                                                                                                       GATGGCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCATTCTCATCACCATTCCGCATGATTCTGAGA                    >  2:51125/1‑250                                                                                                                                                                                                                           GCAAAAATCCACCACCTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCATTCTCATCACCATTCCGCATGATTCTGAGAAACTC               >  1:144191/1‑251                                                                                                                                                                                                                                          CTCTTTGACTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCATTCTCATCACCATTCCGCATGATTCTGAGAAACTCAGTTGATTCGGCC  >  2:155800/1‑249                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    ATGTTCTCCAGTCTAGAAAAATCAAGCGCCGGTGCGCTGGCATACATCCCATGAGCCGTGCCGATGGCGACCGCCAGGGAATCAATTCCGGTTGCCTCGGCAAATTCACGCGCCTGAGCGGGGTTGGTGTACAACGCATCGGCTTCATTGACTTGCACATCATCTTCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2176615‑2176856 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCATTCTCATCACCATTCCGCATGATTCTGAGAAACTCAGTTGATTCGGCC  >  NC_000913/4502090‑4502329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.