Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,849,629	T→C	L265L (TTA→CTA)	sppA →	protease IV, a signal peptide peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,849,629	0	T	C	100.0%	230.9 / NA	61	L265L (TTA→CTA)	sppA	protease IV, a signal peptide peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/31);  total (30/31)

ACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTTTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAAT  >  NC_000913/1849390‑1849874                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      ccGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGCTACGTATAAATCTGCCGTTGAACCTTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCTCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATGCTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCACGGGTTGCTTGATGGTCTAACCAAAAcc                                                                                                                                                                                                                                            <  2:347375/250‑1 (MQ=255)        gTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGCTGCTTGAGGGTCTAACCAAAACCGGTGGCg                                                                                                                                                                                                                                     >  2:437291/1‑251 (MQ=255)             acAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTTCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATAcc                                                                                                                                                                                                                                <  2:537212/251‑1 (MQ=255)              cAAATCGGTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTAGTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATAcct                                                                                                                                                                                                                               <  2:427075/251‑2 (MQ=255)                   cGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGaa                                                                                                                                                                                                                           <  1:432580/250‑1 (MQ=255)                       gCTGGATAAGCTGAAAGTTGCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATg                                                                                                                                                                                                                      <  1:171869/251‑1 (MQ=255)                              aaGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTgg                                                                                                                                                                                                                >  1:107723/1‑250 (MQ=255)                               aGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGaa                                                                                                                                                                                                              <  2:112343/251‑1 (MQ=255)                                gCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGaaa                                                                                                                                                                                                             <  2:255138/251‑1 (MQ=255)                                  tGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAAc                                                                                                                                                                                                           >  2:53848/1‑251 (MQ=255)                                          tCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGt                                                                                                                                                                                                   >  1:473327/1‑251 (MQ=255)                                           ccACCCATGTGGTCCGCGTGGGTACGTATAAATCTGCCGGTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGTTGAGCTGTGGCAAAACTATCTGAATACTGTTTCCGCTAACCTGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGTGTGGCTTGAGGGTCGAGCTAAAACCGGGGGCAATGCCGCGAAATATGCACtatagaacaatctggt                                                                                                                                                                                                   <  2:85752/250‑16 (MQ=255)                                            cACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCg                                                                                                                                                                                                 >  2:181847/1‑251 (MQ=255)                                             aCCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCg                                                                                                                                                                                                 >  2:219528/1‑250 (MQ=255)                                                        ccGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCTCTGGCAt                                                                                                                                                                                     >  2:456453/1‑251 (MQ=255)                                                           cgTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAAACGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAAAATGCACTTGAACACAAGCTGGTCGATGCACTGGCATCg                                                                                                                                                                                   >  2:142824/1‑250 (MQ=255)                                                            gTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCg                                                                                                                                                                                   >  2:416346/1‑249 (MQ=255)                                                            gTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAg                                                                                                                                                                                 <  1:450339/251‑1 (MQ=255)                                                              gggTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTg                                                                                                                                                                               <  1:189820/251‑1 (MQ=255)                                                                gTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCg                                                                                                                                                                             <  2:66898/251‑1 (MQ=255)                                                                               ccGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGc                                                                                                                                                               >  1:528668/1‑250 (MQ=255)                                                                                   tGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAATCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTg                                                                                                                                                           <  1:194813/250‑1 (MQ=255)                                                                                   tGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTg                                                                                                                                                           <  1:471381/250‑1 (MQ=255)                                                                                    gAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTTGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGATTGCGGAAATCGAAAAAGCACTGAc                                                                                                                                                         <  1:115820/251‑1 (MQ=255)                                                                                          tttATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCaa                                                                                                                                                      >  1:251880/1‑248 (MQ=255)                                                                                            tATTCGTTATGATAAATCACCGGAAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCACAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCCCTGACCAAAGAAt                                                                                                                                                 >  2:519624/1‑251 (MQ=255)                                                                                            tATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGaa                                                                                                                                                  <  1:428908/250‑1 (MQ=255)                                                                                                       atatGTCACCGGCAGCCCGCGAAGCTGACAGCCTCTGGCTTGGTGCGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCTTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAACACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCACAGAATTAGGCTGGAGt                                                                                                                                      >  1:524933/1‑251 (MQ=255)                                                                                                          tGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGTATACTGTTGCCGCTAACCTGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCTATACCGCGAAATATGCACTGGAAAAAAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGCCTGGAGTaa                                                                                                                                    <  2:385477/250‑1 (MQ=255)                                                                                                           gTCACCGGCACCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCCAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCCGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAACCCGGCGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGTCATCGTGTGCGGAAATCGAAAAAGCACTGCCCCCAGAATTCGGCTGGAGTa                                                                                                                                     >  1:525524/1‑248 (MQ=255)                                                                                                                 ggCAGCCCGCGCAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGAt                                                                                                                             >  1:140427/1‑250 (MQ=255)                                                                                                                 ggCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATa                                                                                                                            <  2:140146/251‑1 (MQ=255)                                                                                                                            aaGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATcgc                                                                                                                 >  2:475793/1‑251 (MQ=255)                                                                                                                                      ccGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGGAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGtt                                                                                                       >  2:169232/1‑251 (MQ=255)                                                                                                                                      ccGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGtt                                                                                                       <  1:464571/251‑1 (MQ=255)                                                                                                                                             aTTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTattacg                                                                                                 >  1:160041/1‑250 (MQ=255)                                                                                                                                                   gAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGc                                                                                          <  2:258084/251‑1 (MQ=255)                                                                                                                                                     gCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCAt                                                                                        <  1:516696/251‑1 (MQ=255)                                                                                                                                                        gtgGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTg                                                                                      <  2:425464/250‑1 (MQ=255)                                                                                                                                                          ggCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGaa                                                                                    <  2:322036/250‑1 (MQ=255)                                                                                                                                                                   aTCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGc                                                                           >  2:313853/1‑250 (MQ=255)                                                                                                                                                                          tACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCCGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCg                                                                   >  1:162960/1‑251 (MQ=255)                                                                                                                                                                            cTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGt                                                                 >  1:30826/1‑251 (MQ=255)                                                                                                                                                                               ttGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGAc                                                              >  2:581551/1‑251 (MQ=255)                                                                                                                                                                                 gCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGTTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGaaa                                                             <  2:426141/250‑3 (MQ=255)                                                                                                                                                                                    gCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCAt                                                         >  1:428811/1‑251 (MQ=255)                                                                                                                                                                                        aCCGGCAGCTCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCggg                                                     >  1:409530/1‑250 (MQ=255)                                                                                                                                                                                        aCCGGAAGATCCTGGCTGAGCAGGTATTCCCACCCGCGCCAGGGTTGCTTGAGGGCCTAACCAAAACCCGTGGCGCCACCGCGCAATATGCACTGGAAACCACGCTGGTCGATGCACTGGCATCGAGCGCGGAAATCGAAAAAGCACTGACCAAAGACTTCCGCTGGAGTAAGACTGATAAAAATGATCGCGCCATCCGTTATTACGATTACGAATTGAAAACGCCGGCAGCTAACGGTGCCAGCCTCggg                                                     >  2:572809/1‑250 (MQ=255)                                                                                                                                                                                          cGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTACGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGAAGATACCGGTGACAGCATCGgtgt                                                   >  1:228750/1‑251 (MQ=255)                                                                                                                                                                                               gATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCt                                              >  1:450515/1‑251 (MQ=255)                                                                                                                                                                                                 tCCCTGCTGAGCAGGAATTCCCTGGCGAGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCttt                                            >  2:243533/1‑251 (MQ=255)                                                                                                                                                                                                         gAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAAt                                      <  1:53848/249‑1 (MQ=255)                                                                                                                                                                                                         gAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATg                                     <  1:475793/250‑1 (MQ=255)                                                                                                                                                                                                            cAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGgcgc                                 <  1:492798/251‑1 (MQ=255)                                                                                                                                                                                                                 aTTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAAtt                             <  1:364454/250‑1 (MQ=255)                                                                                                                                                                                                                    cccTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTatgg                         <  1:473975/251‑1 (MQ=255)                                                                                                                                                                                                                             gcAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAgg                <  2:91993/251‑1 (MQ=255)                                                                                                                                                                                                                             gcAAGGGTTGCTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAgg                <  1:416346/251‑1 (MQ=255)                                                                                                                                                                                                                               aaGGGTTGCTTGAGGTTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGa               >  2:104931/1‑250 (MQ=255)                                                                                                                                                                                                                                       cTTGAGGGTCTAACCAAAACCGGTGGCGATACCGCGCACTATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAggg      >  2:52616/1‑251 (MQ=255)                                                                                                                                                                                                                                           aGGGTCTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAAt  <  2:459376/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      ACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTTTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAAT  >  NC_000913/1849390‑1849874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑