Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,368,641	G→T	intergenic (‑314/‑23)	aspA ← / → fxsA	aspartate ammonia‑lyase/protein FxsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,368,641	0	G	T	97.7%	160.8 / NA	44	intergenic (‑314/‑23)	aspA/fxsA	aspartate ammonia‑lyase/protein FxsA
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (20/23);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTACCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCGGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATT  >  NC_000913/4368407‑4368882                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  cgaTTATCCCCTGCATCGACCGAATACCCGAGATCATCTGCTGCTTGAGGATTTCTACCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATCGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAgg                                                                                                                                                                                                                                     >  1:45215/1‑249 (MQ=255)   aTTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTACCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGgaga                                                                                                                                                                                                                                  >  1:455629/1‑250 (MQ=255)           cTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTACCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTgc                                                                                                                                                                                                                         >  1:271015/1‑251 (MQ=255)             gCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTACCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTgc                                                                                                                                                                                                                         <  2:573147/249‑1 (MQ=255)                  gACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTACCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGtt                                                                                                                                                                                                                  <  2:348160/251‑1 (MQ=255)                      gAATACCCGAGATCATATGCTGCTTGAGGATTTCTACCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCt                                                                                                                                                                                                              >  1:40677/1‑251 (MQ=255)                                        gctgctTGAGGATTTCTACCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTTCCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTATCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCt                                                                                                                                                                                             <  2:278590/250‑1 (MQ=255)                                              tGAGGATTTCTACCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTAGTGCCATTTTCCGTTATg                                                                                                                                                                                        <  1:191678/249‑1 (MQ=255)                                              tGAGGATTTCTACCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCGGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATg                                                                                                                                                                                        <  2:354852/249‑1 (MQ=255)                                                    tttCTACCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTCTCCTTTATGTCTATAtt                                                                                                                                                                                >  2:201347/1‑251 (MQ=255)                                                     ttCTACCGTAATCTGGATCACGTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATAtt                                                                                                                                                                                <  1:191374/250‑1 (MQ=255)                                                      tCTACCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTg                                                                                                                                                                               <  2:486446/250‑1 (MQ=255)                                                         aCCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTgag                                                                                                                                                                             <  1:520547/249‑1 (MQ=255)                                                         aCCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATGTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGAt                                                                                                                                                                           <  2:382769/251‑1 (MQ=255)                                                                         cTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCACAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTc                                                                                                                                                             >  1:375661/1‑249 (MQ=255)                                                                                 gtCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGccc                                                                                                                                                    >  1:177548/1‑250 (MQ=255)                                                                                        tttACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTAtt                                                                                                                                             <  1:185999/250‑1 (MQ=255)                                                                                                      attaATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGAc                                                                                                                              >  2:109982/1‑251 (MQ=255)                                                                                                            ttGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGt                                                                                                                        >  1:420090/1‑251 (MQ=255)                                                                                                              gtgAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGc                                                                                                                      >  1:27145/1‑251 (MQ=255)                                                                                                                  aaTAGATCACCGCTTTGGGATTACTACCAAAAAGAGTTGCGCAAACAGCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTGCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCGTGGATTGGGGGTATTGCTGTCCCTCGTGCTGGt                                                                                                                  <  2:252335/251‑1 (MQ=255)                                                                                                                    tAGATCACCGCTTTGGGATTACTACCAAAACTAGATGCGCAAAAAACTTGAAATTTTGCTAATGACAACAATATACGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACACTCGTGCTGGtt                                                                                                                 >  1:525027/1‑250 (MQ=255)                                                                                                                    tAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACACTCGTGCTGGtt                                                                                                                 >  1:373626/1‑250 (MQ=255)                                                                                                                           ccGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCAc                                                                                                         >  2:538061/1‑251 (MQ=255)                                                                                                                                    gATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTAt                                                                                                <  2:371872/251‑1 (MQ=255)                                                                                                                                        actacCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTAAGTTATCGGt                                                                                            >  2:343643/1‑251 (MQ=255)                                                                                                                                              aaaaaTAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTct                                                                                      <  2:319335/251‑2 (MQ=255)                                                                                                                                                         cgcAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTa                                                                            >  1:442789/1‑250 (MQ=255)                                                                                                                                                             aaCATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAAcc                                                                         >  1:315676/1‑249 (MQ=255)                                                                                                                                                                          tttGCTAATGACCACAATATAAGCTAAACGCGATTCGCACCCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCTTTAAGAAtt                                                          >  1:288294/1‑251 (MQ=255)                                                                                                                                                                            tGCTACTGACCACACTATAAGCTAACCGCGATTCGCACCCCATTCAGGTCGCCGGGGTTAACCTGCTGCTATTACAGGAGAACCCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCACTCTTTATTCAGCTTGCCCATGTATTGGGGGTATTGCTGACCCt                                                                                                                           >  2:274459/1‑184 (MQ=255)                                                                                                                                                                            tGCTAATGACCACAATATAAGCTAAACGCTATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTGTCCTTTATGGCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCt                                                                                                                           <  1:274459/184‑1 (MQ=255)                                                                                                                                                                              cTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGt                                                      >  2:346024/1‑251 (MQ=255)                                                                                                                                                                                 aTGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCGTTAAGAAGTTCGTGCt                                                   <  1:503400/251‑1 (MQ=255)                                                                                                                                                                                     ccACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATg                                               <  2:307307/251‑1 (MQ=255)                                                                                                                                                                                            ataAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCaa                                         <  1:434671/250‑1 (MQ=255)                                                                                                                                                                                                   aaaCGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATggc                                 <  1:23055/251‑1 (MQ=255)                                                                                                                                                                                                          aTTCGCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAGAAAATGGCGGCGGGt                          <  1:458659/251‑1 (MQ=255)                                                                                                                                                                                                              gCAACCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGaaa                      <  2:185579/251‑1 (MQ=255)                                                                                                                                                                                                                aaCCCATTCAGGTAGCCGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAAc                    >  1:223503/1‑251 (MQ=255)                                                                                                                                                                                                                               ccGGGGTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAgatg     <  1:368264/251‑1 (MQ=255)                                                                                                                                                                                                                                 ggggTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAAGGGCGGCGGGTGAAAACCCAGCGGCGGAgatgat   <  2:53862/251‑1 (MQ=255)                                                                                                                                                                                                                                  gggTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGAtt  <  1:234661/251‑1 (MQ=255)                                                                                                                                                                                                                                  gggTTAACCTGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGAtt  <  2:455629/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  CGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTACCGTAATCTGGATCACTTTAAGTGTCGGTTTTTACCCCTTAATTATTAATTTGTGAAATAGATCACCGCTTTGGGATTACTACCAAAAATAGTTGCGCAAACATCTTGAAATTTTGCTAATGACCACAATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCGGCTGCTATTACAGGAGAAACCTTTGCGCTGGTTACCTTTTATTGCCATTTTCCTTTATGTCTATATTGAGATTTCAATCTTTATTCAGGTTGCCCATGTATTGGGGGTATTGCTGACCCTCGTGCTGGTTATATTCACGTCAGTTATCGGTATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATT  >  NC_000913/4368407‑4368882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑