Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,562,396	A→G	L94L (TTG→CTG)	yjiJ ←	putative transporter YjiJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,562,396	0	A	G	100.0%	221.4 / NA	60	L94L (TTG→CTG)	yjiJ	putative transporter YjiJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/32);  total (28/32)

CCTTGCCATAACGTTTGCGAGGAGAGGTCAAAATGCAGGCCTGCCAGAACATATTCATTGCCCAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCATACCTAAGGTCAGAACCAGCATCCCGAAAAGCGCGGTGCTGAA  >  NC_000913/4562155‑4562639                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    ccTTGCCATAACGTTTGCGAGGAGAGTTCAAAATGCAGGCCTGCCAGAACATATTCATTGCCCAGTGCGATGCCAATGCCACCGCCAGAAACCAAAGCTGCCACCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGCCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATTGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGc                                                                                                                                                                                                                                            <  1:264/251‑1 (MQ=255)     gCCATAACGTTGGCGAGGAGAGGTCAAAATGCAGGCCTGCAAGAACATATTCATTGCCCAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGAGGGCGCGTGTGCTGCATAAGCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAg                                                                                                                                                                                                                                         <  1:334344/250‑1 (MQ=255)       cATAACGTTTGCGAGGAGAGGTCAAAATGCAGGCCTGCCAGAACATATTCATTGCCCAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGc                                                                                                                                                                                                                                      >  1:500180/1‑251 (MQ=255)          aaCGTTTGCGAGGAGAGGTCAAAATGCAGGCCTGCCAGAACATATTCATTGCCCAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAgcgc                                                                                                                                                                                                                                      >  2:527369/1‑245 (MQ=255)              tttGCGAGGAGAGGTCAAAATGCAGGCCTGCCAGAACATATTCATTGCCCAGTGCGATGCCAACGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGcc                                                                                                                                                                                                                               >  1:294761/1‑251 (MQ=255)               ttGCGAGGAGAGGTCAAAATGCAGGCCTGCCAGAACATATTCATTGCCCAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCGGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGcc                                                                                                                                                                                                                               <  1:256286/250‑1 (MQ=255)                 gCGAGGAGAGGTCAAAATGCAGGCCTGCCAGAACATATTCATTGCCCACTGCGATGCCAATGCCAACGCAAGAAAACAAAGCTGCCAGCACAAAAGGATGCCGCGTGTGCTGCCTAATCAGCGTCGAACCAAAAATCAGCATACAGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCCGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAAc                                                                                                                                                                                                                            >  1:33582/1‑251 (MQ=255)                                   gCAGGCCTGCCAGAACATATTCATTGCCCAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGgcg                                                                                                                                                                                                           <  1:471974/250‑1 (MQ=255)                                       gCCTGCCAGAACATATTCATTGCCCAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATg                                                                                                                                                                                                      >  1:376960/1‑251 (MQ=255)                                            ccAGAACATATTCATTGCCCAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTgg                                                                                                                                                                                                 >  2:116312/1‑251 (MQ=255)                                            ccAGAACATATTCATTGCCCAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTgg                                                                                                                                                                                                 <  1:318709/251‑1 (MQ=255)                                             cAGAACAAAATCATTGCCCAGTGAGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGAGGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGt                                                                                                                                                                                                <  2:67559/251‑1 (MQ=255)                                                  aatatTCATTGCCCAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAAt                                                                                                                                                                                           <  1:188978/250‑1 (MQ=255)                                                     atTCATTGCCAAGTGCGATCCCAATTCCAACGCCAGAAAACAAACCTGCCAGCACAAAAGGAGGGCGCGTGTGCGGCATAACCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGTCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATgc                                                                                                                                                                                         <  2:277889/250‑1 (MQ=255)                                                           ggCCCAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCGGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACAAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGAGCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCaaaa                                                                                                                                                                                  <  2:489166/250‑1 (MQ=255)                                                             cccAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCaaa                                                                                                                                                                                   >  1:177083/1‑248 (MQ=255)                                                                     gaggccaatgccaaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCAAACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCa                                                                                                                                                                        <  2:369758/248‑1 (MQ=255)                                                                        gccaatgccacCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAACCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGa                                                                                                                                                                      <  2:135626/250‑1 (MQ=255)                                                                          caatgccaaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTg                                                                                                                                                                   >  1:225455/1‑251 (MQ=255)                                                                                 aaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTgccagcca                                                                                                                                                            <  1:345387/251‑1 (MQ=255)                                                                                 aaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTgccagcc                                                                                                                                                             >  1:42694/1‑250 (MQ=255)                                                                                  ccGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGccagccag                                                                                                                                                           <  2:107658/250‑1 (MQ=255)                                                                                      cAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATAc                                                                                                                                                       >  1:251921/1‑251 (MQ=255)                                                                                        gAAAACACAGCTGCCAGCACAAAAGGATGGAGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCCGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCCACAGCCCGCTCGCCAGGGCAGAAGCCAACCGCAATGCGCGCCGGCGCGCTGGCTGGTGCAATGCGCCAAAAGAAACTAGCAGACTGCCCGCCAGATAccc                                                                                                                                                     >  2:345206/1‑251 (MQ=255)                                                                                          aaaCAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCg                                                                                                                                                   >  2:257892/1‑251 (MQ=255)                                                                                                    gCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGcc                                                                                                                                         >  1:218428/1‑251 (MQ=255)                                                                                                      cAGCACAAAAGGATGGCGCGTTTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAAGCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAATAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGc                                                                                                                                       <  1:261969/251‑1 (MQ=255)                                                                                                           caAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCa                                                                                                                                  <  1:302854/251‑1 (MQ=255)                                                                                                               aggaggGCGCGGGTGCGGCATAATCAGCGTCGAACAAAAAATAAGCATCCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATcc                                                                                                                              <  2:392758/251‑1 (MQ=255)                                                                                                                          tgtgCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGcc                                                                                                                                         <  2:546520/229‑1 (MQ=255)                                                                                                                          tgtgCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGcc                                                                                                                                         >  1:546520/1‑229 (MQ=255)                                                                                                                            tgctgcATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCt                                                                                                                 >  1:86579/1‑251 (MQ=255)                                                                                                                                gcATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGAAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTCAAt                                                                                                             >  1:445001/1‑251 (MQ=255)                                                                                                                                     agcagcttagaccaaaaaaaCAGAATACCGGCGCGGGCGACACCCGGCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTGGCCGCTGGCAATCCACGAGAGCTGGCTAAATGaaaa                                                                                                        <  2:306247/237‑1 (MQ=255)                                                                                                                                       cAGCGTCGAACCAAAAATCGGCATCCCGGCGCTGGCGCCCCCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACg                                                                                                      <  2:500180/251‑1 (MQ=255)                                                                                                                                          cGTCGAACCAAAAAGCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATc                                                                                                   <  1:305987/251‑1 (MQ=255)                                                                                                                                           gTCGAACAAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATcc                                                                                                  <  1:469799/251‑1 (MQ=255)                                                                                                                                             cGAACAAAAAATCAGCATCCCGGCGCTGGCGCCCCCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAAACCACGAGAGCTGGCTAAATGAAAACGATCCtt                                                                                                <  2:47514/251‑1 (MQ=255)                                                                                                                                                       aTCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCcatcat                                                                                      <  1:93984/251‑1 (MQ=255)                                                                                                                                                           gCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACg                                                                                  <  1:147206/251‑1 (MQ=255)                                                                                                                                                             aTACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACggg                                                                                >  1:382174/1‑251 (MQ=255)                                                                                                                                                                  ggCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAAc                                                                            >  1:444463/1‑250 (MQ=255)                                                                                                                                                                        ggCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCg                                                                     >  1:355170/1‑251 (MQ=255)                                                                                                                                                                                   ccAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGa                                                            >  1:453859/1‑249 (MQ=255)                                                                                                                                                                                    cAGGAAGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGc                                                         <  2:445412/251‑1 (MQ=255)                                                                                                                                                                                     aGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCg                                                        >  1:37864/1‑251 (MQ=255)                                                                                                                                                                                       gACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTGCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGc                                                      <  1:68720/251‑1 (MQ=255)                                                                                                                                                                                                         cAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCATACCTAAgg                                    <  1:483710/251‑1 (MQ=255)                                                                                                                                                                                                           gAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCATACCTAAGGTc                                  >  1:242724/1‑251 (MQ=255)                                                                                                                                                                                                           gAATAAAGGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCATACCTAAGGTc                                  <  2:274029/251‑1 (MQ=255)                                                                                                                                                                                                            aaTAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTgccagcc                                                                                                                                                             >  1:251510/1‑127 (MQ=255)                                                                                                                                                                                                            aaTAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTgccagcc                                                                                                                                                             <  2:251510/127‑1 (MQ=255)                                                                                                                                                                                                               aaaTGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCATACCTAAGGTCAGa                               >  2:556501/1‑250 (MQ=255)                                                                                                                                                                                                                   ggcggcAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCATACCTAAGGTCAGAACCa                           <  1:455938/250‑1 (MQ=255)                                                                                                                                                                                                                   ggcggcAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGAGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCATACCTAAGGTCAGAAcc                            >  1:281363/1‑249 (MQ=255)                                                                                                                                                                                                                          aCCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGCAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCATACCTAAGGTCAGAACCAGCATCCCg                   >  2:335870/1‑251 (MQ=255)                                                                                                                                                                                                                             aTGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCATACCTAAGGTCAGAACCAGCATCCCGaa                 >  1:280171/1‑250 (MQ=255)                                                                                                                                                                                                                                     gCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCATACCTAAGGTCAGAACCAGCATCCCGAAAAGCGCgg         <  2:13913/250‑1 (MQ=255)                                                                                                                                                                                                                                        aGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCATACCTAAGGTCAGAACCAGCATCCCGAAAAGCGCGGTGCt     <  2:107151/251‑1 (MQ=255)                                                                                                                                                                                                                                            tCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCATACCTAAGGTCAGAACCAGCATCCCGAAAAGCGCGGTGCTgaa  >  2:142367/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    CCTTGCCATAACGTTTGCGAGGAGAGGTCAAAATGCAGGCCTGCCAGAACATATTCATTGCCCAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCATACCTAAGGTCAGAACCAGCATCCCGAAAAGCGCGGTGCTGAA  >  NC_000913/4562155‑4562639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑