Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,925,834	C→T	intergenic (‑201/+178)	mnmG ← / ← mioC	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG/flavoprotein MioC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,925,834	0	C	T	100.0%	156.9 / NA	43	intergenic (‑201/+178)	mnmG/mioC	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG/flavoprotein MioC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/25);  total (18/25)

CCCGCCAATGATGATGACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAACAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCA  >  NC_000913/3925592‑3926078                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     cccGCCAATGATGATGACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGGAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGa                                                                                                                                                                                                                                              <  2:462357/251‑1 (MQ=255) cccGCCAATGATGATGACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTgg                                                                                                                                                                                                                                               >  1:14016/1‑250 (MQ=255)  ccGCCAATGATGATGACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGaa                                                                                                                                                                                                                                             <  2:179352/251‑1 (MQ=255)     ccAATGATGATGACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAgg                                                                                                                                                                                                                                          >  2:172554/1‑251 (MQ=255)      cAATGATGATGACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGACGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATCACCTGGAAAgg                                                                                                                                                                                                                                          >  1:239015/1‑250 (MQ=255)        atgatgatgACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATc                                                                                                                                                                                                                                       <  1:305326/251‑1 (MQ=255)                        aaGGATCCGGATAAAACATTGTGATTGCCTCTCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGTGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGTAGCGCACTGCCCGTTTTATA‑ACAAGTATCCGGCTTTTAATATCAGTAATCTGGAAAGTAGCATTAACTTTGAATGAt                                                                                                                                                                                                                       <  2:572146/251‑1 (MQ=255)                           gATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCgg                                                                                                                                                                                                                    <  1:457419/251‑1 (MQ=255)                                              gATTTCCTCGCATAACTCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATaa                                                                                                                                                                                                  <  1:328746/250‑1 (MQ=255)                                               aTTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGc                                                                                                                                                                                                >  1:118536/1‑251 (MQ=255)                                                      cGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGa                                                                                                                                                                                           <  1:452733/249‑1 (MQ=255)                                                        cATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTATGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACGGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAg                                                                                                                                                                                       <  2:404213/251‑1 (MQ=255)                                                              gcgGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAg                                                                                                                                                                                 <  2:268429/251‑1 (MQ=255)                                                                   aTGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGt                                                                                                                                                                             <  1:170251/250‑1 (MQ=255)                                                                   aTGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGt                                                                                                                                                                             <  1:124199/250‑1 (MQ=255)                                                                     gAAAATGGATTTAACCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTTTGGATA‑ACGAGTATCCGGCTTTTAAGATGTATATCTCGGAAAGTATCAGTACCTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTtat                                                                                                                                                                          <  1:60583/251‑1 (MQ=255)                                                                             agtGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTAGTTATTTAGAGATCTGTTCTATTGGGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGAGCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATTAGGGGTTATACACAACt                                                                                                                                                                  <  2:24514/249‑1 (MQ=255)                                                                                gAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAATACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCaa                                                                                                                                                               <  2:577440/251‑1 (MQ=255)                                                                                          ccGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGaaca                                                                                                                                                     <  2:202736/251‑1 (MQ=255)                                                                                                              tGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAAc                                                                                                                                 >  1:190799/1‑251 (MQ=255)                                                                                                                         gaAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGAt                                                                                                                      <  2:241017/251‑1 (MQ=255)                                                                                                                               ccTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGAGCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAGGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGc                                                                                                                <  2:395501/251‑1 (MQ=255)                                                                                                                                     aTCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTg                                                                                                          >  2:355058/1‑251 (MQ=255)                                                                                                                                            gTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTGATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGt                                                                                                   <  1:334812/251‑1 (MQ=255)                                                                                                                                                   aaaGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTGATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGAGCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCcac                                                                                            <  1:246818/251‑1 (MQ=255)                                                                                                                                                     agatgaTCTATTGATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATACGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAg                                                                                          <  2:310033/247‑1 (MQ=255)                                                                                                                                                     agaagaTCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCAGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCcac                                                                                            >  1:541702/1‑249 (MQ=255)                                                                                                                                                          atatatttattTAGATATCTGTACTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAATTACCGGTTGATCCAAGATTCCTGACAGAGTTATCCACAGt                                                                                         >  2:385903/4‑247 (MQ=255)                                                                                                                                                                 tatttaGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACg                                                                               >  1:549159/1‑250 (MQ=255)                                                                                                                                                                 tatttaGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACg                                                                               >  1:488401/1‑250 (MQ=255)                                                                                                                                                                              gTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTGTAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACt                                                                    <  1:533371/248‑1 (MQ=255)                                                                                                                                                                               ttCTATTGTGACCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGCAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATACACAGTAGATCGCACGATCTGTATACTTAtt                                                                >  2:292739/1‑251 (MQ=255)                                                                                                                                                                                tCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTAttt                                                               >  1:46796/1‑251 (MQ=255)                                                                                                                                                                                          tctcTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAAtt                                                      >  1:155868/1‑250 (MQ=255)                                                                                                                                                                                             ctTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAAtt                                                      <  1:122547/247‑1 (MQ=255)                                                                                                                                                                                                attaGGCTCGCCCTGCCCCGCGGATAGACA‑GGATCGGGCTTTTAAGATCACTAACCTGAAAAGGATCCTTAACTGTGAATGATCGGCGATCATGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGCCAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCAc                                               >  2:55532/1‑251 (MQ=255)                                                                                                                                                                                                    ggATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATc                                           >  2:141166/1‑251 (MQ=255)                                                                                                                                                                                                      aTCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGAAAAGCATCATTAACTGTGACTGATCGGTGATCATGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCCAACCCTGAACAACAGTTGTTCTTTGGCTAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGCTCGCACGATCTGTATCCTTATTTGAGTAACTTAACCCACGATccc                                         >  1:371365/1‑251 (MQ=255)                                                                                                                                                                                                             tGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAATAACATGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCAtt                                  >  1:541434/1‑251 (MQ=255)                                                                                                                                                                                                                      gATA‑ACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTaa                                                    <  2:123168/224‑1 (MQ=255)                                                                                                                                                                                                                                        ttttAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTc         <  2:62155/250‑1 (MQ=255)                                                                                                                                                                                                                                             aGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATc   <  1:172554/251‑1 (MQ=255)                                                                                                                                                                                                                                               aTCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCa  >  2:120221/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     CCCGCCAATGATGATGACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATA‑ACAAGGATCCGGCTTTTAAGATCAACAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCA  >  NC_000913/3925592‑3926078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑