Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,748,841	C→T	R240K (AGA→AAA)	ydhT ←	uncharacterized protein YdhT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,748,841	0	C	T	100.0%	266.7 / NA	71	R240K (AGA→AAA)	ydhT	uncharacterized protein YdhT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/38);  total (33/38)

CGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAAT  >  NC_000913/1748596‑1749082                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  cGTTCGGCGCGTTGCCCTGGTTGCTGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCg                                                                                                                                                                                                                                              >  1:224152/1‑251 (MQ=255)      ggCGCGTTGCCCTGGTTGATGCACGGCCAGCCTTGCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTc                                                                                                                                                                                                                                         <  1:328272/251‑1 (MQ=255)         gcgTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATg                                                                                                                                                                                                                                      <  1:574527/251‑1 (MQ=255)                ccTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAAAAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGCACTTTTTCGCCGTCATTATATGcc                                                                                                                                                                                                                               >  2:466484/1‑251 (MQ=255)                       gATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTgcgg                                                                                                                                                                                                                        >  2:184584/1‑251 (MQ=255)                       gATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAAATGATGCTGTTCATTTAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGGgcgg                                                                                                                                                                                                                        >  1:406666/1‑251 (MQ=255)                             cgagaaGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGTCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGGGGACTCCGGCTCGAAAATTGTTTTCACCTTTAATGGGGACGGCGAAGCGAACTTTTTCGCCGGCATGATATGCCAGTGCGGGGCttt                                                                                                                                                                                                                  >  2:5902/6‑250 (MQ=255)                               aCCAGCCTTTCGTTCAGGGACTTACGTCATGTGCAGAAATTGGTGAGGTGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTGCGTTTGATTAAAGTTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCGCGAATATTGTTGTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATAGGCCAGTGCGGCGCTtcct                                                                                                                                                                                                                <  2:356622/251‑1 (MQ=255)                               aCCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTtcct                                                                                                                                                                                                                <  1:555710/251‑1 (MQ=255)                                ccAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCCTCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTtcctc                                                                                                                                                                                                               >  1:521392/1‑251 (MQ=255)                                       ttCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCGTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGcc                                                                                                                                                                                                         <  2:292926/250‑1 (MQ=255)                                         cGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCg                                                                                                                                                                                                       <  2:307244/250‑1 (MQ=255)                                             cAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGAAGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGaat                                                                                                                                                                                                  <  2:393461/251‑1 (MQ=255)                                               gggACTTACGGCATGGGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGGTTGATTAAAGGTAAAGCTATCAGCACTCCACAAACTGATTCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATTCCAGTGCGGCGCTTCCTCCTGGCCCGAGaata                                                                                                                                                                                                 >  2:316971/1‑250 (MQ=255)                                                      aCGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTc                                                                                                                                                                                         <  1:295463/251‑1 (MQ=255)                                                       cGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCg                                                                                                                                                                                        >  1:261213/1‑251 (MQ=255)                                                        ggCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGTGAATAATTGAGTCg                                                                                                                                                                                        >  2:170320/1‑250 (MQ=255)                                                          cATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGAcc                                                                                                                                                                                     <  2:422066/251‑1 (MQ=255)                                                              tgCAGAAATTGGTGATGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGGCGGCGTCTTGCGTTTGATTAAAGGTAAAGCTATCATCACTCAACAAACTGATGCTGTTCATTAAGTCGATAGCGGACTCCGGCTCGAATATTGTTTTCACCGTCAATGGCGACGGCCAAGCCAACTTTGTCGCCGTCATGAGATGCCAGTGCGGCCCGTCCTCACGGCCCGAAAATAATagaggcaacaacg                                                                                                                                                                                  <  2:356380/250‑10 (MQ=255)                                                                cAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGATCTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGccc                                                                                                                                                                               <  1:67013/251‑1 (MQ=255)                                                                     aTTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCt                                                                                                                                                                          <  1:526277/251‑1 (MQ=255)                                                                                   ggCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAAt                                                                                                                                                            <  1:302434/251‑1 (MQ=255)                                                                                        gggCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCa                                                                                                                                                       <  1:414635/251‑1 (MQ=255)                                                                                         ggCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCAc                                                                                                                                                      <  2:484508/251‑1 (MQ=255)                                                                                          gCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAACCTGATGCTGTTCATTAAGCCGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCAcc                                                                                                                                                     <  2:523839/251‑1 (MQ=255)                                                                                          gCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCAcc                                                                                                                                                     <  1:231762/251‑1 (MQ=255)                                                                                             gTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCt                                                                                                                                                  <  1:213215/251‑1 (MQ=255)                                                                                               cGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTgg                                                                                                                                                <  1:449007/251‑1 (MQ=255)                                                                                                  aaaGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCaa                                                                                                                                             <  1:209515/251‑1 (MQ=255)                                                                                                      ccTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTTTTCATTAAGACGATAGCGGACTCCTGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatgat                                                                                                                                         <  2:26662/251‑1 (MQ=255)                                                                                                         gCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCCTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatgatg                                                                                                                                        <  2:293574/249‑1 (MQ=255)                                                                                                             cccGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACa                                                                                                                                  <  1:358010/251‑1 (MQ=255)                                                                                                              ccGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTTCTCCCGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCTCTGGCAATGATGTGTACAt                                                                                                                                 <  2:277399/251‑1 (MQ=255)                                                                                                              ccGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACAt                                                                                                                                 >  1:166141/1‑251 (MQ=255)                                                                                                              ccGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACAt                                                                                                                                 <  1:8591/251‑1 (MQ=255)                                                                                                               cGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACAtt                                                                                                                                >  1:256976/1‑251 (MQ=255)                                                                                                                     cgtcgGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTcg                                                                                                                            <  1:5460/249‑1 (MQ=255)                                                                                                                            gTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCAGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTg                                                                                                                   >  2:282515/1‑251 (MQ=255)                                                                                                                                 tCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAAt                                                                                                              >  2:197079/1‑251 (MQ=255)                                                                                                                                   gTTTGATTAAAGGTAAAGCTATCAGCACTCAACAACCTGCTGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCCATGCTGTGCACATTGTCGCCGCTGTTGGCAATTc                                                                                                            >  2:322808/1‑251 (MQ=255)                                                                                                                                       gATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTgg                                                                                                         <  1:101197/250‑1 (MQ=255)                                                                                                                                           aaaGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGtt                                                                                                     <  1:318828/250‑1 (MQ=255)                                                                                                                                            aaGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTg                                                                                                   >  1:518716/1‑251 (MQ=255)                                                                                                                                            aaGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTg                                                                                                   >  1:198645/1‑251 (MQ=255)                                                                                                                                               gTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGACCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTgcgc                                                                                                >  2:78313/1‑251 (MQ=255)                                                                                                                                                 aaaGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACAGTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGcc                                                                                               <  1:67595/250‑1 (MQ=255)                                                                                                                                                    gCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAg                                                                                           <  2:28861/251‑1 (MQ=255)                                                                                                                                                     cTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGc                                                                                          >  2:99106/1‑251 (MQ=255)                                                                                                                                                      tATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCt                                                                                         >  1:49898/1‑251 (MQ=255)                                                                                                                                                       aTCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTTGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCt                                                                                         <  1:579741/250‑1 (MQ=255)                                                                                                                                                       aTCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTg                                                                                        >  1:164648/1‑251 (MQ=255)                                                                                                                                                                caacaaACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCGGGCGAttt                                                                                 <  2:467209/249‑1 (MQ=255)                                                                                                                                                                  acaaACTGCTGCTGTTCATTAACACGCTAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGACTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCAc                                                                             >  2:540880/1‑251 (MQ=255)                                                                                                                                                                               gTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCAtt                                                                 <  2:261213/250‑1 (MQ=255)                                                                                                                                                                                     tAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCg                                                          >  2:256193/1‑251 (MQ=255)                                                                                                                                                                                        gACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGACGCGAACTTTTTCTCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGCGAATAATTGAGTCGACCACGCCCGTGCTCACTCCCGTTTAATTTTCACCGCTGGCCATGATGTGTACATTGTCTCCGCTTTTGGCACTTCTTGCTTTTGCGCCCAATCTTGCGATTTTCACCAGTTCACCATTACtctcgtcc                                                       >  2:464376/1‑244 (MQ=255)                                                                                                                                                                                        gACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACa                                                       <  2:518716/251‑1 (MQ=255)                                                                                                                                                                                            aTAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGac                                                   <  1:274805/251‑1 (MQ=255)                                                                                                                                                                                              aGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGacac                                                 <  1:174339/251‑1 (MQ=255)                                                                                                                                                                                                 ggACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGt                                              >  2:297414/1‑251 (MQ=255)                                                                                                                                                                                                  gACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGtt                                             <  2:224152/251‑1 (MQ=255)                                                                                                                                                                                                          cTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCACAGCTGGCGATTTGCACCAGGTCACCATTCCTGTCGACATGACACCGTTCCCCCAAt                                     >  2:574993/1‑251 (MQ=255)                                                                                                                                                                                                               atatTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCAAGTGCGGCGCTTCCTCCTGGCCCGCGACTAATTGAGACGACCACGCCCGTGCTCACTACCGTTGCATTTTCACCGCTGGCAATGCTGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCACAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATCACACCGTTCACCCAATgaac                                 >  2:338857/1‑247 (MQ=255)                                                                                                                                                                                                                  ttGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAAc                             >  1:438197/1‑251 (MQ=255)                                                                                                                                                                                                                   tGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAAc                             <  1:6404/250‑1 (MQ=255)                                                                                                                                                                                                                         cACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACt                      >  1:439384/1‑251 (MQ=255)                                                                                                                                                                                                                             ttCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGCTTTGCACCAGGTCACCCTTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGc                   >  1:9599/1‑250 (MQ=255)                                                                                                                                                                                                                                  tGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCg             >  1:515687/1‑251 (MQ=255)                                                                                                                                                                                                                                      gACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTAt         >  1:581094/1‑251 (MQ=255)                                                                                                                                                                                                                                        cGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGCACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTc       >  2:404329/1‑251 (MQ=255)                                                                                                                                                                                                                                             aagcgaaCTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAAt  >  1:354090/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  CGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAAT  >  NC_000913/1748596‑1749082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑