Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,769,820	T→C	S40P (TCA→CCA)	yfjR →	putative DNA‑binding transcriptional regulator YfjR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,769,820	0	T	C	100.0%	217.2 / NA	61	S40P (TCA→CCA)	yfjR	putative DNA‑binding transcriptional regulator YfjR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/28);  total (33/28)

GTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGC  >  NC_000913/2769584‑2770066                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       gTTACATCCGGCTTTTGAAGAGATCAAAAATAGCTTCTGCGTTGTCCATACCCTGTCGGCACACCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGACGCCATGACCGGCTGGCTGTCCGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGACACGTGGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTg                                                                                                                                                                                                                                          >  2:550402/1‑251 (MQ=18)          ggCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGAt                                                                                                                                                                                                                                 <  1:583060/251‑1 (MQ=255)                 gCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTg                                                                                                                                                                                                                          <  1:200275/251‑1 (MQ=255)                                 gTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCt                                                                                                                                                                                                          >  1:401252/1‑251 (MQ=255)                                       gCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCAGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGAc                                                                                                                                                                                                      <  2:397856/249‑1 (MQ=255)                                       gCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAAGCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCt                                                                                                                                                                                                    <  2:49666/251‑1 (MQ=255)                                               cATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATc                                                                                                                                                                                            >  2:366429/1‑251 (MQ=255)                                                  aCCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGt                                                                                                                                                                                         >  1:479186/1‑251 (MQ=255)                                                         ccGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGGGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGAt                                                                                                                                                                                   <  1:79743/250‑1 (MQ=255)                                                               cccTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCg                                                                                                                                                                            <  1:238970/251‑1 (MQ=255)                                                                 ctctTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGCGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCt                                                                                                                                                                          >  2:29140/1‑251 (MQ=255)                                                                     ttAAATTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTAc                                                                                                                                                                       <  2:131842/250‑1 (MQ=255)                                                                      tAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGc                                                                                                                                                                     >  2:505786/1‑251 (MQ=255)                                                                       aaaGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGGATCAGTCCGGATACTGCCGCTTACGc                                                                                                                                                                     >  1:2647/1‑250 (MQ=255)                                                                           tAATCACATCATTTTCAGTCCGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCActgc                                                                                                                                                                >  2:361356/1‑251 (MQ=255)                                                                                 catcatTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAg                                                                                                                                                          <  1:265829/251‑1 (MQ=255)                                                                                      ttttCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGCTACTGCCGCTTACGCACTGCTGGCAGTg                                                                                                                                                        >  2:303027/1‑248 (MQ=255)                                                                                       tttCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTg                                                                                                                                                        >  1:486195/1‑247 (MQ=255)                                                                                         tcagtcagTTAACTTTCCTGGAGAATCTCTCATGACACAGTCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGGGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGATCGACTGATGTATCTGGACCTGTAGTTTCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATg                                                                                                                                                   <  1:569209/250‑1 (MQ=255)                                                                                           agtcagtTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGc                                                                                                                                                  >  1:410554/1‑249 (MQ=255)                                                                                           agtcagtTAACTTTCATGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCCGTGAGATGc                                                                                                                                                  >  2:335293/1‑249 (MQ=255)                                                                                                   aaCTTTCCTGGAGATTCTCTCATGACACAGGCAGACCGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCGGGACCTGGAGTAGCAGTCCGGATACTGCCGCATACGTACTGCTGGCAGTGAGATGCAGATGGTGcc                                                                                                                                        <  2:239155/251‑1 (MQ=25)                                                                                                   aaCTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGc                                                                                                                                         <  1:455368/250‑1 (MQ=255)                                                                                                    aCTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGccc                                                                                                                                       <  1:550430/251‑1 (MQ=255)                                                                                                       ttCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGGATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGGGAGATGCCGATGGTGCCCGAc                                                                                                                                    >  2:302179/1‑251 (MQ=255)                                                                                                           tGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCCGATGGTGCCCGACGTGc                                                                                                                                >  1:321055/1‑251 (MQ=255)                                                                                                            ggAGAATCTCTCATGACACCGGAAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTAACTGATAATCAGCGGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGCGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGGTGTATCTGGACCTGGAGGATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGCGCt                                                                                                                               >  2:59630/1‑251 (MQ=25)                                                                                                                 aTCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCt                                                                                                                          >  1:581067/1‑251 (MQ=255)                                                                                                                     ctcATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCttt                                                                                                                        <  1:302179/249‑1 (MQ=255)                                                                                                                          gACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCCGAGCGCACGCTGCGGCGTGATTTTCGTGAACGAATGATGTATCTGGCCCTGGAGTATCAGTCCGGAGACTGACGCTTACGCCCTGCTGGCAGTGCGATGCAGATGGTGCCCGACGTTCTTATCTTTGccccac                                                                                                                 >  2:31430/1‑248 (MQ=21)                                                                                                                              caGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGc                                                                                                               >  2:271947/1‑249 (MQ=255)                                                                                                                              caGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAg                                                                                                             <  1:96661/251‑1 (MQ=255)                                                                                                                               aGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGc                                                                                                            >  1:120738/1‑251 (MQ=255)                                                                                                                                 gCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCCGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCgg                                                                                                          >  1:383779/1‑251 (MQ=255)                                                                                                                                         ccgccATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTTCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTgtccc                                                                                                  >  1:251207/1‑247 (MQ=255)                                                                                                                                         ccgccATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCg                                                                                                  <  1:433140/251‑1 (MQ=255)                                                                                                                                           gccATGACCGGCTGGCTGTCAGGCTGTCCCTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGt                                                                                                                                                                                               >  1:473647/1‑156 (MQ=255)                                                                                                                                           gccATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCggg                                                                                                >  1:187795/1‑251 (MQ=255)                                                                                                                                           gccATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGGGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGt                                                                                                                                                                                               <  2:473647/156‑1 (MQ=255)                                                                                                                                                  ccGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTc                                                                                         <  2:582333/251‑1 (MQ=255)                                                                                                                                                            gtcaggctgtcaCTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTg                                                                                >  1:109728/1‑250 (MQ=255)                                                                                                                                                            gtcaggctgtcaCTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTg                                                                                >  1:8179/1‑250 (MQ=255)                                                                                                                                                                          gATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTg                                                                  <  1:127713/250‑1 (MQ=255)                                                                                                                                                                                 aGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCAct                                                          <  2:479186/251‑1 (MQ=255)                                                                                                                                                                                 aGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGCCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGCCCGCCGTCTGGTGAATGCAct                                                          >  1:505096/1‑251 (MQ=255)                                                                                                                                                                                   ccGTCTGGTTGCGGCTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGCCCTTGACCGCCGTCTGGTGAATGCActgc                                                        >  2:378988/1‑251 (MQ=255)                                                                                                                                                                                         ggTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGAtg                                                   <  1:335293/250‑1 (MQ=255)                                                                                                                                                                                            ggCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCg                                               <  2:198779/250‑1 (MQ=255)                                                                                                                                                                                               gggTGAAACGTTCAGCGTGCGCAAACTGGCGGCTGAGCTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTTAACGACTGATGTATCTGGACCTGGTGTATCAGTCCGGATACTGCCGCTTACGCACTGCCGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGTCTTTTTCCCGGCCTTGACCGCCGTCTGGGGAATGCACTGCTGATGTGCgctg                                            >  2:287049/1‑248 (MQ=255)                                                                                                                                                                                                 gTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTTCGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATg                                            >  2:399387/1‑249 (MQ=255)                                                                                                                                                                                                        gTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCt                                   <  1:333246/251‑1 (MQ=255)                                                                                                                                                                                                              cgtacgaaaaCTGGCGGCTGAGTTTGACGTGCGAGTGCGAACGCTGAGGCGTGATTTTCGTGAACGTCTGATGTAGCTGGACCTGGAGTATCAGTCCGGATATTGCCGCTTACGCACTGCTGGCAGAGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGTGCGGGCCGGGATTTGTCCCGGCCTTGACCGCCGTCTGGTTAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGa                             <  2:397083/244‑1 (MQ=255)                                                                                                                                                                                                                 gcgcAAACTTGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGGGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTGTCCCGGCCTTTACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGATTCTCCCTGCGGGAt                            >  2:454011/1‑249 (MQ=38)                                                                                                                                                                                                                   gcAAACTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGGGATAGCa                        <  2:353587/251‑1 (MQ=255)                                                                                                                                                                                                                      aaCTGGCGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCa                     <  1:285720/251‑1 (MQ=255)                                                                                                                                                                                                                      aaCTGGAGGCTGAGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCa                     <  1:366429/251‑1 (MQ=255)                                                                                                                                                                                                                              gctgatgttGGCGTGCCAGTGCGCACGCTGGGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATcc             <  2:476616/244‑1 (MQ=255)                                                                                                                                                                                                                                  aGTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGtt         <  1:110874/251‑1 (MQ=255)                                                                                                                                                                                                                                   gTTTGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTc        >  1:139065/1‑251 (MQ=255)                                                                                                                                                                                                                                    tttGGCGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGTCGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTcc       >  2:392588/1‑251 (MQ=255)                                                                                                                                                                                                                                         cGTGCCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGc  >  2:309137/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       GTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGC  >  NC_000913/2769584‑2770066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑