Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,975,224	G→A	A34V (GCG→GTG)	cheA ←	chemotaxis protein CheA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,975,224	0	G	A	100.0%	69.0 / NA	21	A34V (GCG→GTG)	cheA	chemotaxis protein CheA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/15);  total (6/15)

CCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTG  >  NC_000913/1974991‑1975454                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       ccGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAAcc                                                                                                                                                                                                                       <  1:358876/251‑1 (MQ=255)                        gCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTTCATGTCAGc                                                                                                                                                                                                <  2:158049/251‑1 (MQ=255)                                  gTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCg                                                                                                                                                                                      <  1:202162/251‑1 (MQ=255)                                       tGCATGATGTCCGTCGTTTCCAAAAACAGATTGATAATGTCGGTGTGGAGTTGCATCTCTCCTCGTCTGGCTTCATCGAGCAGGTTTTCCAGCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTGCGGCATCTGGCACTTCCGTCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTcc                                                                                                                                                                                   <  2:142952/249‑1 (MQ=255)                                              tgTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAACCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTtcatc                                                                                                                                                                           >  1:205348/1‑250 (MQ=255)                                                                      tGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCt                                                                                                                                                   <  1:5745/250‑1 (MQ=255)                                                                               cGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGACTGATAAAAATCGCTTATATCCATg                                                                                                                                         <  2:204130/251‑1 (MQ=255)                                                                                           gCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCa                                                                                                                             <  2:86325/251‑1 (MQ=255)                                                                                                               tCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGc                                                                                                          <  1:15853/250‑1 (MQ=255)                                                                                                                   cgggCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAAGCGCTTATATCCATGCTCACGCTGTCACCCCGGTTCGGCTGATGGCATtg                                                                                                      <  2:259777/247‑1 (MQ=255)                                                                                                                         ggTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTTCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAac                                                                                               <  1:183599/251‑1 (MQ=255)                                                                                                                          gTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAac                                                                                               >  1:316003/1‑250 (MQ=255)                                                                                                                                        gCGTGGTTTCCTGCAAAACGCTGAGGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGc                                                                                <  2:296403/251‑1 (MQ=255)                                                                                                                                                                         ttCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCTTCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTc                                               >  2:243894/1‑251 (MQ=255)                                                                                                                                                                           ccTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCAt                                             >  1:285643/1‑251 (MQ=255)                                                                                                                                                                           ccTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCAt                                             <  2:260602/251‑1 (MQ=255)                                                                                                                                                                              gCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTc                                           <  1:18842/250‑1 (MQ=255)                                                                                                                                                                                                 gggCAGCCCGAAAGATGGCATTCAATTGTTCTGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGGTGTCACCGCGGGTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGGTTTTCCAGGGCGCTTACTGGCTCATTCGGGCTTTCGGGGTTTTCATg                       <  2:297036/251‑1 (MQ=255)                                                                                                                                                                                                   gCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCa                     <  2:351407/251‑1 (MQ=255)                                                                                                                                                                                                                tGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTc        >  1:147707/1‑251 (MQ=255)                                                                                                                                                                                                                      tCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCttg  >  2:90554/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       CCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTG  >  NC_000913/1974991‑1975454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑