Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,147,792	T→A	S58T (TCG→ACG)	plsX →	putative phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,147,792	0	T	A	100.0%	175.2 / NA	50	S58T (TCG→ACG)	plsX	putative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (31/19);  total (31/19)

GCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAA  >  NC_000913/1147545‑1148035                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    gCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTAcgc                                                                                                                                                                                                                                                  >  1:139165/1‑251 (MQ=255)       gCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGt                                                                                                                                                                                                                                                <  1:58089/247‑1 (MQ=255)         gTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAg                                                                                                                                                                                                                                          <  1:300973/251‑1 (MQ=255)                          ggATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGcagtc                                                                                                                                                                                                                         <  1:4131/251‑1 (MQ=255)                                    cAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGCCACGTCTAACCCTGGCGTTAGGTGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACACCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGcc                                                                                                                                                                                                               >  1:48173/1‑251 (MQ=255)                                     aGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCa                                                                                                                                                                                                              <  2:303984/251‑1 (MQ=255)                                          aCTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGAt                                                                                                                                                                                                         >  2:12055/1‑251 (MQ=255)                                             ggggAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGcc                                                                                                                                                                                                      <  1:515371/251‑1 (MQ=255)                                                gAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCgg                                                                                                                                                                                                   <  1:444733/251‑1 (MQ=255)                                                                cggcgACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATcc                                                                                                                                                                                   >  2:118802/1‑251 (MQ=255)                                                                    gacgaTACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCgcgc                                                                                                                                                                               >  2:167903/1‑251 (MQ=255)                                                                       gaTACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGcc                                                                                                                                                                              >  2:452685/1‑249 (MQ=255)                                                                       gaTACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGcc                                                                                                                                                                              >  1:409047/1‑249 (MQ=255)                                                                               gACACGTCTACCCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGa                                                                                                                                                                    <  2:36019/251‑1 (MQ=255)                                                                                        aaCCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATgc                                                                                                                                                           >  2:459829/1‑251 (MQ=255)                                                                                                              ggAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGa                                                                                                                                      >  1:218780/1‑250 (MQ=255)                                                                                                                aGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGACATCACGCCATTACTTGATAAAGCTGACTTTGAACAACGTACGCGTATGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGACGTGGGAGTTCAATGCGCGTGGCCATGGAGCTGGTGaacg                                                                                                                                   >  1:321368/1‑249 (MQ=255)                                                                                                                aGGGGATTTTGGACCTTCCGAGACAGTGTCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGATGTGGGAGTTCAATCCGCGTGGCCCTGGAGCTGGTGaacg                                                                                                                                   >  1:321357/1‑249 (MQ=255)                                                                                                                   ggATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTACTTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAaaga                                                                                                                                  >  2:559452/1‑249 (MQ=255)                                                                                                                           gCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCTCTTAATTCTAATTCGCAACTCACTCTTCTTTTAGGCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGTCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTTAAAGAAGGTCGAgcg                                                                                                                        <  2:244820/251‑1 (MQ=255)                                                                                                                                       cAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTc                                                                                                            >  1:537432/1‑251 (MQ=255)                                                                                                                                        aGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTc                                                                                                            >  1:349517/1‑250 (MQ=255)                                                                                                                                           gCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTg                                                                                                        <  1:130468/251‑1 (MQ=255)                                                                                                                                                caccaTTGCAGGCACTGAATTCTATTTCGAAACTCACTCTTCTTTTAGTCGGCAATTCCGACCCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGt                                                                                                   <  2:148087/248‑1 (MQ=255)                                                                                                                                                   caTTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAAt                                                                                                >  1:455846/1‑251 (MQ=255)                                                                                                                                                    aTTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAAt                                                                                                >  1:404291/1‑250 (MQ=255)                                                                                                                                                        cAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCgg                                                                                           >  2:526078/1‑251 (MQ=255)                                                                                                                                                         aGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGACAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCGGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCggg                                                                                          <  2:31053/251‑1 (MQ=255)                                                                                                                                                          ggCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCgggg                                                                                         >  2:142759/1‑251 (MQ=255)                                                                                                                                                           gCACTAAATTCTCGTTCGCAACTCACTTTGCTTTAGGGCGGCAATGCCGCCGCCATCACGCCATTACTTGCTAAAGCTGACTTGGAACAACGTACGCGTCTGCAGATTATTCCTGCGCCGTCAGTTAGCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGgc                                                                                        <  2:321368/251‑1 (MQ=255)                                                                                                                                                            cACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGgcg                                                                                       >  1:223675/1‑251 (MQ=255)                                                                                                                                                             aCTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGgcgc                                                                                      >  1:32756/1‑251 (MQ=255)                                                                                                                                                                 aaTTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGAt                                                                                  >  2:25657/1‑251 (MQ=255)                                                                                                                                                                 aaTTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGAt                                                                                  <  1:559452/251‑1 (MQ=255)                                                                                                                                                                     cTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATgggg                                                                              >  1:149614/1‑251 (MQ=255)                                                                                                                                                                           cGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGc                                                                         >  2:281258/1‑250 (MQ=255)                                                                                                                                                                              aaCTCAATCTTCTATTAGTCGCCAATTCCGACGCCATCACGCCATTAATTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGCTTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCACTGCGCGCGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGATGCTGGGGCTGGCaaa                                                                      >  2:568214/1‑250 (MQ=255)                                                                                                                                                                                          ttttAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCa                                                           >  1:247152/1‑249 (MQ=255)                                                                                                                                                                                            ttAGTCGCAAATTCCGACGCAATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACTTACGCGTCTGCAGATTATTCCTGCGCAGTCATTTATCGCCAGTGATGCCCGGCCTTCGCAAGCATTCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCGGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGc                                                        <  2:312029/250‑1 (MQ=255)                                                                                                                                                                                                tCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTg                                                   >  1:191526/1‑251 (MQ=255)                                                                                                                                                                                                        cccGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACTTACGCTTCTGCATATTATTCCTGCGCAGTCAGTTATCGCCATTGATGGCCGTCCTTCGCAAGCTATCCGCGCCAGGCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAATAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATTCGGGGGCGCGGTTAGGTCTGTCAAAATTTTTACTcgatccttttttgggga                                            <  2:236350/249‑17 (MQ=255)                                                                                                                                                                                                                 aTCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTcc                                  <  1:405339/251‑1 (MQ=255)                                                                                                                                                                                                                    aCGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGgc                               >  1:109494/1‑251 (MQ=255)                                                                                                                                                                                                                               tGCTAAAGCTGACTTTGACCAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGAc                      <  2:377845/249‑1 (MQ=255)                                                                                                                                                                                                                               tGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGAc                      <  1:12055/249‑1 (MQ=255)                                                                                                                                                                                                                                 cTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGt                   >  1:360283/1‑250 (MQ=255)                                                                                                                                                                                                                                         accTTTGAACAACGTACTCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGAGGCCCGTCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACTGTATTACcac           <  2:142098/248‑1 (MQ=255)                                                                                                                                                                                                                                                aacaacGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCa    >  1:515432/1‑250 (MQ=255)                                                                                                                                                                                                                                                  caacGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCaaa  >  2:442476/1‑250 (MQ=255)                                                                                                                                                                                                                                                   aacGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCaaa  >  2:70299/1‑249 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    GCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAA  >  NC_000913/1147545‑1148035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑