Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,271,728	Δ1 bp	coding (122/1101 nt)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,271,728	0	G	.	100.0%	76.1 / NA	17	coding (122/1101 nt)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (9/8);  total (9/8)

TGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCTGAAATCAAACTGACTTCAAGAATAACCACTGAAAGGCTAAGAATAAGCGAACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGG‑TTTTTACCGCCTCTTGAGCATTTGACA‑TTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTT  >  NC_000913/1271484‑1271965                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                tGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCTGAAATCAAACTGACTTCAAGAATAACCACTGAAAGGCTAAGAATAAGCGAACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGCAGATTGATG‑CAATGAc                                                                                                                                                                                                                                          >  1:140938/1‑251 (MQ=255)      aGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCTGAAATCAAACTGACTTCAAGAATAACCACTGAAAGGCTAAGAATAAGCGAACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATG‑CAATGACCACTg                                                                                                                                                                                                                                     >  2:175695/1‑251 (MQ=255)                                              gagCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCTGAAATCAAACTGACTTCAAGAATAACCACTGAAAGGCTAAGAATAAGCGAACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATAACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGCTTGATG‑CAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTAcc                                                                                                                                                                                               >  1:131804/1‑249 (MQ=255)                                                                       gcggcgTCGCCGGTTGCCATTAAAGCTGAAATCAAACTGACTTCAAGAATAACCACTGAAAGGCTAAGAATAAGCGAACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATG‑CAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGaa                                                                                                                                                                    <  2:181679/251‑1 (MQ=255)                                                                         ggcgTCGCCGGTTGCCATTAAAGCTGAAATCAAACTGACTTCAAGAATAACCACTGAAAGGCTAAGAATAAGCGAACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATG‑CAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGt                                                                                                                                                                  >  2:5262/1‑251 (MQ=255)                                                                         ggcgTCGCCGGTTGCCATTAAAGCTGAAATCAAACTGACTTCAAGAATAACCACTGAAAGGCTAAGAATAAGCGAACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATG‑CAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGt                                                                                                                                                                  <  1:62872/251‑1 (MQ=255)                                                                         ggcgTCGCCGGTTGCCATTAAAGCTGAAATCAAACTGACTTCAAGAATAACCACTGAAAGGCTAAGAATAAGCGAACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATG‑CAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGt                                                                                                                                                                  >  2:126081/1‑251 (MQ=255)                                                                                           tAAAGCTGAAATCAAACTGACTTCAAGAATAAACACTGACCAGCTAAGAATAAGCGAACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATG‑CAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGACAATAAGCGAAGTCTCCTGGTGGCggtgctt                                                                                                                                                >  2:20242/1‑246 (MQ=255)                                                                                                        gaaCTGACTACCAGACTAACCACTGGAAGGCTAAGTATATGCGAACCGTAAGGTTCTCCAAGGAGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGACGCTCCTTAAAATACCAATAAGCGCAAGCAGATTGATG‑AAATGACCACTGGTCGTGTCTGGCTGCTTCCCCACAGGAACAGCCCTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCggagggtgttgttcctcttg                                                                                                                                   >  2:219252/2‑233 (MQ=255)                                                                                                         aaCTTACTTCAAGAATAACCACGGAAAGGCTAAGAATAAGGGAACGGTAAGGTTGTCCCAGGCGAGGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTGCTTAAAAGACCAATAAGCGCAAGAAGATTGATG‑CAATGACCACTGGTAGTGTCGGGCTTCTTCCCCACAGGAACAGCACGCCCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGGGGCGGG‑TTTTTACCGCCTGTTGAg                                                                                                                                 <  2:229413/251‑1 (MQ=255)                                                                                                           cTGACTTCAAGAATAACCACTGAAAGGCTAAGAATAAGCGAACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATG‑CAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGG‑tgttttctgcaccttttgt                                                                                                                                >  2:67364/1‑236 (MQ=255)                                                                                                                  cAAGAATAACCACTGAAAGGCTAAGAATAAGCGAACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATG‑CAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGG‑TTTTTACCGCCTCTTGAGCATTTGAc                                                                                                                         <  1:234356/250‑1 (MQ=255)                                                                                                                                  aaGGCTAAGAATAAGCGAACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATG‑CAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAATTCTCCTTGTGGCGGG‑TTTTTACCGCCTCTTGAGCATTTGACA‑TTATGGTTATCCCttt                                                                                                       <  1:157167/251‑1 (MQ=255)                                                                                                                                                     ccGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACAAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATG‑CAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGG‑TTTTTACCGCCTCTTGAGCATTTGACA‑TTATGGTGATCCCTTTGCAGATGAATTTATCGaaa                                                                                    <  1:191148/251‑1 (MQ=255)                                                                                                                                                           ggTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATG‑CAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGG‑TTTTTACCGCCTCTTGAGCATTTGACA‑TTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTaa                                                                              <  1:5262/251‑1 (MQ=255)                                                                                                                                                                                                          aaaGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATG‑CAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGG‑TTTTTACCGCCTCTTGAGCATTTGACA‑TTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATAGCGGTAACa                                <  1:175695/250‑1 (MQ=255)                                                                                                                                                                                                                                       cAAGAAGATTGATG‑CAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTAC‑GCCCCTTTAGCCTTTTTCAATTTGGGTTTTCCCTT‑GCCGAAGAATTTTTCGAAAATGTAAAAAAAAAGGAGGAAAAAAAAAAAAAAATGGTTGGGTATTGGTAACATTTTTCGAAATTTTATCCCCCTGTCGAttt  >  1:79187/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                TGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCGGTTGCCATTAAAGCTGAAATCAAACTGACTTCAAGAATAACCACTGAAAGGCTAAGAATAAGCGAACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGG‑TTTTTACCGCCTCTTGAGCATTTGACA‑TTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTT  >  NC_000913/1271484‑1271965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑