Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,520,507	G→A	P66L (CCC→CTC)	hofQ ←	DNA utilization protein HofQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,520,507	0	G	A	100.0%	245.6 / NA	67	P66L (CCC→CTC)	hofQ	DNA utilization protein HofQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/34);  total (33/34)

GGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGCAGTTT  >  NC_000913/3520263‑3520750                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    ggTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACAt                                                                                                                                                                                                                                               >  2:267817/1‑251 (MQ=255)            cTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGACCGCGCCTGCTCCGCCCCCTGGCGGGCGGTATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCCGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACCTCTGTTAGATGt                                                                                                                                                                                                                                    >  2:67402/1‑251 (MQ=255)               tGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCCGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTaa                                                                                                                                                                                                                                  <  2:217241/250‑1 (MQ=255)                          ggCTTCTCCCCCGCTTGCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTACGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTAcc                                                                                                                                                                                                                      <  2:445955/250‑1 (MQ=255)                          aGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTAcc                                                                                                                                                                                                                      <  2:87055/251‑1 (MQ=255)                           gCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCCGGGTTAGACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTAGCAGTCCGGCGCGGTTCACTACAGTTTGTAGTGCCTGCTTCCATAGAACATCTGTTAGATTTATCTACTCCGTACCg                                                                                                                                                                                                                     <  2:291743/251‑1 (MQ=255)                                     cGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTg                                                                                                                                                                                                           <  2:14219/251‑1 (MQ=255)                                                        gcgTCGGCGTATTGCAGTGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCa                                                                                                                                                                                         >  1:325611/1‑250 (MQ=255)                                                        gcgTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCa                                                                                                                                                                                         >  1:460650/1‑250 (MQ=255)                                                        gcgTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGAAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTCGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCa                                                                                                                                                                                         >  1:325577/1‑250 (MQ=255)                                                              gCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGGTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTtctc                                                                                                                                                                                  <  1:254754/251‑1 (MQ=255)                                                                  aTTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGt                                                                                                                                                                              >  1:244486/1‑251 (MQ=255)                                                                     gCAGGGTTATACTGCTATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGGCGGGCGACACGACCAGGTTCAACTTCTCCTGTTca                                                                                                                                                                           <  1:530142/251‑1 (MQ=255)                                                                     gCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTc                                                                                                                                                                            <  1:424978/250‑1 (MQ=255)                                                                         ggTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagcca                                                                                                                                                                       <  2:299021/251‑1 (MQ=255)                                                                                             gCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCt                                                                                                                                                   <  2:99692/251‑1 (MQ=255)                                                                                                         ccTGCGCCCGCGACTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCa                                                                                                                                       <  2:153695/251‑1 (MQ=255)                                                                                                            gcgcCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATc                                                                                                                                     <  1:283867/250‑1 (MQ=255)                                                                                                             cgcCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATcca                                                                                                                                   <  2:419516/251‑1 (MQ=255)                                                                                                             cgcCCGCGCCTGCTCCGCCGCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTGCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACTCCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACTCCTGAGCCACCGGAACGTCATcca                                                                                                                                   <  2:347655/251‑1 (MQ=255)                                                                                                              ccccGCGCCTTCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCCGTCAATGTAATGCACTGATAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCTGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTTTTAGATGTAACGACACCGTACCGCTGCCGTCTGGCGACCCGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATccac                                                                                                                                  <  2:440178/250‑1 (MQ=255)                                                                                                                    gcCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATc                                                                                                                              >  2:544821/1‑249 (MQ=255)                                                                                                                       tGCTCCGCCTCCTGGCGGGCGATATTGTTCTTCTGCCAGGCAATGGACTGCACTGAGCGCATGTTGCCTTCCTGCCGCGTTATAAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGt                                                                                                                         >  2:244627/1‑251 (MQ=255)                                                                                                                        gCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTc                                                                                                                        <  1:360976/251‑1 (MQ=255)                                                                                                                           ccGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATTGAATGCACTGATAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACt                                                                                                                     <  1:228773/251‑1 (MQ=255)                                                                                                                           ccGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCAc                                                                                                                      <  1:550914/250‑1 (MQ=255)                                                                                                                           ccGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCAc                                                                                                                      <  1:483979/250‑1 (MQ=255)                                                                                                                             gCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACttt                                                                                                                   >  2:421744/1‑251 (MQ=255)                                                                                                                              cctcctGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAAGGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGGCACtttt                                                                                                                  <  2:525185/251‑1 (MQ=255)                                                                                                                                 cctGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTg                                                                                                                <  2:244486/250‑1 (MQ=255)                                                                                                                                        ggcgATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTg                                                                                                        <  2:32974/251‑1 (MQ=255)                                                                                                                                          cgATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTgcc                                                                                                      <  2:196175/251‑1 (MQ=255)                                                                                                                                                 gTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGt                                                                                                >  1:272421/1‑250 (MQ=255)                                                                                                                                                  ttATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTAc                                                                                              >  2:344474/1‑251 (MQ=255)                                                                                                                                                  ttATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTAc                                                                                              >  2:10628/1‑251 (MQ=255)                                                                                                                                                      tCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGcc                                                                                           <  2:419538/250‑1 (MQ=255)                                                                                                                                                            aGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGCACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCAGGGTATc                                                                                    >  1:131593/1‑251 (MQ=255)                                                                                                                                                             ggCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCa                                                                                   <  2:129545/251‑1 (MQ=255)                                                                                                                                                                aaTGGAAGGCACTGAACGAATGTTGCCTACCTGCCGAGTTATCAGTCCAGCGCTTTTCAATCCAGTTTGTAGTGCCTGCTTCCAGAGAACATATGTTAGAAGTAACGACACCGTACAGCTGACGTCTGGCGAAACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACATGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCTGCATTGCAGCCTGTACGCCGGGTATCatc                                                                                 >  2:295790/1‑248 (MQ=255)                                                                                                                                                                 aTGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCAt                                                                               <  1:155164/251‑1 (MQ=255)                                                                                                                                                                   ggAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATc                                                                              >  1:198808/1‑250 (MQ=255)                                                                                                                                                                       tGCACTGAGAGAATGTTGCCTTCCTGACGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAAc                                                                           >  1:465364/1‑249 (MQ=255)                                                                                                                                                                          aCTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTGTTCACTACATGTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGACCGTCATCCACCATCAGCGTCACTTTTTGCGGCGTTGCAGCCTGTACGCCGGGTATCAGCATCAACagtag                                                                      <  2:53904/251‑1 (MQ=255)                                                                                                                                                                                 gaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCt                                                               >  2:280536/1‑251 (MQ=255)                                                                                                                                                                                  aaTGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCt                                                               >  2:75489/1‑250 (MQ=255)                                                                                                                                                                                     gTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATcc                                                           >  1:380839/1‑251 (MQ=255)                                                                                                                                                                                       tGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCAt                                                         >  1:543442/1‑251 (MQ=255)                                                                                                                                                                                                   gcgTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGt                                             <  1:18716/251‑1 (MQ=255)                                                                                                                                                                                                   gcgTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGAATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGt                                             <  1:267817/251‑1 (MQ=255)                                                                                                                                                                                                    cgTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGt                                             >  2:549725/1‑250 (MQ=255)                                                                                                                                                                                                       tATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTAtct                                         >  1:125349/1‑251 (MQ=255)                                                                                                                                                                                                        aTCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTAtctc                                        >  2:216465/1‑251 (MQ=255)                                                                                                                                                                                                        aTCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTAtct                                         <  2:464182/250‑1 (MQ=255)                                                                                                                                                                                                          cAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCt                                      >  1:161020/1‑251 (MQ=255)                                                                                                                                                                                                                ggCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGATCGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGt                                <  1:344474/251‑1 (MQ=255)                                                                                                                                                                                                                ggCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGt                                >  2:114823/1‑251 (MQ=255)                                                                                                                                                                                                                  cgcTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTg                              >  2:149813/1‑251 (MQ=255)                                                                                                                                                                                                                        tCACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATTTAACGACACCGTACCGTTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCGTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCcaca                         <  1:381281/250‑1 (MQ=255)                                                                                                                                                                                                                         cACTACAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAAc                       <  2:201500/251‑1 (MQ=255)                                                                                                                                                                                                                             aCAGTTTGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCa                     <  2:240801/249‑1 (MQ=255)                                                                                                                                                                                                                             aCAGTCTGTCGTGACTGCTTCCAGAGAACATCTGTTAGATGAAACGACACCGTACCGCTGACGTCTGGCGACCCGACCAGGTTCAACTTCTCCTGCTCAGCCAGCGCCTGCAACACCTGAGCCACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGCCATCAGCATCACCAGTAGTTCGGCCATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCAtt                   >  2:338109/1‑251 (MQ=255)                                                                                                                                                                                                                                  ttGTAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGc              >  1:159862/1‑251 (MQ=255)                                                                                                                                                                                                                                     tAGTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTAACGCTGACGTCTGGCGACACGCCCAGGTTCAACTTCTCTTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATACCCCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCgt            >  1:58388/1‑249 (MQ=255)                                                                                                                                                                                                                                       gTGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACAGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCAGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTc         >  1:580307/1‑251 (MQ=255)                                                                                                                                                                                                                                        tGCCTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGtttt        >  1:521322/1‑249 (MQ=255)                                                                                                                                                                                                                                           cTGCTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGc       >  2:11160/1‑249 (MQ=255)                                                                                                                                                                                                                                              cTTCCAGAGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGCAGttt  >  1:34470/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    GGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGCAGTTT  >  NC_000913/3520263‑3520750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑