Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,448	C→T	intergenic (‑575/+834)	gatD ← / ← gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,448	0	C	T	100.0%	198.8 / NA	55	intergenic (‑575/+834)	gatD/gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/28);  total (27/28)

CCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATA  >  NC_000913/2173199‑2173691                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    ccGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGc                                                                                                                                                                                                                                                  >  2:173472/1‑251 (MQ=255) ccGAGATTAAGGTGCGGAACAGATTTCAACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATTGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGAAACGCCGGATCAAGGCCAATCAGGAACTCCTGAACGCCGAACTTCGCCTGTAAAGGACTACGAGCTTGc                                                                                                                                                                                                                                                  >  2:173454/1‑251 (MQ=255)   gagaTTAAGGTGCGGAACAGATTTCCCCGAGGCAGGGCGACCGCCATCGCCACAAAGAAGCCGAGGGTGGCAAGATCGCCAACCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGT‑TGAGGGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAgcttgctt                                                                                                                                                                                                                                                <  2:24883/251‑1 (MQ=255)    agaTTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCTTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTa                                                                                                                                                                                                                                               <  2:167765/251‑1 (MQ=255)    agaTTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTa                                                                                                                                                                                                                                               <  1:28098/251‑1 (MQ=255)                      gATTTCCACGATGCACGGCGACCGCCATCGCCACGCAGAAGCCGATGGTGGCAAGATCGCCAACCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GTCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAAcc                                                                                                                                                                                                                              >  1:331720/1‑250 (MQ=255)                            cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCatg                                                                                                                                                                                                                       >  2:556928/1‑251 (MQ=255)                            cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGAGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCatg                                                                                                                                                                                                                       >  1:457987/1‑251 (MQ=255)                                   cACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCTAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGtt                                                                                                                                                                                                                <  1:60532/251‑1 (MQ=255)                                    cgggcacccgccaccgccaCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACAAAAAAAGAAATAAAAATGG‑TGAGTGGGAAAAAAACCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGttt                                                                                                                                                                                                               <  2:578823/244‑1 (MQ=255)                                      ggCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTACACCATCCATGATGGGtttc                                                                                                                                                                                                              >  2:253320/1‑249 (MQ=255)                                           cccccaccGCCACAAAGAAGCCGATGGTGGAAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATAAAAAGGG‑TGAGTGGGAAAAAAATCAGGCTTG‑CCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAcc                                                                                                                                                                                                        <  2:450998/244‑1 (MQ=255)                                                        gaagaaGCCGATGGTGGCAAGATCGCCAAACGGCCGCACCTGATTACCCGGCACACAAACAGCAATTACAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTCAACGACTCCGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagcc                                                                                                                                                                                           >  1:270868/1‑250 (MQ=255)                                                                         cAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGtt                                                                                                                                                                          >  2:157069/1‑251 (MQ=255)                                                                         cAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGtt                                                                                                                                                                          >  2:447368/1‑251 (MQ=255)                                                                               cGCCAAAGGGCAGCACCTGATTACCCGCCACACAAACAGCAATAAAATTGT‑TGAGTGGGATAAAAACCAGGCTTG‑CCGTTACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATTATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTGTTTAcc                                                                                                                                                                    <  1:190915/251‑1 (MQ=255)                                                                                    aaCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                                                                                                 >  1:553579/1‑249 (MQ=255)                                                                                              cctgattcccgggcaacaaaaCAGCAATAAAAAGGG‑GGAGTGGAAAAAAAAACAGGCTTG‑CCGAT‑CC‑CCCCGCCGTATGTCCCAGCAGAAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATaca                                                                                                                                                     <  2:123884/233‑1 (MQ=255)                                                                                               cTGATTACCCGGCACACAAACAGCAATTAACATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATacac                                                                                                                                                    >  2:239587/1‑251 (MQ=255)                                                                                                   ttccccGGCACAAAAACAGCAAT‑AAAATGGGTGGGTGGAAAAAAAATC‑GGCTGGGCCGATCCCACC‑‑GCCGTATTGCCCAGCAGCAACCCCGGAGCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCGCTGCCGCTGTTTTTGCCGCCAGCTGCAATACACCtt                                                                                                                                                 <  2:547968/247‑1 (MQ=255)                                                                                                         ggCAAACAAACAGCAATAAAAAGGG‑GGAGGGGGAAAAAAACCAGCCTTG‑CCAATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATc                                                                                                                                          <  2:3220/251‑1 (MQ=255)                                                                                                           cacacaAACAGCAATTAAAATGG‑TGAGTGGGATTAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGt                                                                                                                                        >  1:151803/1‑251 (MQ=255)                                                                                                             cacaGACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTa                                                                                                                                       >  2:35479/1‑250 (MQ=255)                                                                                                             cacaAACAGCAATTAAAATGC‑TGAGTGGGAAAAAAATCAGGCTTG‑CCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTaa                                                                                                                                      <  1:277085/251‑1 (MQ=255)                                                                                                                 aaaaGCAATTAAAATGG‑GGAGTGTGATAAAAATCAGGTTTA‑CCGAAACCACC‑‑GCCGTAGGTCCCAGCAGAAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAGCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCTCCAGCTGCAATACACCTTTGACATCGTAACCcg                                                                                                                                  <  1:40637/248‑1 (MQ=255)                                                                                                                   cAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCcgcg                                                                                                                                >  1:187094/1‑251 (MQ=255)                                                                                                                        ataaaaaGGG‑TGAGGGGAATAAAAATCGGGCTGG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGAt                                                                                                                           <  1:180084/248‑1 (MQ=255)                                                                                                                                            tAAAAATCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGACAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCCATACACCTTTGACATCGTAACCCGCGAGGATGCCTATAATCAGCCCCATc                                                                                                        >  1:427235/1‑251 (MQ=255)                                                                                                                                              aaaaacagggctG‑CCGGTACC‑CCCCGCCGTATGTCCCAGCAGCAGCGCCGGATCAAGGCCAAGCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATca                                                                                                       <  2:528120/240‑1 (MQ=255)                                                                                                                                                  tCAGGCTTG‑CCGATACCACC‑‑GCCGTATGTTACAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTGACCGCCGACCTTCGCCTGTCAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACaaa                                                                                                   >  2:542234/1‑250 (MQ=255)                                                                                                                                                   cAGGCTTG‑CCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAc                                                                                                 >  1:204550/1‑251 (MQ=255)                                                                                                                                                     ccaggctggcagacacccccGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACaaaa                                                                                                  <  2:395316/235‑1 (MQ=255)                                                                                                                                                         tG‑CCGATACCATC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATAAGGAACTCCTGACCGACGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGACAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCACATCACAAAACCCaagg                                                                                           >  2:488244/1‑248 (MQ=255)                                                                                                                                                            ccGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGAGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTg                                                                                         <  1:370261/251‑1 (MQ=255)                                                                                                                                                                gatacccccGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGAc                                                                                       >  1:383854/6‑251 (MQ=255)                                                                                                                                                                    ccaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCTCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGc                                                                                   <  1:570474/248‑1 (MQ=255)                                                                                                                                                                      cccGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTc                                                                                 <  1:461096/251‑1 (MQ=255)                                                                                                                                                                         gccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGcc                                                                              >  1:122750/1‑251 (MQ=255)                                                                                                                                                                         gccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGcc                                                                              >  1:118263/1‑251 (MQ=255)                                                                                                                                                                                 ccccAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGAc                                                                      <  2:58749/250‑1 (MQ=255)                                                                                                                                                                                  cccAGCAGCAACGCCGTATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTGTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGAcc                                                                     <  2:108236/251‑1 (MQ=255)                                                                                                                                                                                       cagcaACGCCGGATCAAGGCCACTCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGCCCAAAtt                                                                >  2:562411/1‑251 (MQ=255)                                                                                                                                                                                         gcaACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTg                                                              <  1:462033/251‑1 (MQ=255)                                                                                                                                                                                           aaCGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGCCCAAATTTgcg                                                            >  2:222195/1‑251 (MQ=255)                                                                                                                                                                                              gCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGCCAGGCTCGCCAAATGGACCAAATTTGCGCTg                                                         >  1:195146/1‑251 (MQ=255)                                                                                                                                                                                               ccGGATCAAGGCCAATCAGGAACTCCGGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGa                                                        <  2:69398/251‑1 (MQ=255)                                                                                                                                                                                                   aTCAAGGCCAATCAGGACCTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCTTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAatat                                                    <  1:446256/251‑1 (MQ=255)                                                                                                                                                                                                   aTCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAatat                                                    <  1:31455/251‑1 (MQ=255)                                                                                                                                                                                                     cAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATAtc                                                   <  1:447368/250‑1 (MQ=255)                                                                                                                                                                                                      aaGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATAtcgt                                                 <  1:409032/251‑1 (MQ=255)                                                                                                                                                                                                                  gaactaatGACCCCCGAACTTCGCCTGTAAACGCCTTCGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGATTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCACTACACCTTTGCCATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAAAGGACCAAATTTGCGCTGAATATCGTCGGCGCTaactt                                     >  2:558045/8‑248 (MQ=255)                                                                                                                                                                                                                              gccgAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTa                          >  2:64129/1‑250 (MQ=255)                                                                                                                                                                                                                                     ttCGCCTGTAAACGACTACGTGCTTGCTTAGCGATGGGCGTTAAACCATCCACGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACACCGTAACCCGCGAGGAAGCCGATAATCAGCCACATCACAAAACACACGGTGACAGGCTCGCCAAATGGACCAAATTTCCGCTGAATATCGTCGGCGCTACATTTACTTCGGTTAAAGCCTgg                  >  2:540863/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cGACTACGAGCTTGCTTAGCGATGGTCGTTAAACCCTCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACTCCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATATAATTCGGTTAGCTCCTGGGATTTTCTCgat      <  2:582109/251‑1 (MQ=255)                                                                                                                                                                                                                                                      tggAGCTTGCTTGGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATa  <  2:485686/248‑1 (MQ=255)                                                                                                                                                                                                                                                      aCGAGCTTGCTTAGCGATGGGCGTTAAACCTTCCATGAGGGGTTTAATCACCCGTGGCATTAGCAGCGGCACTGCCGCCGTTTTTACCGCCAGCTGCAATACGCCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAGCCCACGGTGACAGGCTCGACAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAGGTCGGTTAACGCCTGGGATTTTCTCGATGATa  <  1:164688/250‑1 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    CCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTG‑CCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATA  >  NC_000913/2173199‑2173691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑