Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,439,485	C→T	E9K (GAG→AAG)	yhdN ←	DUF1992 domain‑containing protein YhdN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,439,485	0	C	T	100.0%	120.9 / NA	34	E9K (GAG→AAG)	yhdN	DUF1992 domain‑containing protein YhdN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/20);  total (14/20)

ACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCAC  >  NC_000913/3439239‑3439725                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 aCAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCACGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATCTGGCGCTTTGcc                                                                                                                                                                                                                                              >  1:527059/1‑251 (MQ=255)   aaTCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGccc                                                                                                                                                                                                                                             >  2:222438/1‑250 (MQ=255)               aCCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTaa                                                                                                                                                                                                                                <  1:306387/251‑1 (MQ=255)                      gttatTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTTAGAATCATCATCCAGTATCAATGGTTCGCCGCTTCCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACAt                                                                                                                                                                                                                          <  1:386722/248‑1 (MQ=255)                                     cGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGa                                                                                                                                                                                                           <  2:53096/250‑1 (MQ=255)                                     cGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAACACTCCAGGGGa                                                                                                                                                                                                           <  1:466899/250‑1 (MQ=255)                                        gACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGaa                                                                                                                                                                                                       <  1:97492/251‑1 (MQ=255)                                               aCCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGTCGCTACCTGCCAGGTTATCAAACTCACCTGTCGCGTGCGCTTCTGCGATATGGCGCTTGGCCCACATGTCAAGTAACCACATAAATACTCCAGGGGATGAACaaaaa                                                                                                                                                                                                 <  1:338564/250‑1 (MQ=255)                                                                                   ccGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACggg                                                                                                                                                            >  2:383668/1‑251 (MQ=255)                                                                                   ccGTTGCTCAAGGTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGAtactac                                                                                                                                                               <  1:461616/248‑1 (MQ=255)                                                                                    cGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGt                                                                                                                                                           >  1:419880/1‑251 (MQ=255)                                                                                            aaGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATaaaaa                                                                                                                                                    >  2:379548/1‑250 (MQ=255)                                                                                              gTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAAc                                                                                                                                                 >  1:52583/1‑251 (MQ=255)                                                                                                                  gCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGttttttt                                                                                                                             <  1:468160/251‑1 (MQ=255)                                                                                                                           aGCAAGCGCTACCCCACACGTAATTCCGTTGGCCCGTGAGAATCATCATCCAGTATAAATGGTTCGCCGCAACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATAAACAAAAAGAGTACAGCTAGTGTAGATAATTGGGGATACTACGGGTATAAAAAAACCCGCCGTGGCGGGTTTTTTTACGTTGCtg                                                                                                                    <  2:477617/251‑2 (MQ=255)                                                                                                                             cAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTc                                                                                                                   >  1:379163/1‑250 (MQ=255)                                                                                                                                  gATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTTCtttcgatt                                                                                                              >  1:320645/1‑244 (MQ=255)                                                                                                                                         cGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgc                                                                                                       >  1:39035/1‑250 (MQ=255)                                                                                                                                                 aTTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTCCCTGCCAGTTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGTCGCTTTGCCCACTGGTCAATTAACCACATAAATGCGCCAGGGGAGGAACAAAAAGAGTACAGCTATTGGAGAGAAGGGGGGATACTACGGGGATAAAAAAACCCGCCGGGGCGGGTTGTTTTACGTTGCTTCAGATTACTCGGCATCAGCTTc                                                                                              <  2:206821/251‑1 (MQ=255)                                                                                                                                                    ccGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAACAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGGTTTTTTACGTTGCTTCCGATTACTCTGCAGCAGCTTCTGc                                                                                           >  2:447519/1‑251 (MQ=255)                                                                                                                                                       gTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCCCCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCttt                                                                                        <  1:96276/251‑1 (MQ=255)                                                                                                                                                         ggCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtct                                                                                      <  2:180390/251‑1 (MQ=255)                                                                                                                                                         ggCACGGGAGAATCATCTACCAGTACCAATGGTCCGCCGCTCCCTGCCAGGTTATCAAACTCCCCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATCCTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtct                                                                                      <  1:323226/251‑1 (MQ=255)                                                                                                                                                           cACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGGATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTctct                                                                                    <  1:83719/251‑1 (MQ=255)                                                                                                                                                             cGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTg                                                                                   >  1:535348/1‑250 (MQ=255)                                                                                                                                                                         catcCCGTATCAATGGTTCGCCGCTACCTGCCAGACTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCAACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGCTTTTTTACGATGCTTCCGATTACTCTGCAGCAGCATCCGCTTTCTCTGAAAGATCAAcc                                                                        >  2:72291/1‑249 (MQ=255)                                                                                                                                                                            ccAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCGCTGAACGATCAACCAGCt                                                                    <  2:93224/250‑1 (MQ=255)                                                                                                                                                                                                  ccatgacaggttatcaaccgagccTTTCGCGTGCGCTTCTGCTAAAGGGCGCTTTCCCCACTGTTCAAGTAACCACATAAATCCTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGcgcgctg                                             <  2:148289/229‑4 (MQ=255)                                                                                                                                                                                                      gCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGAGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGc                                         <  1:383668/251‑1 (MQ=255)                                                                                                                                                                                                                 aaaCTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAg                              <  1:319677/251‑1 (MQ=255)                                                                                                                                                                                                                            tCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCa                    <  1:492355/250‑1 (MQ=255)                                                                                                                                                                                                                               cTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTTTCGCCTGCACGGAAGCCACACTTCCGaa                 >  2:425932/1‑250 (MQ=255)                                                                                                                                                                                                                                          gCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAgtgt        <  2:57300/248‑1 (MQ=255)                                                                                                                                                                                                                                             atatGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGGTTTTTTACGTTTCCTCAGATTACTCAGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCCACTCCATGTAAGCCATCCGCGCCTTGTCCCCTTCACGGAAGCCACCCTTTAGAATACGACTGTAaccac  >  2:566822/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 ACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCAC  >  NC_000913/3439239‑3439725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑