Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	81,102	G→A	S286L (TCG→TTG)	leuB ←	3‑isopropylmalate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	81,102	0	G	A	100.0%	163.4 / NA	51	S286L (TCG→TTG)	leuB	3‑isopropylmalate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/27);  total (24/27)

AGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCG  >  NC_000913/80859‑81343                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  aGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAgccgcc                                                                                                                                                                                                                                            >  1:323741/1‑251 (MQ=255)   ccATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGccgcccg                                                                                                                                                                                                                                          <  1:402542/251‑1 (MQ=255)      ttattacaccccgttggctcaaTAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCTTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCATTGCCAGCGAAAGGATTTGTG‑CAAGCGGGTTGGCGATTTTTTTGCCTTCGATA‑TCTGGTTCCAAGCcgcccgcc                                                                                                                                                                                                                                        <  2:559342/229‑1 (MQ=255)          aaccccccTTCTGCAAAATAGCGGACAAGGATATCGCCCATTTCATCGGTACAAACGGCGGCACCGCACCGGGCTAAACCCCCGGTGCAAAGGCCTTCTTCTAATGCGCGTTTAATGGCGCTTTAAAGGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCGGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATTTTTTTGCCTGCGATA‑TCGGGTGCCAAGCCGCCCGCCGGTTc                                                                                                                                                                                                                                   <  2:459402/247‑1 (MQ=255)              ccccTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAACAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGGGCCAAGCCGCCCGCCGGTTCATAc                                                                                                                                                                                                                               >  2:94664/1‑251 (MQ=255)               cccTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATAc                                                                                                                                                                                                                               >  1:261385/1‑250 (MQ=255)                         acagaGCGGGCAAGTATCTCGCCCATTTATTGGGTACTAACGGCGGAAGCGCCACGGGCTAAATCCCGGGTGCGAAGGCCTGTTCCAAAGGGGGGGTGAAGGGCGCGTT‑AATGGGCGCAAGCCGCATCATCGGCATCAAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAAcc                                                                                                                                                                                                                    <  2:628/247‑1 (MQ=255)                                                cATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGGGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATC‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGa                                                                                                                                                                                               >  1:470733/1‑249 (MQ=255)                                                     cATCGGTACAAGCGGCGGCAGCGCCCGGGGCTAAATCCCGGGTGCGAATGCCTTTTTCAAAGGCGCGTTTAAGGGCGGGTTAAATGG‑CCCAAGCCGAATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCAAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAAc                                                                                                                                                                                        <  2:220888/251‑1 (MQ=255)                                                        cGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTATAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACCGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATc                                                                                                                                                                                     >  2:550443/1‑251 (MQ=255)                                                          gTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAAAGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGTCGATGGTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTGATACAGTCCAAAACCTTTCTCGGTCAGGCTGGCGGACGGCAACATcac                                                                                                                                                                                   >  2:23527/1‑249 (MQ=255)                                                                acggcggcaccgataagggCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATAATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGCTGTTTTTGCCTGCGATA‑TCTGGCGCCAAGCCGCCCGCCCGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGACGGCCACATCCCCATCg                                                                                                                                                                              >  2:338934/17‑250 (MQ=255)                                                                    cggcAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGcc                                                                                                                                                                          >  1:450142/1‑250 (MQ=255)                                                                      gcAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGt                                                                                                                                                                       >  1:149332/1‑251 (MQ=255)                                                                             cccGGGCAAAATCCCCGGTGCGAATGCCTTCTTCTAAGGCGCGGTTAAGGGCGCGTTCAAGGG‑CGAAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGGG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCAtt                                                                                                                                                                <  1:81076/249‑1 (MQ=255)                                                                                gggCTAAATCCCCGGTGCGAATGCCTTTTTCTAATGCGCGGTTATTGGCGCGTTCAAGGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTgcg                                                                                                                                                             <  1:481207/251‑1 (MQ=255)                                                                                  gcaaaaccccgggggcgaagcccTTTTTCTAAGGGGCGGTAAATGGCCGGTTAAAGGG‑CGAAAGCCGCATCATCGGCACCCAGGCTGTAACGCAGCAGCAGGGCAAGCGAAAGGTTTTGGG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCAAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCa                                                                                                                                                           <  2:180190/230‑1 (MQ=255)                                                                                       cccccgggggggagtcccTTCTGCTAGTGCGGGGTTAGGGGCGCGTACAAGGG‑CGAACGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCAAGCGAAAGGTTTTGGG‑CAAGCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGt                                                                                                                                                      <  2:180221/235‑1 (MQ=255)                                                                                          cacGGTGCGAAGGCCTTTTTATATTGCGCGGTTAATGGCGCGTTAAATGG‑CGAAAGCCGAATCATCGGAATCCAGGTTTTAACGCAGAAGCAGTGCCAGCGAAAGGATTTGTG‑AAATCGGGTTGGCAATGTTTTTGCATGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTcag                                                                                                                                                   <  1:322670/249‑1 (MQ=255)                                                                                            cGGTGAGAATGCCTTCTTCTAATGCGCGGTTAGGGGCGCTTTCAATGG‑CGCAAGCCGAATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTcagac                                                                                                                                                 <  1:366628/251‑1 (MQ=255)                                                                                                 cGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAAt                                                                                                                                            >  1:342379/1‑251 (MQ=255)                                                                                                     tGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTTTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGACGGAAGGCAAAATCCCCATCGACCCAGGGATCATTGCGCACTCGTCAGACAGAATGTCg                                                                                                                                        >  1:11767/1‑251 (MQ=255)                                                                                                         ttttcatattGCGGGGTTAATGGCGCTTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTACCGCAGCAGCAGTGCCAGCGAAAGGTTTTGTG‑CAATCGGGTGGGCGATTTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGcc                                                                                                                                      <  1:174644/240‑1 (MQ=255)                                                                                                               tAATGAGCGGTTAATGGCGCGTTCAATGG‑AGCACGCCGCATCATCGGCATCCAAGCTGTAACGCAGCAGCAGTGCAAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGCTGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATAAAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACCTCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAggg                                                                                                                              >  2:236024/1‑250 (MQ=255)                                                                                                                                 gcggacaaggg‑CGCAGGCCGCCTCATCGGCTTCCAGGCTTTAACGCAGCAGCAG‑GTCAGCAAAAGGATTTGGG‑CAATCGGGTTGGCTATGTTTTTGCCTGCGATA‑TCGGGGCCCAAGCCCCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGTCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCGCTCGGTAGGCAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTc                                                                                                           <  1:66932/242‑1 (MQ=255)                                                                                                                                     taaaTGG‑CCCAAGCCGCATAATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCaa                                                                                                         <  1:89333/248‑1 (MQ=255)                                                                                                                                       aaTGG‑CGCAAGCCGCATAATCGGCATCCAGGCCGTAACGCAGCAGCAGTGCAAGCGAAAGGATTTGTG‑CAATCGGGTCGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACATTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATGGCGCACTCGGCAGACAGAATGTCGCCAACCAGGTTGGAGCCAAGCAGAACGTCAAACt                                                                                                      >  1:46802/1‑251 (MQ=255)                                                                                                                                        aggg‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGTATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACtg                                                                                                     <  1:521816/249‑1 (MQ=255)                                                                                                                                                acgccccatcatCGGCATCCAGGCTGTACCGCGGCAGCAGTGCCAGCGAAAGGATT‑GTGGCAATCGGGTTGGCGTTTTTTTGGCCTGCGATA‑TCTGGTCCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGa                                                                                              <  2:376605/245‑1 (MQ=255)                                                                                                                                                   ccGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATTTTTTTGCCTGCGATA‑TCGGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCt                                                                                           <  1:11526/251‑1 (MQ=255)                                                                                                                                                    cgctacagcGGCACCCAGGCTGTAACGCGGCAGCAGGGCGGGCGAAGGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATT‑GCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCtt                                                                                          <  2:219381/243‑1 (MQ=255)                                                                                                                                                     gcatcgccgccagccAGGCGGTACCGCAGCGGAAGTGCCAGGAAAAGTTTTTTTG‑CAATGGGGTGGACGATGTTTTTGCCTGC‑ATAATCGGGGGCAAAGCCCCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCAGAGCATAACGTCAAACGGGGAGGGATCttt                                                                                         <  2:569076/238‑1 (MQ=255)                                                                                                                                                     ccatcatCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTGGTG‑CACGCGGGTGGGCGATTTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCttt                                                                                         <  2:79068/250‑1 (MQ=255)                                                                                                                                                        ttaccgcgttccggcctttaccgttgcagccgTGCCAGGGAAAGTATTTGTT‑CAATCGGGTTGGAGATTTTTTTGTTGGCGATA‑TCTGGTCCCAGGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTGCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCTTCAGACAGAATGTCGCCAAACAGGTTGGAGCACATCAGAACGTCAAACTGTGATGTATCTTTaa                                                                                       <  1:184494/225‑1 (MQ=255)                                                                                                                                                           tcGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAAAGTCAAACTGTGATGGATCTTTAATc                                                                                     >  2:572816/1‑249 (MQ=255)                                                                                                                                                                   cAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTAGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAACCTGTGATGGATCTTTAATCAGCTGCAtgg                                                                           >  2:299436/1‑251 (MQ=255)                                                                                                                                                                           aaCGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTg                                                                   >  2:212867/1‑251 (MQ=255)                                                                                                                                                                                  cagcagTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCAAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt                                                            <  1:426416/251‑1 (MQ=255)                                                                                                                                                                                  cagcagTGCCAGCGAAAGGATCTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt                                                            >  2:357935/1‑251 (MQ=255)                                                                                                                                                                                   agcagTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGACGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt                                                            >  2:489273/1‑250 (MQ=255)                                                                                                                                                                                      agTGCCAGAAAAAGGATTGGTG‑AAATGGGGTGGGGGATGTTTTTGCCTGCGATA‑TCGGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACat                                                        <  2:374386/251‑1 (MQ=255)                                                                                                                                                                                        ggCCAGCGAAAGATTTTGTG‑AAATGGGGTGGGCGTTGTTTTTGCCGGAGATA‑TCGGGGGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACatat                                                      <  2:418347/250‑1 (MQ=255)                                                                                                                                                                                           aaGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGTCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGCGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATTTgcg                                                   >  2:21641/2‑251 (MQ=255)                                                                                                                                                                                                    gATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGAACATATGCGCCAGTTCg                                           >  2:542347/1‑250 (MQ=255)                                                                                                                                                                                                         gtg‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATcc                                     <  1:446008/251‑1 (MQ=255)                                                                                                                                                                                                           g‑CAATCGGGTTGGCGATTTTTTTGCCTGCGATA‑TCTGGGGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCgg                                   <  1:7826/251‑1 (MQ=255)                                                                                                                                                                                                             cAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCggg                                  >  1:1320/1‑251 (MQ=255)                                                                                                                                                                                                              aaTCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAACCTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGt                                 >  1:93809/1‑251 (MQ=255)                                                                                                                                                                                                                     ttGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGt                          >  2:339948/1‑251 (MQ=255)                                                                                                                                                                                                                             gTTTTTGCCTGCGATA‑TCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGAtct                  <  1:15676/251‑1 (MQ=255)                                                                                                                                                                                                                                              tCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCACCATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCg  >  2:281380/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  AGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGG‑CGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTG‑CAATCGGGTTGGCGATGTTTTTGCCTGCGATA‑TCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCG  >  NC_000913/80859‑81343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑