Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	769,388	A→T	I471F (ATC→TTC)	mngB →	alpha‑mannosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	769,388	0	A	T	100.0%	197.5 / NA	52	I471F (ATC→TTC)	mngB	alpha‑mannosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (22/30);  total (22/30)

TAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATATCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGGTAATTACCGCAGCGAACGCGAAACCGCAATGCGAT  >  NC_000913/769147‑769636                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          tAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTGCCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTGATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTGCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCg                                                                                                                                                                                                                                                   <  1:35119/250‑1 (MQ=255)               ggCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAAc                                                                                                                                                                                                                                    >  1:468322/1‑251 (MQ=255)                gCCGCGTGAAGAAGTTATCAACACCACTGGGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACg                                                                                                                                                                                                                                   <  2:170381/251‑1 (MQ=255)                    cgTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGAAACGTAAt                                                                                                                                                                                                                               <  2:538688/251‑1 (MQ=255)                    cgTGAAAAAGTTATCAACACCACCGTGCGGCTGCGCGCCATCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAAt                                                                                                                                                                                                                               <  2:504177/251‑1 (MQ=255)                     gTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAAtt                                                                                                                                                                                                                              <  1:119/251‑1 (MQ=255)                       gaagaaGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTg                                                                                                                                                                                                                             <  2:385888/250‑1 (MQ=255)                         agaagTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTGTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCgg                                                                                                                                                                                                                          <  2:374146/251‑1 (MQ=255)                               tATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCaaaa                                                                                                                                                                                                                     <  1:50429/250‑1 (MQ=255)                                         acTGTGCGGCTGAGAGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGCCGCTg                                                                                                                                                                                                           >  2:556769/1‑250 (MQ=255)                                            gtgCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGaa                                                                                                                                                                                                         <  2:137511/249‑1 (MQ=255)                                               cGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGAt                                                                                                                                                                                                    <  2:44063/251‑1 (MQ=255)                                                     cgcgcCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCTAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGa                                                                                                                                                                                              <  1:289382/251‑1 (MQ=255)                                                              cagTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCttt                                                                                                                                                                                     <  1:535713/251‑1 (MQ=255)                                                               agTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGAGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGCTATACAGATCAACCAGATTGTCACTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTc                                                                                                                                                                                    >  1:555863/1‑251 (MQ=255)                                                                         gCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTg                                                                                                                                                                          <  2:416546/251‑1 (MQ=255)                                                                         gCGGGACGATCGCGGTCAGCCTGTACCGGATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAAtt                                                                                                                                                                           <  2:427791/250‑1 (MQ=255)                                                                          cGGGACGATCGCGGTCAACCTGTACCGTATTTTCTTCGCCATGCCCGTGAGATCGATCCAGGCCTACTCGATCGGCAAATAGTTCATTACGGTAATTACGACCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTgc                                                                                                                                                                         >  2:274096/1‑251 (MQ=255)                                                                                gATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCa                                                                                                                                                                    >  2:470150/1‑250 (MQ=255)                                                                                   cgcgGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGa                                                                                                                                                                <  1:553394/251‑1 (MQ=255)                                                                                   cgcgGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGa                                                                                                                                                                <  2:473332/251‑1 (MQ=255)                                                                                      ggTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTACTTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAgg                                                                                                                                                              >  1:299349/1‑250 (MQ=255)                                                                                        tCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATg                                                                                                                                                           >  2:8804/1‑251 (MQ=255)                                                                                                               gCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATaa                                                                                                                                    >  1:183941/1‑250 (MQ=255)                                                                                                                ccATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAg                                                                                                                                  <  2:57226/251‑1 (MQ=255)                                                                                                                        gTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGgt                                                                                                                          >  1:464649/1‑251 (MQ=255)                                                                                                                          gagaTCGATCCAGGCCTAATCGATCGTAAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTCTGGGCTATCGCACGCTTTATTTCGTAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGCAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCggagt                                                                                                                        >  2:409091/1‑248 (MQ=255)                                                                                                                           agaTCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGtgtg                                                                                                                       <  1:13983/251‑1 (MQ=255)                                                                                                                            gatcgatcCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGGTGGTTCTCTGCAACTGGT‑AGATAAAGACATCGGTGTgc                                                                                                                      <  1:442528/251‑1 (MQ=255)                                                                                                                                 atcCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTAt                                                                                                                 <  1:141239/251‑1 (MQ=255)                                                                                                                                      ggCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCg                                                                                                            <  2:149992/251‑1 (MQ=255)                                                                                                                                             gcgatcgGCAAATAGTTCATTACGGGAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAAGCAGCCGGGCTACGTAATTGCGGCAAAAAGTGACGCTGAATGGATACTGTAAAATGCTGTCGGGCAAAGTTCGCTCAATGAGGATGGTGCGCGGCATC‑GGTGAGGTAAAGACAGCGGTGTGCGCTATGACCGGGTATTg                                                                                                     <  1:561716/250‑1 (MQ=255)                                                                                                                                                         tAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTgaaga                                                                                          >  2:79705/1‑250 (MQ=255)                                                                                                                                                             tccttACGGTAACTACGATCCCTTTATGGGGTTAGATATACAGATCACCCAGATTGTACCCTCTATGGGCTATCGCCCGATTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGATGACGGGATACTGGGAACTGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGAAAGAAGTGTGCGATATGACCGGGGATTGCAAATTGAAGAACGct                                                                                     >  2:283237/4‑251 (MQ=255)                                                                                                                                                              cATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGAATGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAgatc                                                                                    <  2:367393/251‑4 (MQ=255)                                                                                                                                                                ttACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCtg                                                                                  >  2:449057/1‑251 (MQ=255)                                                                                                                                                                 tACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCtg                                                                                  <  2:169519/250‑1 (MQ=255)                                                                                                                                                                 tACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCtg                                                                                  <  1:15631/250‑1 (MQ=255)                                                                                                                                                                       aaTTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATgg                                                                           <  1:556100/251‑1 (MQ=255)                                                                                                                                                                              aTCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATTCTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGtgatga                                                                     >  2:404838/1‑250 (MQ=255)                                                                                                                                                                              aTCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGtgatga                                                                     >  2:495692/1‑250 (MQ=255)                                                                                                                                                                                  cTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATg                                                                >  2:452074/1‑251 (MQ=255)                                                                                                                                                                                             ttGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCAcc                                                      <  1:315084/250‑1 (MQ=255)                                                                                                                                                                                                     cAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCaaa                                                >  2:262178/1‑248 (MQ=255)                                                                                                                                                                                                      aGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTTTGCGCTAGTACCGGGTATTGCAAATTGAAGAAAGCTCTGATGAGGGTGGTGAATATGACTATTCACCCGCAAaagaa                                            <  2:263614/251‑1 (MQ=255)                                                                                                                                                                                                                 aTTGTCCCTTCTAGGGGCTATCGCACGCTTTATTTCGAAGCGAATAAGCCTGGCACCGTAATTGCGGCAAAAAGTGACGCTGACGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGGTAAt                                 >  2:369200/1‑251 (MQ=255)                                                                                                                                                                                                                   tGTCCCTTCTATGGGCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGGTAAtt                                >  1:558446/1‑250 (MQ=255)                                                                                                                                                                                                                            tATGGGCTATCGCACGCTTTATTTCGAAGCGAATCCGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAACGAAGAGTGGGTAATTACCGCAGCg                       >  2:517222/1‑250 (MQ=255)                                                                                                                                                                                                                                 gCTATCGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGGTAATTACCGCAGCGAAcgcg                 >  1:534411/1‑251 (MQ=255)                                                                                                                                                                                                                                    aTCGCAAGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGAGGGTAATTACCGCAGCGAACGCGaa               >  2:388004/1‑250 (MQ=255)                                                                                                                                                                                                                                      cGCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGGTAATTACCGCAGCGAACGCGAAAc             <  1:250262/250‑1 (MQ=255)                                                                                                                                                                                                                                       gCACGCTTTATTTCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGGTAATTACCGCAGCGAACGCGAAACCg           <  1:441749/251‑1 (MQ=255)                                                                                                                                                                                                                                                atatCGACTCGAAGCAGCCTGGCAACGTAAGTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGAAGAATATGACTATTCACCCGCAAAAGAAGAGTGGGTAATTACCGCAGCGAACGCGAAACCGCAATGCGat  <  1:313664/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          TAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAAATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATTGTCCCTTCTATGGGCTATCGCACGCTTTATATCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGT‑AGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGGTAATTACCGCAGCGAACGCGAAACCGCAATGCGAT  >  NC_000913/769147‑769636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑