Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,052,885	G→A	intergenic (+871/‑758)	yeeJ → / → shiA	inverse autotransporter adhesin/shikimate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,052,885	0	G	A	100.0%	140.6 / NA	39	intergenic (+871/‑758)	yeeJ/shiA	inverse autotransporter adhesin/shikimate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/21);  total (18/21)

TAGATTTTCTGCAATCGATAATGAAAATGTACGTTCCTGCGAGCTACCTTCTAAATTGAACGCAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGACTAATGTTAATTTAGTCTGTTCAAGTTTAAGCGCATTAAAGACTTCTAA  >  NC_000913/2052639‑2053127                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   tAGATTTTCTGCAATCGATAATGAAAATGTACGTTCCTGCGAGCTACCTTCTAAATTGAACGCAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGc                                                                                                                                                                                                                                                >  2:479895/1‑251 (MQ=255)  aGATTTTCTGCAATCGATAATGAAAATGTACGTTCCTGCGAGCTACCTTCTAAATTGAACGCAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGc                                                                                                                                                                                                                                                <  1:309140/250‑1 (MQ=255)           gCAATCGATAATGAAAATGTACGTTCCTGCGAGCTACCTTCTAAATTGAACGCAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCAt                                                                                                                                                                                                                                         <  1:452940/248‑1 (MQ=255)                 gATAATGAAAATGTACGTTCCTGCGAGCTACCTTCTAAATTGAACGCAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATAcc                                                                                                                                                                                                                                >  2:557337/1‑251 (MQ=255)                                 gTTCCTGCGAGCTACCTTCTAAATTGAACGCAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTc                                                                                                                                                                                                                >  2:112438/1‑251 (MQ=255)                                    ccTGCGAGCTACATTCTAAATTGAACGCAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGGGGCAATTCATATATTGGCCGGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGtt                                                                                                                                                                                                             <  2:162011/251‑1 (MQ=255)                                               ccTTCTAAATTGAACGCAATGTAAGATCCTAACGATCTCATTTCCTCGCGCACCTCATCGAGTCCATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCaa                                                                                                                                                                                                  <  2:460846/251‑1 (MQ=255)                                                     aaaTTGAACGCAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAactgact                                                                                                                                                                                            >  1:399005/1‑251 (MQ=255)                                                      aaTTGAACGCAATGTAAGATCCGAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATAGATTGGCCTGACGGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGTACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTAGTCCAACATCCTGAAAATTCGTTTTTGCTTTCAactgactg                                                                                                                                                                                           <  1:351728/251‑1 (MQ=255)                                                       aTTGAACGCAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAActgactga                                                                                                                                                                                          <  2:399005/251‑1 (MQ=255)                                                               cAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTAACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCCCGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCACCTGACTGATAAATAAc                                                                                                                                                                                  >  1:79404/1‑251 (MQ=255)                                                                      aGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGttt                                                                                                                                                                           <  1:103653/251‑1 (MQ=255)                                                                        aTCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCCCTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTc                                                                                                                                                                         >  1:61677/1‑251 (MQ=255)                                                                                   cGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGACAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCttttt                                                                                                                                                              >  1:23320/1‑251 (MQ=255)                                                                                   cGCATTACCCCGCTCACCTCATAGATTACAACCTCTCTTAGCGGCAATTCATATATTGGCCTGACTGTTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCttttt                                                                                                                                                              <  2:481245/251‑1 (MQ=255)                                                                                    gCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCttttt                                                                                                                                                              >  2:511353/1‑250 (MQ=255)                                                                                               cgcACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTc                                                                                                                                                  <  2:241936/251‑1 (MQ=255)                                                                                                               cATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGc                                                                                                                                  <  2:203522/251‑1 (MQ=255)                                                                                                                    ccccTTAGTGGCAATTCATATTTTGCCCTGCCTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGTAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATTCCAACAACCCGAAAATGCGTTTTTGCTTTCAACTGACTGATAAAGAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCTGGGTAcc                                                                                                                             <  2:47537/248‑1 (MQ=255)                                                                                                                               cAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCtgtg                                                                                                                  >  1:435239/1‑251 (MQ=255)                                                                                                                                 aatCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTAGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGtt                                                                                                                >  1:26146/3‑251 (MQ=255)                                                                                                                                     atatatTGGCCTCACGGCTGTAAAACCCGCCTCACGCATCATAAATTCCCATGTCATATGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTAttt                                                                                                            <  1:133920/251‑3 (MQ=255)                                                                                                                                                     gctgGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCGCAATGCAAAGATGCGGCGTACCGTATTGCTGTGTTATGGTCTTTGCTAAATCtt                                                                                             <  2:250332/250‑1 (MQ=255)                                                                                                                                                        ggAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTAt                                                                                         <  2:560325/251‑1 (MQ=255)                                                                                                                                                        ggAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCGGTGTTATGTTTTTTGCTAAATCtttt                                                                                           <  1:133906/249‑1 (MQ=255)                                                                                                                                                              cccGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATCCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCttt                                                                                   >  2:400448/1‑251 (MQ=255)                                                                                                                                                                cGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCCACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCtttt                                                                                  >  1:27017/1‑250 (MQ=255)                                                                                                                                                                 gCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTa                                                                                 >  2:438275/1‑250 (MQ=255)                                                                                                                                                                             atAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTGCTTTTAATGTTGCGTGATc                                                                    <  1:130214/251‑1 (MQ=255)                                                                                                                                                                               aaaTGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCt                                                                  >  2:113811/1‑251 (MQ=255)                                                                                                                                                                                     ccATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCAt                                                              >  2:304245/1‑249 (MQ=255)                                                                                                                                                                                               ggTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGAc                                                  >  1:276710/1‑251 (MQ=255)                                                                                                                                                                                                     ggAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGACTAATGt                                            <  1:77664/251‑1 (MQ=255)                                                                                                                                                                                                                         cATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGACTAATGTTAATTTAGTCTGTTCAAGtt                        <  2:304536/251‑1 (MQ=255)                                                                                                                                                                                                                         cATACTGGACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGACTAATGTTAATTTAGTCTGTTCAAGtt                        >  1:36155/1‑251 (MQ=255)                                                                                                                                                                                                                               ggACGCGTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGACTAATGTTAATTTATTCTGTTCAAGTTTAAgc                   <  1:479895/250‑1 (MQ=255)                                                                                                                                                                                                                                    cgTTCTTGCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGACTAATGTTAATTTAGTCTGTTCAAGTTTAAGCTCATTa             <  1:418748/251‑1 (MQ=255)                                                                                                                                                                                                                                           gCACAAAGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGACTAATGTTAATTTAGTCTGTTCAAGTTTAAGCGCATGAAAGACtt      <  1:112438/251‑1 (MQ=255)                                                                                                                                                                                                                                                aaGGGGAAAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGACTAATGTTAATTTAGTCTGTTCAAGTTTAAGCGCATTAAAGACTTCTaa  >  1:407136/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   TAGATTTTCTGCAATCGATAATGAAAATGTACGTTCCTGCGAGCTACCTTCTAAATTGAACGCAATGTAAGATCCTAACGATCGCATTTCCTCGCGCACCTCATCGAGTACATCCTCACTTAGTGGCAATTCATATATTGGCCTGACTGCTGGAAAACCCGCCTCACGCATCATAAATGCCCATGTCATAGGTACGGGAGCCCGGAGTTTCTGATCCATACTGGACGCGTTCTTGCACAAAGGGGAGAAGCAATTCATGGTTATACCAACAACCTGAAAATTCGTTTTTGCTTTCAACTGACTGATAAATAACATCGTTTTCAGGTTCTTTTTACGCATCCCCTCAATGCAAAGATCCGGCGTACCGTATTGCTGTGTTATGTTCTTTGCTAAATCTTTTATTTCTTTTAATGTTGCGTGATCCTGCATAGTCATTGTGACTAATGTTAATTTAGTCTGTTCAAGTTTAAGCGCATTAAAGACTTCTAA  >  NC_000913/2052639‑2053127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑