Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,489,016	C→T	E350K (GAA→AAA)	yjgR ←	DUF853 domain‑containing protein YjgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,489,016	0	C	T	100.0%	242.5 / NA	65	E350K (GAA→AAA)	yjgR	DUF853 domain‑containing protein YjgR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/38);  total (27/38)

GGTACTGGCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTCGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATG  >  NC_000913/4488777‑4489259                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    ggTACTGGCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCGTTTTGGTATCAAACg                                                                                                                                                                                                                                          <  1:224409/251‑1 (MQ=255)  gTACTGGCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGc                                                                                                                                                                                                                                         >  1:355209/1‑251 (MQ=255)  gTACTGGCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGc                                                                                                                                                                                                                                         <  2:423554/251‑1 (MQ=255)    aCTGGCCTGAAAGCTTTTTTGCAACATCGCATAGGCGGATTCCCGGTCCCCCTCATCCTCATATTTGCCATACACCGGTGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGcc                                                                                                                                                                                                                                        <  2:116741/250‑1 (MQ=255)       ggCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGAt                                                                                                                                                                                                                                    <  1:295888/251‑1 (MQ=255)             aaaGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGccc                                                                                                                                                                                                                              >  1:174202/1‑251 (MQ=255)                 ccTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCAt                                                                                                                                                                                                                          >  2:93530/1‑251 (MQ=255)                           ccatctaatcggctgcttCCCGGTCCTCCTCATCCTCATATTTCCCATACACCGGAGA‑TGTATAAATCAAGCCATTACGCTCATCTTCCGTCCCCGGCCCCATCCGCTAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGTAAAGAGATCAACTCCTCGCCGGTGTCCAGTGCCTGAATCGCCGTTTTGGTATCAAACGCCTGATTGGCCCGCATGGTTTGcgcc                                                                                                                                                                                                                <  2:330076/235‑1 (MQ=255)                                 cATAGGCGGATTCCCGGTCCACCACATCCTCATATTTGCCATACACCGGAGAGTG‑ATTAATCAAGCCATTCCGCTCATCTTCCGTCCCCGGCCCCATCCGCGAACAAGTCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCTCCGCt                                                                                                                                                                                                             <  2:328273/248‑1 (MQ=255)                                      gCGGATTCCCGGCCCACCTCATCCTCATATTTGCCATACACCGGAGAGGG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCGCCGGCCCCAGCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAGCGCCGGATTGGCCCGCATGGTTTGCGCCTCTGCCTTCAc                                                                                                                                                                                                     <  2:85258/251‑1 (MQ=255)                                         gATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGc                                                                                                                                                                                                  <  2:196350/251‑1 (MQ=255)                                             cccGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCttt                                                                                                                                                                                               <  1:93530/250‑1 (MQ=255)                                                         catcctcatATTTGCCATACCCCGGAGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAAGCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTGTGCGCCGCTGCCTTCACTGCCTTCGGATCTTTTGGc                                                                                                                                                                                  <  2:293445/251‑1 (MQ=255)                                                         catcctcatATTTGCCATACACCGGAGAGTG‑ATTACTCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGc                                                                                                                                                                                  >  2:378461/1‑251 (MQ=255)                                                               catATTTGCCATACACCGGAGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTaaaa                                                                                                                                                                            <  1:511732/251‑1 (MQ=255)                                                               catATTTGCCATACACCGGAGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTaaa                                                                                                                                                                             >  2:206469/1‑250 (MQ=255)                                                               catATTTGCCATACACCGGAGAGTG‑ATTAATCAAGCCATTACGCTCATCGTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTaa                                                                                                                                                                              <  2:149185/249‑1 (MQ=255)                                                                atatTTGCCATCCCCCGGATACTT‑ATTAATCACGCCATTACGCTCATCTTCCTTCACCGGCCCCATCCTCGAACAATGCTCGATCACCATCGCCCGCTCCCCCACAGAAGGGCTTCCTTTCGCAGCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATGGGCCCGCAGGGTTTGAGCCGCTGCCGTCACTGCTTTCGGAGCTTTTGTCGTAAAAg                                                                                                                                                                           <  2:508441/251‑1 (MQ=255)                                                                                ggAGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACAGGCCCCATCCGCGAACACGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTg                                                                                                                                                           >  1:328458/1‑251 (MQ=255)                                                                                  agcatG‑ATTAACCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGaa                                                                                                                                                         <  2:60573/247‑1 (MQ=255)                                                                                   gagTG‑ATTAATCAAGCCATTACGCTCATCGTCCATCACCGGCCCCATCCGCGAACAAGGCGCGAGCACCATCGCACGCTCCACCACAGAAGGGCTTCCTGTCTCATCCAGAAAAGAGATCAACGCCTCGCCGTTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATGGGCCCGCATGGTTGGCGCCGCTGCCTGCACTGCGTTCGGATCTTTTGGCGTAACAGCTCGCTAATAATGTTGAAc                                                                                                                                                        <  2:272897/251‑1 (MQ=255)                                                                                      tG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAAcgcg                                                                                                                                                     >  1:296570/1‑251 (MQ=255)                                                                                      tG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAAcgcg                                                                                                                                                     >  1:208251/1‑251 (MQ=255)                                                                                       g‑ATTAATCAAGCCATTACGCTCATCTTCCGTCCCCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGa                                                                                                                                                    <  1:74499/251‑1 (MQ=255)                                                                                         aTTAATCAAGCCATTACGCGCAGCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTGTTGGCGTAAAAGCCTGCAAAGCAGGTTGAACGCGAt                                                                                                                                                   <  2:24414/251‑1 (MQ=255)                                                                                          ttAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACAATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGCGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCAGATCTTTTGGCGTAAAAGCCCGCAACGCATGTTGAACGCGAtt                                                                                                                                                  >  2:255968/1‑251 (MQ=255)                                                                                           tAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGTCGGTGCCCAGTTCCTGAAGCGCGTTTGTGGTATCAAACGCCGGATGGGCCCGCATGGTTTGCGCCGCTGCGTTCACTGCTTTCTGATGTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACTCGAtt                                                                                                                                                  <  1:40559/250‑1 (MQ=255)                                                                                            aaTCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTAc                                                                                                                                                <  1:217922/251‑1 (MQ=255)                                                                                                aaGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCg                                                                                                                                              >  2:209955/1‑249 (MQ=255)                                                                                                    cATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGc                                                                                                                                        >  2:458326/1‑251 (MQ=255)                                                                                                     aTTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGcc                                                                                                                                       >  1:8308/1‑251 (MQ=255)                                                                                                       tACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGccc                                                                                                                                      <  2:362306/250‑1 (MQ=255)                                                                                                                  tCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTAt                                                                                                                           <  1:139635/250‑1 (MQ=255)                                                                                                                   ccGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATc                                                                                                                          <  2:6934/250‑1 (MQ=255)                                                                                                                       cACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGa                                                                                                                      >  2:151780/1‑250 (MQ=255)                                                                                                                            gCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAACAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATc                                                                                                                 >  1:344145/1‑250 (MQ=255)                                                                                                                            gCCCCATCCGCGAACAAGGCGAGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTCAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATc                                                                                                                 >  2:485708/1‑250 (MQ=255)                                                                                                                                   ccGCGAACGAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTGTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACGGCTTTCTGATCTTTGGTCGTAAAAGCCCGCAAAGCATGTTGAGCGCGATTACGGAGCTGCCCGAGCACATGATCCGGAATATCAGACggg                                                                                                          <  2:74492/250‑1 (MQ=255)                                                                                                                                      cgAACAAGGCGCGATCGCCACCGCACGCTCCACCACAGAATGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCGCGCCGGTGCCCAGTTCCTGAATCGCCGTTTTGGTATCAAACTCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCGTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGATCACATGATCCGGAATAGCAGACGGGtttt                                                                                                      <  2:13298/251‑1 (MQ=255)                                                                                                                                       gAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTg                                                                                                     <  1:186103/251‑1 (MQ=255)                                                                                                                                                           cGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCa                                                                                 <  1:557097/251‑1 (MQ=255)                                                                                                                                                               cGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGcc                                                                             >  1:237461/1‑251 (MQ=255)                                                                                                                                                                gCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCt                                                                            <  1:155689/251‑1 (MQ=255)                                                                                                                                                                   ccaccaCAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCttt                                                                          <  1:458326/250‑1 (MQ=255)                                                                                                                                                                            aaGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAAt                                                                 >  2:565299/1‑250 (MQ=255)                                                                                                                                                                              gggCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGc                                                                     <  2:311080/244‑1 (MQ=255)                                                                                                                                                                              gggCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGc                                                                     >  1:311080/1‑244 (MQ=255)                                                                                                                                                                              gggCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATa                                                                >  2:137670/1‑249 (MQ=255)                                                                                                                                                                               ggCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGTTCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGc                                                             >  2:395439/1‑251 (MQ=255)                                                                                                                                                                               ggCTTCCGTTCGCATCCAGAAAAGAGATCAGCGCCTCGCCGGTGCCCATTTCCTGAATCTGCTGTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGGGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGAGTACCGAGCTGCCCGTGCACATTATCCGGAATATCAGGCGGGTTTTGCGAAACGAACCAGTCGCCCACGCCTTTTGAGCGAATAAGc                                                             <  2:20562/251‑1 (MQ=255)                                                                                                                                                                                    ccTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTAt                                                        <  2:13877/251‑1 (MQ=255)                                                                                                                                                                                        tCGCATCCAGAAAAGCGATCACCGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGCTTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAACAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCAcc                                                    >  2:564972/1‑251 (MQ=255)                                                                                                                                                                                         cGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCAcc                                                    <  2:175683/250‑1 (MQ=255)                                                                                                                                                                                         cGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCt                                                   <  2:103338/251‑1 (MQ=255)                                                                                                                                                                                                    aaGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTa                                        <  2:244752/251‑1 (MQ=255)                                                                                                                                                                                                         atcaacgaatcgacgcagCCCAGTTCCTGAACAGCATTTTTGGTATCAAACGCAGCATTGGCCCGCATGGTGTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGCGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAACCGACCCAGACGCCCCCGCCTTTGGAGCGAATACGACTTATCACCTGCTCACTCTTATcccc                                   >  2:286633/18‑249 (MQ=255)                                                                                                                                                                                                              cGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGt                               >  1:184632/1‑250 (MQ=255)                                                                                                                                                                                                                            ccAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCCTGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGTGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCTGGGCATCg                >  2:115992/1‑251 (MQ=255)                                                                                                                                                                                                                              aGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGtt              <  2:208251/251‑1 (MQ=255)                                                                                                                                                                                                                               gTTCCTGCATCGCCTCTTTGGTATCAACCGCCGGATTGGACCGCATGGCTTGCGCCGCTGCCTTCAATGCTTTCTGATCTTTTGGCGTAACAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGccgcgtt              >  2:363348/1‑244 (MQ=255)                                                                                                                                                                                                                                  ccTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGTCATCGTTAAAc          <  1:577807/251‑1 (MQ=255)                                                                                                                                                                                                                                  ccTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAAc          <  1:510243/251‑1 (MQ=255)                                                                                                                                                                                                                                     gAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAAcag        <  1:15824/250‑1 (MQ=255)                                                                                                                                                                                                                                        tCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAAcagc       >  2:442519/1‑248 (MQ=255)                                                                                                                                                                                                                                          gCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGAtg  >  1:516235/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    GGTACTGGCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTG‑ATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTCGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATG  >  NC_000913/4488777‑4489259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑