| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,358,859 | T→C | Y110C (TAT→TGT) | puuP ← | putrescine:H(+) symporter PuuP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,358,859 | 0 | T | C | 91.2% | 183.9 / ‑4.2 | 68 | Y110C (TAT→TGT) | puuP | putrescine:H(+) symporter PuuP |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (32/30); minor base A (2/4); total (34/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGATAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCA > NC_000913/1358613‑1359105 | cAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGTAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGGTAATCATCGGCAAAAA‑GAGACAATc < 2:310671/251‑1 (MQ=255)cAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCCCACCC‑ACGGCGGCACTTCCGGGGAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCCAAAA‑GAGACAATc > 1:285786/1‑251 (MQ=255) aGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTTAGATTCGCGGCGGTTAAAATGGCGCCGAAGGGTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGGGA‑TAGATTTTCGCCCACAAGACGTTAATCATCGGCCAAAA‑GAGACAATcc > 2:393729/1‑251 (MQ=255) ccAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑CCGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑AAGACAATCCAGCcgcgc > 2:523/1‑250 (MQ=255) aaCGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGGCGAAGGGTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGGGGGGGGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATg > 2:391830/1‑251 (MQ=255) aaCGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATg < 1:437009/251‑1 (MQ=255) cAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCACAAATc > 1:308564/1‑251 (MQ=255) aGTCCCTGAACGACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATcc < 2:134812/251‑1 (MQ=255) ccTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACg < 2:449451/251‑1 (MQ=255) tGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTg > 1:414603/1‑251 (MQ=255) cAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTAAAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAACACCCACACCC‑ACGGCGGCCCTTTCGGGAAGAGGGCGGAGAGA‑TAGATTTTTGCCAACAAGACGGTAATCATCGGCAAAAA‑GAGACAAACCAGCAGCGAGGACCA‑GACGA‑CCATAAACCCGACGTGCGGGTTa > 1:253155/1‑250 (MQ=255) ggAAGATAAAAACCATCATGATGGAGATTTCCCCCAGTAAAAACAGGGCATTGAAGTTAACGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTTAAATGGCGACGAAGGGTACCACCCACACCC‑CCGGCGGCACTTCCGGGGAGAGGGCGGGGAGG‑TAGATTTTCGCCAACAAAACGTTAATCATCGGCAAAAA‑GAGACACTCCCACAGCGCTGACCA‑CCCGA‑CCATAAATCCGACGTGCGGGGTAAtt > 2:353446/1‑250 (MQ=255) aTAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGAACAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGCAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGGGGAGAGA‑TAGATTTTCGCCCACAAGACGTTAATCATCGGCAAAAA‑GAGACAAACCAGCAGCGATGACCA‑GCCGA‑CCATAAAACCCACGGTCGGGTTAATCGACtt > 2:535834/1‑251 (MQ=255) aaCACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAAGCCAGCAGCGATGACCA‑GCCGA‑CCATAAACCCGACGTGCGGGTTAATCGACTTTTg < 1:52599/251‑1 (MQ=255) accaTGGTGGAGATTTGCACCAGAACAAACAGGGACTTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTAGAGGCGGTTAAAATGGCGAAGAACGGTACCACCC‑CACCCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGA‑TAGATTTTTGCCAACAAAACGTTAATCATCGGCAACAA‑GAGACAATCCAGCTGCGCAGACCA‑GCCGC‑CCCTAAATCCgccgggccgggtattcgaat > 2:528721/1‑222 (MQ=255) accaTGATGGAGATTTGCACCAGTACAAACAGGGGATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTGCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGTCGTTAATCATCGGCAAAAA‑GAGTCAATCCAGCAGCGATGAGCA‑GTCGA‑CCATAAATCCGACGTGCGGGTTAATCGACt < 1:528721/241‑1 (MQ=255) caTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCCCACCC‑CCGGCGGCACTTCCGGGGAGAGGGCGGGGAGA‑TAAATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAAACCAGCAACGGTGACAA‑ACCGAACCA‑AAATCCTAAGCGCGGGTTAAACGACTTTTG‑CGCGGAgg > 2:453382/1‑251 (MQ=255) caTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGATTAAAATGGCGACGAAGGGTACCACCCACACCC‑ACGGCGGCACTTCCGGGGAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAAACCAGGAGCGATGACCC‑GCCGA‑CCATAAATCCGACGTGCGGGTTAAACGACCTTTG‑CGCGGAgg > 2:289615/1‑251 (MQ=255) atgatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGGTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGATTGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGTCGTGCGGGTTAAGCGACTTTTG‑CGCGTAGGt < 1:516390/251‑1 (MQ=255) gatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAAATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGAAAATCCAGCAGCGATGACCA‑ACCGA‑CCCTAAATCCGACGTGCGGGGTAATCGACTTTTG‑CGCGGAGGtat > 1:478313/1‑251 (MQ=255) tGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAAcc > 1:258596/1‑251 (MQ=255) aCCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCATCAGATTCGCGGCGGTTAAAATGGCGCCGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTGCTCCAACAAGACGTTTATCATCGGCAAAAA‑GAGACTATCCAGCATCGACGACCA‑GCCGA‑CCTTTAATCCGACTTGCGGGTTAATCTACGTTTG‑CGCGTAGGTATAGGCCGAACCGGc < 1:336980/251‑1 (MQ=255) aCAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCgg > 1:13747/1‑251 (MQ=255) aCAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCg < 1:412339/250‑1 (MQ=255) cAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCC‑ACGGCGGCACTTCCGGGGAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTCATCATCGGCAAAAA‑GAGACCATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATACGACGTGCGGGGTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCggg > 2:186117/1‑250 (MQ=255) gTATTGAAGTTCGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTGAAATGGAGACGAAGGATAACACCCACACCC‑ACGGCGGCACCTCCGGGAAGAGGGCGGAGGGA‑TAGATTTTCGCCCACAAGACGTTAATAATCGGCAAAAA‑GAAAAAACCCAAAAGCGATGACAC‑GCCGA‑ACAAAAATCCGACGCGGAGGTTAATCAACTTTTG‑CGCGTAGGACAAGGGCGAACCCGCAACCGGAAACT‑GGCg > 2:298633/1‑251 (MQ=255) tGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTTGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGTAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAAc < 1:248240/250‑1 (MQ=255) tAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGGCGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGttt < 1:391830/250‑1 (MQ=255) tAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGttt < 2:229727/250‑1 (MQ=255) gACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGt < 1:583345/251‑1 (MQ=255) aCCAGGTTGACGCTCTTCAGATTCGCGGCGGTTTACATGGCGACGAAGGGTACCCCCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGGGAGA‑TAGAATTTCGCCAACACAACGTTAATCATCGGCAAAAA‑GAGACAATCCCGCAGCGATGACCA‑GCCGC‑CCATAAATCCCACGTGCGGGGTAATCGACTTTTT‑CGCGGAGGTATAAGCCGAAACGGCCTCCGGAAAAC‑GGCGAAACAGGTTGCCgg > 2:584273/1‑249 (MQ=255) tGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGAt < 1:208583/250‑1 (MQ=255) cGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCg < 1:290635/251‑1 (MQ=255) gCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCgg > 1:63331/1‑251 (MQ=255) cagatgggcggcgGCTAAAAGGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACGTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTGAAGCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAAACGACTTTTG‑CGCGGAGGTAGAGGCCGAACGGGCCTCCGGAGACT‑GGCGAACCAGTTTGCCGTAACTGAGAGCTGTAAACa < 2:63440/244‑1 (MQ=255) aGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAg < 1:137903/251‑1 (MQ=255) cgcgGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGcaca < 1:469571/250‑1 (MQ=255) ggcggTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcg > 2:243878/1‑251 (MQ=255) tAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccagcg > 2:190120/1‑251 (MQ=255) gTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGc > 1:163478/1‑251 (MQ=255) ttACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGcc > 1:207472/1‑251 (MQ=255) tACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCg > 2:435047/1‑251 (MQ=255) cacccacaccc‑aCGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGa > 2:387338/1‑250 (MQ=255) ccacaccc‑acGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCAGCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGCCGTGCGGGTTAATCGATTTTTG‑CGGGTAGGTATAGGCCGACCCGGCCTCCGGAACCT‑GGCGAACCAGTTGGCCGTAGCTAATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGt < 1:316357/251‑1 (MQ=255) cacaccc‑acGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTg > 1:206561/1‑251 (MQ=255) gggcACTTCCGGGAAGAGGGGGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTGGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTaa < 1:393729/250‑1 (MQ=255) gcACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATg < 1:81477/251‑1 (MQ=255) aCTTCCGGGAAGAGGGCGGA‑AGAATAGATTTTCGCAAAAAAGACGTTAATCCTCGGCAAAAA‑GAGAAAATCCAGAAGCGATGACCA‑GCCGA‑CCATAAATCCGACGGGCGGGTTAATCGACTTTGG‑CACGTAGGTATAGGCCGAACCGGCCTCCGGAAACG‑GGCGACCCAGTTTGCCGTAGCTGATAGGGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGcc < 2:398945/251‑1 (MQ=255) tCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCg < 1:293655/249‑1 (MQ=255) cggggAAGGGGGAGGAGAAA‑TGAATTTTCACCAACAAGACTTTAAACATCGGAAAAAA‑GAGACAACCAAGCGGCGATGACCA‑GCCGA‑CCAAAAATCCGACGTGCGGTTTAATCGACTTTTG‑CGCGTGGGTATAGGCCGAACCGGCCCCCGGAAACG‑GGCGACCCAGTTTGCCGTAGCTGAGAGCGGTAAACAGCACGCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGgag < 2:123468/249‑1 (MQ=255) agagGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAAt > 2:146058/1‑250 (MQ=255) gggggaggAGAGA‑TAGATTTTCGCCAAAAACAGGTTAA‑CAACCGCAAAAAAGGGACAACCGAGCA‑CGGTGGCCAAGCAGA‑CCAAAAACCCGCCGTGGGGGTTAATCGACTTTTG‑CGCGTAGTTATAGCCCAACCCGGCCTCCGGAACTT‑GGCGAACCATTTTGCCGTAGCTGATGGGGGTAAACAGCCCCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATAc < 2:320193/245‑1 (MQ=255) ggCGGAGAGA‑TAGATTTACGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAACCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCCCCGGAAACT‑GGCGACCCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCaa < 1:243878/251‑1 (MQ=255) gggAGAGA‑TAGATTTCCGCCAACAGGACGTTAATCATCGGCAAAAA‑AAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACG‑GGCGAACCAGTTGGCCGTAGCTGATAGCGGTAAACAGCCCACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCaaaa < 1:453382/250‑1 (MQ=255) ggaga‑TAGATTTCCCCCAACAAGACGTAAATCATCGGAAAAAA‑GAGACAACCCAGCAGCGATGACCA‑GCCGA‑CCAAAAACCCGACGTGCGGGTTAATCGATTTTTG‑CGCGTGGGTATAGGCCGACCCGGCCTCCGAAAACT‑GGCGACCCAGTTGGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTa < 2:414603/250‑1 (MQ=255) aagtttttCGCCAAAAAGACGTAAATCTTCGTAAAAAA‑GAGAAAACCCAGCAGCGATGACAA‑GCCGA‑CCAAAAACCCGCCGGGCGGTTTAATCG‑CTTTTGGCGGGTAGGTATAGGCCAACCCGGCCTCCG‑AAACTAGGCGAACAAGTTTGCGGTAGCGGATAGCGTAAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCaaa < 2:308564/247‑1 (MQ=255) tttCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑AGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACATTACAAAAAGTATCAAATACgg > 1:258574/1‑251 (MQ=255) caaacaaGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATc < 1:9661/249‑1 (MQ=255) acaaGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGc > 1:376387/1‑251 (MQ=255) aaGACGTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACACGACAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGt > 2:15237/1‑251 (MQ=255) gTTAATCATCGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTg > 2:123853/1‑247 (MQ=255) aaTCATCGGAAAAAA‑GAGACAAGCCGGCAGCGATGACCA‑GCCGC‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGATATAgg < 1:128302/251‑1 (MQ=255) catcGGAAAAAA‑GAGAAAATCCAGCAGCGAGGACCA‑GCCGA‑CCATAAATCCGACGGGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACG‑GGCGAACCAGTTGGCCGTAGCTAATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGcc < 1:276272/250‑1 (MQ=255) tcGGCAAAAA‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGcc > 1:421234/1‑248 (MQ=255) aaaaa‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTGGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGAAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCcat < 1:179731/251‑1 (MQ=255) aaa‑GAGACAATCCAGCAGCGATGACCA‑GCCGA‑CCCTAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCcatc > 1:236544/1‑250 (MQ=255) a‑gagAAAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAACCCGACGTGCGGGTTAATCGCCTTTTG‑CGCGTAGTTATAGGCCGACCCGGCCTCCGGAAACG‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCCCACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCatcatc < 1:540974/251‑1 (MQ=255) gagaCAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCatcatcc > 2:269636/1‑250 (MQ=255) | CAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGA‑TAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAA‑GAGATAATCCAGCAGCGATGACCA‑GCCGA‑CCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACT‑GGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCA > NC_000913/1358613‑1359105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |