Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	658,024	G→T	intergenic (‑86/‑923)	crcB ← / → tatE	F(‑) channel protein/twin arginine protein translocation system ‑ TatE protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	658,024	0	G	T	100.0%	207.7 / NA	57	intergenic (‑86/‑923)	crcB/tatE	F(‑) channel protein/twin arginine protein translocation system ‑ TatE protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (35/22);  total (35/22)

GAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAA‑TCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGAGGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCGAGAAAGTAAACGTAACATGATGACGACAA  >  NC_000913/657783‑658267                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     gAATCCTATTCCTATGATGAATGCCCCAATCAGGCTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAA‑TCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATACGTGTAACGCCGAATAATCGTCGTTGGTGAATTTTACGACTCTGACAGGCTGTGGCattg                                                                                                                                                                                                                                             >  2:119218/1‑248 (MQ=255)          tcctatGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAA‑TCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGcc                                                                                                                                                                                                                                    >  1:126562/1‑251 (MQ=255)                gatgaATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAA‑TCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAgg                                                                                                                                                                                                                               >  2:101974/1‑250 (MQ=255)                 atgaATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAA‑TCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGAc                                                                                                                                                                                                                             >  1:539685/1‑251 (MQ=255)                  ggaATCCCCCAATCAGGTTTGCTGTCAGCGCCCCCACCGAAATCGCCTGATGCAGGGGTTTAAA‑TCGCATACTTAACAGCCACCTCGCCCCGCTTCCCGTCCCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACa                                                                                                                                                                                                                            <  1:101974/250‑1 (MQ=255)                          ccAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAA‑TCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTg                                                                                                                                                                                                                    >  1:422139/1‑251 (MQ=255)                            aataagttttGCTCTCAGCGTCCCCAACGGAACCGCCTGCTGCAGTGG‑TTAAACTCGCATACTTAACAGCCATCTCGCCACGCTCCCCGTCCCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGtt                                                                                                                                                                                                                  <  2:40004/244‑1 (MQ=255)                                 ggTTTGCTGTCGGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTAAAA‑TCCCATACTAAACAGCCATCTCGCCACGCTTCCCTTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATc                                                                                                                                                                                                             <  1:352738/251‑1 (MQ=255)                                      gCTGTCAGCCCCCCCAACGAAATCGCCTGATGCAGTGGGTTAAA‑TCGCATACTTAACAGCCATCTCGCCACGTGCCCCGTCCCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCtgtgt                                                                                                                                                                                                        <  1:232254/251‑1 (MQ=255)                                       cTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAA‑TCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCtgtgtg                                                                                                                                                                                                       >  2:171307/1‑251 (MQ=255)                                       cTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAA‑TCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCtgtgtg                                                                                                                                                                                                       >  2:168627/1‑251 (MQ=255)                                         gTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGTTTAAA‑TCGCATACTTAACAGCCATCCCCCCACGCTCCCCGTCCCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCtgtgtg                                                                                                                                                                                                       <  1:170825/249‑1 (MQ=255)                                                          aaTCGCCTGATGCAGTGGGTTAAA‑TCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGAtt                                                                                                                                                                                    >  1:44859/1‑251 (MQ=255)                                                              cctggttGCAGTGGGTTAAA‑TCGCATACTTAACAGCCCTCTCTCCACTCTTCCCTTCCCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTtgtg                                                                                                                                                                                <  1:498920/245‑1 (MQ=255)                                                               ccTGATGCAGTGGGTTAAA‑TCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGATGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGc                                                                                                                                                                               >  2:160966/1‑251 (MQ=255)                                                                   aTGCAGTGGGTTAAA‑TCGCATCCTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑g                                                                                                                                                                          >  1:23147/1‑251 (MQ=255)                                                                          gggTTAAA‑TCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGACTTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATgg                                                                                                                                                                   >  2:268803/1‑251 (MQ=255)                                                                           ggTTAAA‑TCGCATACTTCACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATACTCGTCGTTGGCGAATTTTACGACTCTGCCAGTATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAACGAACGCTGAGATTTGTGCCTC‑GTTGATGgc                                                                                                                                                                  >  2:426202/1‑251 (MQ=255)                                                                                    cGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAggcgg                                                                                                                                                          >  1:90836/1‑251 (MQ=255)                                                                                            tAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAac                                                                                                                                                  >  1:22878/1‑251 (MQ=255)                                                                                            aaaaccccATCTCCCCCCGCTCCCCTTACAACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGaaaa                                                                                                                                                   <  2:422139/244‑1 (MQ=255)                                                                                               cAGCCATCTCGCCACGCTTCCCGTACCACCGCCACTAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTCTTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAacgac                                                                                                                                               >  2:94676/1‑251 (MQ=255)                                                                                                       cctcccccctacccGTGCCGCCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTGTGAGTGATAAGTGTAGCGGCGGATAAGCGTCGTTGGCGAATTTTACGACTCTGATAGGATGTTGCAATGCTGGTTGCCGCAGGACAGATTGCTGTTACGTCGGTGTGGGAAAAGAACACTGAGATTTGTATCTC‑CTTGATGGGGCAGTCGGCGCATAACGACGCATCGCt                                                                                                                                       <  2:355751/240‑1 (MQ=255)                                                                                                         gCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACCCTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGt                                                                                                                                     >  1:360847/1‑251 (MQ=255)                                                                                                         gCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGt                                                                                                                                     >  2:40051/1‑251 (MQ=255)                                                                                                            cctcTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTg                                                                                                                                  <  2:47711/248‑1 (MQ=255)                                                                                                                            cGCCAATAAAAACTGCTAAAAGAAGTTGTCACACTGCCAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCAtt                                                                                                                  >  2:545578/1‑251 (MQ=255)                                                                                                                               cAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCt                                                                                                               >  1:68957/1‑251 (MQ=255)                                                                                                                                     aaaCTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGgcgcg                                                                                                         <  2:569734/251‑1 (MQ=255)                                                                                                                                     aaaCTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGCTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGgcgcg                                                                                                         >  2:391903/1‑251 (MQ=255)                                                                                                                                       aCTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGcgcgcg                                                                                                       <  1:433848/251‑1 (MQ=255)                                                                                                                                             aaaaGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCTCAGGACAGTTTGCTGTTAGATCTGTGTTGGAAAAGAACGCTGAGATTTGTGCCTG‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCGTGGCCGGTAGCATTGCTGGTGCGCgatga                                                                                                   <  1:196094/249‑1 (MQ=255)                                                                                                                                               aagaagTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGAGGATCat                                                                                               <  2:92457/251‑1 (MQ=255)                                                                                                                                                agaagTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCatg                                                                                              <  1:90187/251‑1 (MQ=255)                                                                                                                                                   agTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCatgatg                                                                                           >  1:475302/1‑251 (MQ=255)                                                                                                                                                    gTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGc                                                                                          >  1:382376/1‑251 (MQ=255)                                                                                                                                                          acacTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCAGGATGCAGATCt                                                                                    <  2:326843/251‑1 (MQ=255)                                                                                                                                                           cacTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGCTGCAGATCtt                                                                                   >  2:28957/1‑250 (MQ=255)                                                                                                                                                            acTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGACTTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTAt                                                                                  >  2:91514/1‑251 (MQ=255)                                                                                                                                                                caaattcgtGCTATTTGATTTGTATGAGTGATCAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCCATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGCAAACGAAGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGAGATGCTCATGATGCAGATCTATCGGt                                                                              >  2:42034/9‑251 (MQ=255)                                                                                                                                                                    aTCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTaa                                                                           >  1:270763/1‑250 (MQ=255)                                                                                                                                                                      ccTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAAt                                                                         <  2:450993/250‑1 (MQ=255)                                                                                                                                                                       cTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCATGGTTGGTGGCGGCAGGACAGTTTGTTGTTACTCCTGTGTGGGAAAGGAACGCTGA‑ATTTGGGGCTCCGTTGAGGGGGAAGGCGGCGGAAAACGACGATTCGTTGTTTGCCTTGCCGGAAGATTTGTTGGCGCGGGATGATCTTGATCCAGCGCTATCGGTTaatta                                                                        >  1:464417/1‑247 (MQ=255)                                                                                                                                                                         gCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGc                                                                     <  1:323009/251‑1 (MQ=255)                                                                                                                                                                            atttgatttgTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAATGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGcac                                                                   >  2:400990/1‑250 (MQ=255)                                                                                                                                                                            atttgatttgTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGcac                                                                   >  2:76263/1‑250 (MQ=255)                                                                                                                                                                                gatttgTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTTCATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGATGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCGCAgctg                                                              <  2:249429/251‑1 (MQ=255)                                                                                                                                                                                gatttgTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAgctg                                                              <  1:382575/251‑1 (MQ=255)                                                                                                                                                                                         gAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGc                                                     <  1:40051/251‑1 (MQ=255)                                                                                                                                                                                          aGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCg                                                    >  1:244953/1‑251 (MQ=255)                                                                                                                                                                                          aGTGATAAGTGAAACGCCGAATAGTAGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCg                                                    >  2:568241/1‑251 (MQ=255)                                                                                                                                                                                                     tAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCgg                                          >  2:450002/1‑250 (MQ=255)                                                                                                                                                                                                          ccGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAACGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACttt                                     >  1:245205/1‑250 (MQ=255)                                                                                                                                                                                                                    gtcgtTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCGAGaaa                          <  2:195304/251‑1 (MQ=255)                                                                                                                                                                                                                      cgtTGGCGAATTTTACGACTCTGACAGGATGTGGCAATGTTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGCAAAGAACGCTGAGATTTGTGCCTC‑GTTGCTGGCGCAGGCGGCGGAAACCGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCGAGAAAGt                        >  1:324196/1‑251 (MQ=255)                                                                                                                                                                                                                                  ttACGACTCTGACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCGAGAAAGTAAACGTAACatg            >  2:21466/1‑251 (MQ=255)                                                                                                                                                                                                                                            gACAGGATGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTGGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCGGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTACCGGTTAA‑TCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCGAGAAAGTAAACGTAACATGATGACGACaa  <  1:231737/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     GAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAA‑TCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTACGACTCTGACAGGAGGTGGCAATGCTGGTTGCCGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTC‑GTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCGAGAAAGTAAACGTAACATGATGACGACAA  >  NC_000913/657783‑658267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑