Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,025,601	(T)5→4	coding (1251/1401 nt)	xanQ →	xanthine:H(+) symporter XanQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,025,601	0	T	.	100.0%	104.8 / NA	23	coding (1251/1401 nt)	xanQ	xanthine:H(+) symporter XanQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (9/14);  total (9/14)

CGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACA‑CGTTAAAAGCGGTACGAGG‑CAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAG  >  NC_000913/3025367‑3025844                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      cGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCGTCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCctc                                                                                                                                                                                                                                      <  1:34569/251‑1 (MQ=255)  gggCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGCATGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACCTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCctct                                                                                                                                                                                                                                     >  1:230322/1‑251 (MQ=255)     cGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTAtt                                                                                                                                                                                                                                  <  1:6709/251‑1 (MQ=255)       aaCCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTAttt                                                                                                                                                                                                                                 >  1:22091/1‑250 (MQ=255)             cgcgGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGTATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATGGTCGCCACTTCTTTAGGTTTAGGGCGTGGCGTCTCCTACGATCCCTAAATGTT‑AAAATATTGCCAGCCCCTATTTATGTAtt                                                                                                                                                                                                                          <  2:12775/251‑1 (MQ=255)              gcgGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTa                                                                                                                                                                                                                         <  1:219295/251‑1 (MQ=255)                         ggTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAAccc                                                                                                                                                                                                              >  2:25382/1‑251 (MQ=255)                            tATCCTCGGCTTATTTCCGATGATTGGCGGCTTATTCACGACCATTCCCTAGGCAGTTCTGGGAGGCGCAATGACGTTGATGATTTCCATGATTGCCATCGCAGGGATTCGCATCATCCTCCCCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCACGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTCTTAGATGACAACCCTAt                                                                                                                                                                                                           >  1:216222/1‑251 (MQ=255)                                          gtCCGATGATTGGCTGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCTCCACTGCTTTAGGTTTAGTGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCggg                                                                                                                                                                                              <  2:88220/249‑1 (MQ=255)                                                        ggcTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTaaatttgcttttt                                                                                                                                                                               >  1:200794/1‑240 (MQ=255)                                                                         tCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTAtcctcc                                                                                                                                                               <  2:196875/250‑1 (MQ=255)                                                                                               gcAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAAGATTATCCTCCCTGGTGGCTACCGACAGGAAAAc                                                                                                                                        <  2:200794/251‑1 (MQ=255)                                                                                                cAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTTATTGCGATTTTTTTAAATATTATCCTCCCTGGTGGCTACCGACAGGtaaa                                                                                                                                         >  2:108018/1‑249 (MQ=255)                                                                                                    gACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTc                                                                                                                                    <  1:42115/250‑1 (MQ=255)                                                                                                          gATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAAAACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTTTTTAATATTATTCTCCCTGGGGGCTACCGACAGGAAAAAGTTCTGCCttg                                                                                                                             >  2:237340/1‑249 (MQ=255)                                                                                                                      gATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGc                                                                                                                 <  1:25382/251‑1 (MQ=255)                                                                                                                                         cGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGTCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACa                                                                                               <  2:220514/250‑1 (MQ=255)                                                                                                                                                    caCCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTc                                                                                    <  2:59693/250‑1 (MQ=255)                                                                                                                                                              ttAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTc                                                                           >  1:318/1‑249 (MQ=255)                                                                                                                                                               tAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAg                                                                         <  1:59590/250‑1 (MQ=255)                                                                                                                                                                    cgcCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAac                                                                   <  1:19207/251‑1 (MQ=255)                                                                                                                                                                        gTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCTTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAGCAGTAAAGGAGTCAATGATGTCATGAGAacaca‑c                                                              <  2:75221/251‑1 (MQ=255)                                                                                                                                                                                     tGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTTAATGCGATTTTATTTAATATTATCCTCCCTTGTGGCTACCCACAGGAAAAAGGTTTTCCTGGTATTACCTCAGCGGGAGAGATGGATTAACAGTAAAAGAGGCAATGATGTCAGGAGAacaacagtttaaaacggg                                                   >  2:54781/1‑235 (MQ=255)                                                                                                                                                                                                                                    gaaattttAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGGTAAATGCGATTTTTTTAAATATTATCCTTCCTGGTGGCTACCGACAGGAAAAAGTTCTGCCTGGGATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACA‑CGGTAAAAGCGGTGCGAGG‑GAGTTTTTTTTATGTAACCCCTACGAACGATAAccccga    >  2:130464/5‑246 (MQ=255)                                                                                                                                                                                                                                       attttAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTTTGCTGGCGGGGTAACTTCGGTTTTTTTAAATAATTTCCTCCCCGGTGGGTTCCGACCGGGAAAAGTTTTGCCTGGTCTTTCCTCCGCGGAAGAGATGGATTAACAGTGAATGAGTCAAATATGTCAGGCGAA‑ACAACGTTTAAAACGGGACGAGGGCGGTTTT‑TTGATGTTACCCCTACCATAGATAACCCgggag  >  2:58404/2‑245 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      CGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACA‑CGTTAAAAGCGGTACGAGG‑CAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAG  >  NC_000913/3025367‑3025844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑