Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,290,670	G→A	W143* (TGG→TGA)	rssB →	regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,290,670	0	G	A	100.0%	92.5 / NA	27	W143* (TGG→TGA)	rssB	regulator of RpoS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/7);  total (20/7)

CACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAAC  >  NC_000913/1290426‑1290913                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    cACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCa                                                                                                                                                                                                                                               <  2:419000/251‑1 (MQ=255)      aTGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGt                                                                                                                                                                                                                                          <  1:351191/251‑1 (MQ=255)         aaCGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAAGTCGCGGGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGa                                                                                                                                                                                                                                       <  2:74476/251‑1 (MQ=255)               cTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAAccc                                                                                                                                                                                                                                 >  2:154875/1‑251 (MQ=255)                    aCTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATATCCCTGCCg                                                                                                                                                                                                                            <  2:35981/251‑1 (MQ=255)                                tataCGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAat                                                                                                                                                                                                                >  1:358853/1‑251 (MQ=255)                                          agagGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAgg                                                                                                                                                                                                       >  1:3055/1‑250 (MQ=255)                                          agagGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAgg                                                                                                                                                                                                       >  2:207734/1‑250 (MQ=255)                                                   cAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAAcc                                                                                                                                                                                             <  1:62115/251‑1 (MQ=255)                                                        cccAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGACTCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCgg                                                                                                                                                                                        >  2:263024/1‑251 (MQ=255)                                                                                 gAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCg                                                                                                                                                               >  2:189756/1‑251 (MQ=255)                                                                                  aaaaTATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCgg                                                                                                                                                              >  2:216181/1‑251 (MQ=255)                                                                                   aaaTATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCggg                                                                                                                                                             >  2:415733/1‑251 (MQ=255)                                                                                     atatGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGtt                                                                                                                                                           >  1:71542/1‑251 (MQ=255)                                                                                             gATATTGCCAAAGCATTACGTCTGGGCGTTGAAGATGTTTTACTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTCTTACAGGAACTACACCCGCCGGTTCAGCAGGTGATTTCGCATTGCCGGGTTAATTATCg                                                                                                                                                   >  2:239416/1‑251 (MQ=255)                                                                                              atattgaaaaaGCTTTCCGTGTGGGGGTTGAATATTTTTTGCTAAAACCATTAAAATATCTAAGTCGGTTGCGGGAG‑TGGTTTTTCCCCTGTCTCTACCCCAGAATTTTTATTCCGCGGGTGGAGAAAGAGGAAAGTTTTTTTCGCGACTGAGATGCATTGTTTAAAACCCCTGCCGCGGCGGAAAATTTATTAAAGAAACAACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGt                                                                                                                                                  <  2:90271/243‑1 (MQ=255)                                                                                                                                    ttGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTg                                                                                                             >  1:573345/1‑250 (MQ=255)                                                                                                                                     tgctaaaaccataaaaaGATCTGAGTCGGTGGCGCGAGATGTTTTTTGCT‑TTTTTCTACCCCATATTGTTTAATTCGCGGGTTGAGGAAGGAGAAAGGTTTTTCCGCGAGTGAGATGCATTGTTAGATACCCCTGCCGCGGCGGCAAAATTTTTACAGAAACAACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCtcga                                                                                                            <  1:445369/236‑4 (MQ=255)                                                                                                                                             ccAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGc                                                                                                   >  2:105956/1‑251 (MQ=255)                                                                                                                                                         cTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTAGGaaa                                                                                        >  2:563292/1‑250 (MQ=255)                                                                                                                                                                              gTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAACGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAACACCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCt                                                                  >  2:302390/1‑251 (MQ=255)                                                                                                                                                                                 tttGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTg                                                                >  1:259295/1‑250 (MQ=255)                                                                                                                                                                                         gTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCAcc                                                        >  2:135839/1‑251 (MQ=255)                                                                                                                                                                                             ctATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCg                                                      >  1:503693/1‑249 (MQ=255)                                                                                                                                                                                                                             aagaggaaAGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTCCAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGttt                    >  2:285580/1‑250 (MQ=255)                                                                                                                                                                                                                                   aaaGGCTTTTTCGCGACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTAcgc              >  2:122970/1‑251 (MQ=255)                                                                                                                                                                                                                                               gcgACTGAGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAAc  >  2:458763/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    CACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCC‑TGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAAC  >  NC_000913/1290426‑1290913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑