Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,767,014	C→T	G558S (GGT→AGT)	ydiJ ←	putative FAD‑linked oxidoreductase YdiJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,767,014	0	C	T	100.0%	223.1 / NA	63	G558S (GGT→AGT)	ydiJ	putative FAD‑linked oxidoreductase YdiJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/29);  total (34/29)

CGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACCGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAA  >  NC_000913/1766768‑1767258                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     cGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTgcgc                                                                                                                                                                                                                                                  <  1:547005/251‑1 (MQ=255)     ggATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTc                                                                                                                                                                                                                                               >  2:33977/1‑250 (MQ=255)               aGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGCATGTACTGCGCTTCACCGCGTCCAc                                                                                                                                                                                                                                    >  1:305778/1‑251 (MQ=255)               aGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCAc                                                                                                                                                                                                                                    >  2:518169/1‑251 (MQ=255)                   cTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTtc                                                                                                                                                                                                                                >  1:434626/1‑251 (MQ=255)                       ttCCAGTTTGAGTGGATCAACGCCGAGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTtcatc                                                                                                                                                                                                                             >  2:545819/1‑250 (MQ=255)                        tCCATTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCCTTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTtcatca                                                                                                                                                                                                                            <  2:425899/250‑1 (MQ=255)                        tCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTcatcat                                                                                                                                                                                                                           >  2:380292/1‑251 (MQ=255)                         ccAGTTTGAGTGGATCACCGCCGCGATCCGACAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTcatcat                                                                                                                                                                                                                           <  2:444604/250‑1 (MQ=255)                            gTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGgc                                                                                                                                                                                                                       >  1:301780/1‑251 (MQ=255)                                    ggATCCACGGCGCGATCCGCCAACCAACGCAGACATTCAAGCACCAGCGTTGCGCGCCCTTTCGGTGCATGCATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGAACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCg                                                                                                                                                                                                                 >  2:329622/1‑249 (MQ=255)                                           cgccgcGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGCCTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCAGATCGAATGCACTGCGCTTCACCGCGTCCACTTTCCTCATCGGCGCATCgtgcacttct                                                                                                                                                                                                        >  2:516199/1‑242 (MQ=255)                                            gccgcGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGTCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCTCCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTg                                                                                                                                                                                                       <  1:61459/251‑1 (MQ=255)                                            gccgcGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTg                                                                                                                                                                                                       >  1:331562/1‑251 (MQ=255)                                                  aTCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGc                                                                                                                                                                                                 >  1:29329/1‑251 (MQ=255)                                                      gCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAAt                                                                                                                                                                                             >  2:80626/1‑251 (MQ=255)                                                              aCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTg                                                                                                                                                                                     <  1:456441/251‑1 (MQ=255)                                                                   gCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTTAATGCATCCGGCTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACAGCGACCACTTTCATCATCGGCGCATCGACACCTTCTGGCGGGCAAATCTTCCCTGGGTTg                                                                                                                                                                                >  2:338746/1‑251 (MQ=255)                                                                    ccATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTg                                                                                                                                                                                >  1:421596/1‑250 (MQ=255)                                                                           cGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGtt                                                                                                                                                                        >  1:575775/1‑251 (MQ=255)                                                                            gCACCAGCGTTGCGCGCCCTTTCGGTGTATGGATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGTAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCCCTGGGGACCTGCCGATCGCATGTACTGAGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGACAAATCTTCCCTGGCTTGGGCCGGttt                                                                                                                                                                       >  2:433645/1‑250 (MQ=255)                                                                                 aGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCgg                                                                                                                                                                  <  1:194106/251‑1 (MQ=255)                                                                                   cGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGt                                                                                                                                                                <  1:229715/251‑1 (MQ=255)                                                                                         gcgcCCTTTCGGTGAATGAATCCGGTTCTGGTTGATCTTCATCGACGGACACATCGGACTACGGGCAGCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGATTCGGTTAGACGTGTCAAATg                                                                                                                                                          <  1:440050/251‑1 (MQ=255)                                                                                         gcgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATg                                                                                                                                                          <  2:79946/251‑1 (MQ=255)                                                                                                            atCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTgcgc                                                                                                                                         >  1:144910/1‑249 (MQ=255)                                                                                                              ccGGTTCTGGGCGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGCTGAGTCGGGTATGCGGGTCAAATGCCGCTTTCACTTTTCGCAGtt                                                                                                                                     >  2:569302/1‑251 (MQ=255)                                                                                                               gggTTCTGGGTGATATTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTc                                                                                                                                    <  2:6177/250‑1 (MQ=255)                                                                                                                ggTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCAGCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCt                                                                                                                                   <  2:405278/251‑1 (MQ=255)                                                                                                                           aTCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTc                                                                                                                        >  1:270974/1‑251 (MQ=255)                                                                                                                               tCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTcc                                                                                                                       <  2:443439/248‑1 (MQ=255)                                                                                                                               tCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTc                                                                                                                        <  1:339819/247‑1 (MQ=255)                                                                                                                               tCATCGACGGACACATCGGACTACGGACATCAACGTTGAAGCATAAACCGTTGCCGTTACAATCCATCGAACCACGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGCTCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCCACTCTTCCCTGGGTTGAGTCGGTTTTTCGGTTCAACTGCCGCTTTCACATTGCGCAGTTCTGCaaaaatgtcctcc                                                                                                                    >  2:322700/1‑243 (MQ=255)                                                                                                                                   cGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCg                                                                                                                 <  1:231108/250‑1 (MQ=255)                                                                                                                                        gACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAAc                                                                                                           <  1:536651/251‑1 (MQ=255)                                                                                                                                         acacATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACg                                                                                                          <  1:319708/251‑1 (MQ=255)                                                                                                                                                     aCGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTAt                                                                                               <  1:189791/250‑1 (MQ=255)                                                                                                                                                                aGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCCAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGaaa                                                                                    >  1:232210/1‑250 (MQ=255)                                                                                                                                                                       gCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGCGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGGCAAATGCCACTTTCACTTTGCGCAGTTCTGCAAAAAGGTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCtttcc                                                                            >  1:346117/1‑249 (MQ=255)                                                                                                                                                                         aTAAACCCTCGCTGTTACACTCCATCTCACCGCGCCACTGCTGGCGTACCTCAATGGTGATCTGCCGATCGAATGTACTGCGCTTACCCGCGTCCACTTTCATCATCGGCGCATCGAGACCGTCTTGCGGGCAAATCTTCCCTGGGTTGAGTCTGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTAAGCGCGAAAACCTTTGCCg                                                                          <  2:374742/251‑1 (MQ=255)                                                                                                                                                                          tAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCgc                                                                         >  2:504793/1‑250 (MQ=255)                                                                                                                                                                             aCCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTTCCGTGcc                                                                      >  1:467567/1‑250 (MQ=255)                                                                                                                                                                                gTTGCCGTTCCACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGc                                                                   <  2:28871/251‑1 (MQ=255)                                                                                                                                                                                gTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGCTCTGCCGATCGAATGTACTGCGCTTCACCGCGACCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGCTCAAATGCCGCTTTTACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAACGCGAAAACCTTTGCCGTGCTCGc                                                                   >  1:27419/1‑251 (MQ=255)                                                                                                                                                                                gTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCCCTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGGATTCAGCGCGAAAACCTTTGCCGGGCTCGc                                                                   >  2:268538/1‑251 (MQ=255)                                                                                                                                                                                   gCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGccc                                                                 <  2:120278/250‑1 (MQ=255)                                                                                                                                                                                        tACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCacaa                                                            <  1:566262/250‑1 (MQ=255)                                                                                                                                                                                                    cACCGCGCCACTGCTGGCTGACCTCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCTACTTTCATCATCGGCGCATCGAGACCTTCTGGCGTGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTGCCTCACCTAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCTCCGTAt                                               <  2:358929/251‑1 (MQ=255)                                                                                                                                                                                                        gcgcCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCg                                           <  1:269317/251‑1 (MQ=255)                                                                                                                                                                                                           ccACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCCCTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGc                                          >  2:54806/1‑249 (MQ=255)                                                                                                                                                                                                            cACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCCCAACCAACCACCGTATTTCGCAGt                                       >  2:316929/1‑251 (MQ=255)                                                                                                                                                                                                             cctgctgGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGGATTTCGCAGTc                                      <  2:493496/250‑1 (MQ=255)                                                                                                                                                                                                                 ctgGCGTACCGCAATGGGGATCTGCCGATCGAATGTAATGCGCTTCACCGCGTCCACTTTCCTCATCGGCGCATCGAGACCTTCTCGCGGGCAAATCATCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCcgcg                                  >  1:523164/1‑251 (MQ=255)                                                                                                                                                                                                                   ggCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCCGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCCCGCCCCCCAACAAACCACCGTATTTCCCAGTCAgcccc                                >  2:118307/1‑248 (MQ=255)                                                                                                                                                                                                                     cGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCCAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAATTGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCCGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGGATTTCGCAGTCAGCGccac                              >  2:406369/1‑251 (MQ=255)                                                                                                                                                                                                                       tACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGccacc                             >  1:358335/1‑250 (MQ=255)                                                                                                                                                                                                                        aCCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGccacca                            <  2:132677/250‑1 (MQ=255)                                                                                                                                                                                                                          cGCAATGGCGATATGCCGATCGCATATACTGCCCTTCACCGCGTCCACTTTCATCATCCGCGCATAGAGACCTTCTGGAGGGCAAATCTTCCCTGGGATGCGTCGGTTATGGGGGTCCAATGCCGCTTTCACTTTGCGCAATTCTGCAAACAGTTCCTTCCCCAAAAACCCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACCAACCACCGTATTTCGCAGTCAGCGCCACCACgc                         >  2:553525/1‑250 (MQ=255)                                                                                                                                                                                                                             aaTGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGtcat                      >  1:221934/1‑251 (MQ=255)                                                                                                                                                                                                                             aaTGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCGGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTAGTTCGCAGTCAGCGCCACCACGtcat                      <  2:247146/251‑1 (MQ=255)                                                                                                                                                                                                                                          cGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCtt         <  2:434626/251‑1 (MQ=255)                                                                                                                                                                                                                                              cGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTtcatc     <  2:575775/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aTGTACTGCGCTTCACCGCGTCCACTTTCATCAACGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCaaa  <  1:252070/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACCGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAA  >  NC_000913/1766768‑1767258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑