Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	431,304	IS1 (–) +9 bp	coding (702‑710/885 nt)	tsx ←	nucleoside‑specific channel‑forming protein Tsx

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	69 (1.150)	59/494	0.1	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 431312	0 (0.000)					coding (702/885 nt)	tsx	nucleoside‑specific channel‑forming protein Tsx
*	?	NC_000913	= 258675	NA (NA)	66 (1.100)	62/494	0.1	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	431304 =	0 (0.000)					coding (710/885 nt)	tsx	nucleoside‑specific channel‑forming protein Tsx

AAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGA  >  NC_000913/257902‑258156       |                                                                                                                                                                                                                                                         aaGGCAGGTAATGCCTCCAACTTATTGATCGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCCGCCATCCGTCATCCATATCACCACGTCAAAgg      >  2:76440/1‑251 (MQ=2)      aggtaATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGa  >  1:228353/1‑250 (MQ=11)       |                                                                                                                                                                                                                                                         AAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGA  >  NC_000913/257902‑258156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.