Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,770,539	C→T	V281M (GTG→ATG)	yibH ←	inner membrane protein YibH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,770,539	0	C	T	100.0%	214.8 / NA	59	V281M (GTG→ATG)	yibH	inner membrane protein YibH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/28);  total (31/28)

TTCCGCATCACCGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACACCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCT  >  NC_000913/3770295‑3770784                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ttCCGCATCACCGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACtt                                                                                                                                                                                                                                                  <  1:312514/250‑1 (MQ=255) ttCCGCATCACCGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACAACAGGAAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTc                                                                                                                                                                                                                                                 >  1:312990/1‑251 (MQ=255)    ctcATCACCGAAACATGGATGAAACGGTAGGAGTAAACCGCCACCTGGGCCTAGCTGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACAGTTAATGATTGCAATACACCCTGCGCCTGATAAGACCCGCCTGGCACGAAAGGTAAAATACTAGTCAGTTTGCCGTGCAACACCTGCCCAGGTAGCTCGTTAAAAATCACTTCCGc                                                                                                                                                                                                                                              >  2:551632/3‑251 (MQ=255)       aTCACCGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGcatc                                                                                                                                                                                                                                           >  2:125069/1‑251 (MQ=255)         ccccGAAACGTGGCTTAAATGGTCCTAGTAAACAGCCACATGGGCGTGTATGCCGTCGGGTAAGGCGTCGATATCATCGTTAGGGTCCAGTCCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGTCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACGTGCCCAGGTAGCGCGTTAAACATCACTTCCGcatcat                                                                                                                                                                                                                                         <  2:156729/249‑1 (MQ=255)           ccGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCg                                                                                                                                                                                                                                       <  1:132168/251‑1 (MQ=255)              aaaCATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGcc                                                                                                                                                                                                                                     >  1:570713/1‑250 (MQ=255)                aCATGGATGAAATGGTCGGAGTAAACAGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAgg                                                                                                                                                                                                                                  >  1:511457/1‑251 (MQ=255)                  aTGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGtt                                                                                                                                                                                                                                <  2:225599/251‑1 (MQ=255)                  aTGGCTGAAAGGGTCGGAGTAAACCGCCGCCAGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTGAGGGTCAAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGAATGCAATACCCCCTGCGCCTGATAAGAACCGCCGGGCGCGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGGTAAACATCACTTCCGCTTCATCGCCAGGtt                                                                                                                                                                                                                                <  2:460654/251‑1 (MQ=255)                     gCTGAAATTGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGGCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCACTACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTTAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCCTCATCGCCAGGTTTc                                                                                                                                                                                                                              >  2:301524/1‑250 (MQ=255)                        gAAATGGTCGGAGAAAACAGGAACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGAc                                                                                                                                                                                                                          >  1:117294/1‑251 (MQ=255)                               gcGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAg                                                                                                                                                                                                                   <  2:232243/250‑1 (MQ=255)                                 ggAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCg                                                                                                                                                                                                                 >  1:463348/1‑251 (MQ=255)                                     tAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACCGCGAGtt                                                                                                                                                                                                             >  1:493623/1‑251 (MQ=255)                                      aaaCCGCCACCTGGTCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACTCCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCGGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGttt                                                                                                                                                                                                            <  2:487860/251‑1 (MQ=255)                                                  gggCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAAtt                                                                                                                                                                                                  <  2:135863/249‑1 (MQ=255)                                                   ggCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTggg                                                                                                                                                                                               >  2:523688/1‑251 (MQ=255)                                                           tGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAAtt                                                                                                                                                                                       >  2:110085/1‑251 (MQ=255)                                                                    gTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCTTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGtttt                                                                                                                                                                              <  1:381583/251‑1 (MQ=255)                                                                      aaGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTACCAGCACAACGTCCTTGCCGGGCACGACCGTTACTGGTTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGCAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTCAACATCCCTTCCGCATCATCGCCACATTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTg                                                                                                                                                                            >  2:276393/1‑251 (MQ=255)                                                                                   catcGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGa                                                                                                                                                                 >  2:252561/1‑249 (MQ=255)                                                                                   catcGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAg                                                                                                                                                               <  2:581153/251‑1 (MQ=255)                                                                                    atcGTTAGGGTCCAGTTCAATGGTTCCCAGCACCCCGTCCGTGCCGGGCCCGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGTGGATGAAGa                                                                                                                                                              <  2:117294/251‑1 (MQ=255)                                                                                                cAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGAc                                                                                                                                                  <  2:229794/251‑1 (MQ=255)                                                                                                cAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGAc                                                                                                                                                  <  2:35703/251‑1 (MQ=255)                                                                                                                aGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGc                                                                                                                                  >  2:507300/1‑251 (MQ=255)                                                                                                                         tccgtGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGt                                                                                                                         >  2:442698/1‑251 (MQ=255)                                                                                                                            gtGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTAcc                                                                                                                      <  1:20130/251‑1 (MQ=255)                                                                                                                               ccGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTgg                                                                                                                   <  2:25278/251‑1 (MQ=255)                                                                                                                                 gggCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGc                                                                                                                 >  2:183385/1‑251 (MQ=255)                                                                                                                                  ggCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCAACATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCg                                                                                                                <  2:401372/251‑1 (MQ=255)                                                                                                                                    cACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCggg                                                                                                              >  2:147998/1‑250 (MQ=255)                                                                                                                                      cGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCTTCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATc                                                                                                            >  2:406366/1‑251 (MQ=255)                                                                                                                                       gACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCa                                                                                                           <  1:539708/251‑1 (MQ=255)                                                                                                                                            ttAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTAcc                                                                                                      >  1:178515/1‑251 (MQ=255)                                                                                                                                              aaTGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTg                                                                                                    >  1:177701/1‑251 (MQ=255)                                                                                                                                               aTGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTg                                                                                                    >  2:310902/1‑250 (MQ=255)                                                                                                                                                  aTTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGt                                                                                                 >  1:525862/1‑250 (MQ=255)                                                                                                                                                   ttGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTa                                                                                                >  2:554750/1‑250 (MQ=255)                                                                                                                                                     gCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAAc                                                                                              <  1:543867/250‑1 (MQ=255)                                                                                                                                                       aaTACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGt                                                                                            >  2:170234/1‑250 (MQ=255)                                                                                                                                                         tACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAAGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGc                                                                                         >  2:277037/1‑251 (MQ=255)                                                                                                                                                                tgtgcCTGATAAGAACCACCTGGCACGACCGGTAACACACTAGTCAGTTTGCCGTGAAACCCCCGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGCGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGcc                                                                                  >  2:280060/4‑250 (MQ=255)                                                                                                                                                                          aaGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGcgcgcg                                                                         <  1:245148/250‑1 (MQ=255)                                                                                                                                                                                cGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGAc                                                                   >  1:191599/1‑250 (MQ=255)                                                                                                                                                                                       cACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTGCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCt                                                           <  2:129885/251‑1 (MQ=255)                                                                                                                                                                                             aGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGt                                                     <  1:119162/251‑1 (MQ=255)                                                                                                                                                                                              ggTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGtt                                                    <  1:110085/251‑1 (MQ=255)                                                                                                                                                                                                   aaTACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATAtt                                               >  1:85301/1‑251 (MQ=255)                                                                                                                                                                                                            cAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTc                                        >  1:447682/1‑249 (MQ=255)                                                                                                                                                                                                                  gCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCGCAAGGTTATATTTTGCTTCAGTAAGt                                 <  1:502125/250‑1 (MQ=255)                                                                                                                                                                                                                           cacCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAAGGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTa                       <  1:238205/251‑1 (MQ=255)                                                                                                                                                                                                                            acCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTa                       >  2:102426/1‑250 (MQ=255)                                                                                                                                                                                                                                gctcAGGTAGCGCGTTAAACATCCCGTCCGCATCATCGCCAGGTTTCAGACGTAGCAGCGAGTTTTGCCGAAAGTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATTTACCTGGGAGGATCAGTACCTGAGTAATTTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGGTATATTTCGCTTCAGTAAGCTGCGCTCTTTAGCTc                  <  2:290023/248‑1 (MQ=255)                                                                                                                                                                                                                                    aGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTcaca               >  1:457943/1‑250 (MQ=255)                                                                                                                                                                                                                                     ggTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAAt             <  1:47810/251‑1 (MQ=255)                                                                                                                                                                                                                                      gTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATc            <  1:310458/251‑1 (MQ=255)                                                                                                                                                                                                                                                aaaCATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCt  <  2:17715/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      TTCCGCATCACCGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACACCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCT  >  NC_000913/3770295‑3770784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑