Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,856,968	T→A	I268F (ATT→TTT)	ydjI ←	L‑glycero‑L‑galacto‑octuluronate‑1‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,856,968	0	T	A	100.0%	79.5 / NA	24	I268F (ATT→TTT)	ydjI	L‑glycero‑L‑galacto‑octuluronate‑1‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/10);  total (14/10)

ATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCCACATCAAAGATATTTTTACTGACCGGTTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGATCCCCGATGCACCGTGCAAAACCAGCGGAACAGAAACGGCATCGCGTACGCGTTTGACGACCTCAAAGTTCAA  >  NC_000913/1856728‑1857211                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    aTCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCCACATCAAAGATATTTTTACTGACAGGTTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAAt                                                                                                                                                                                                                                           >  1:286948/1‑251 (MQ=255)   cTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCCACATCAAAGATATTTTTACTGACCGGTTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGc                                                                                                                                                                                                                                         >  2:188839/1‑251 (MQ=255)             aCGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCCACATCAAAGATATTTTTACTGACCGGTTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCTATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCtttt                                                                                                                                                                                                                               >  1:97460/1‑251 (MQ=255)                                     gCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCCACATCAAAGATATTTTTACTGACCGGTTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCt                                                                                                                                                                                                       >  2:331434/1‑251 (MQ=255)                                         aGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCCACATCAAAGATATTTTTACTGACCGGTTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAAt                                                                                                                                                                                                   >  1:324423/1‑251 (MQ=255)                                                                    aTTGCATCACCCCCGGTGGTCATTGCGATTCTTTCACGAGGATAAGAATCCACATCAAAGATATTTTTACTGACCGGTTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTCTCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTtttctt                                                                                                                                                                        >  1:334138/1‑251 (MQ=255)                                                                                   gtggtCATTGCGATTCTTTCAAGAGGATAAGAATCCACATCAAAGATATTTTTACTGACCGGTTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTg                                                                                                                                                         <  2:208858/251‑1 (MQ=255)                                                                                            gCGATTCTTTCAAGAGGATAAGAATCCACATCAAAGATATTTTTACTGACCGGTTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGAc                                                                                                                                                <  1:74451/251‑1 (MQ=255)                                                                                                ttCTTTCAAGAGGATAAGAATCCACATCAAAGATATTTTTACTGACCGGTTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACag                                                                                                                                              >  1:276141/1‑249 (MQ=255)                                                                                                                 gAATCCACATCAAAGATATTTTTACTGACCGGTTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTg                                                                                                                            >  2:109439/1‑250 (MQ=255)                                                                                                                           cAAAGATATTTTTACTGACCGGTTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGAt                                                                                                                 >  2:310339/1‑251 (MQ=255)                                                                                                                              aGATATTTTTACTGACCGGCTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTGAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCAGTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATAcc                                                                                                              <  2:59303/251‑1 (MQ=255)                                                                                                                                               ggTTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGttt                                                                                             <  1:336186/251‑1 (MQ=255)                                                                                                                                                   gCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTTCAGTTTTAAt                                                                                         <  2:138150/251‑1 (MQ=255)                                                                                                                                                                  caaTAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGAt                                                                          <  1:331434/251‑1 (MQ=255)                                                                                                                                                                  caaTAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGAt                                                                          >  2:287694/1‑251 (MQ=255)                                                                                                                                                                          gCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTACACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGATCCCCGATg                                                                  >  1:326869/1‑251 (MQ=255)                                                                                                                                                                                                       cATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGATCCCCGATGCACCGTGCAAAACCAGCGGAACAGAAACg                                     >  2:169398/1‑251 (MQ=255)                                                                                                                                                                                                          atCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGATCCCCGATGCACCGTGCAAAACCAGCGGAACAGAAACGGc                                   >  2:26608/1‑250 (MQ=255)                                                                                                                                                                                                                         tgacATCAGAACCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGATCCCCGATGCACCGTGCAAAACCAGCGGAACAGAAACGGCATCGCGGACGCGttt                    <  1:63970/247‑1 (MQ=255)                                                                                                                                                                                                                                  aaCCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGATCTCCGTATGAATGTTGATTTTCGCGATAc                                                                                                               >  2:83558/1‑150 (MQ=255)                                                                                                                                                                                                                                  aaCCGAACAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATAc                                                                                                               <  1:83558/150‑1 (MQ=255)                                                                                                                                                                                                                                        aCAGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGATCCCCGATGCACCGTGCAAAACCAGCGGAACAGAAACGGCATCGCGTACGCGTTTGACGACCTCAAAGTTc    <  1:251838/251‑1 (MQ=255)                                                                                                                                                                                                                                          aGTTTAAATTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGATCCCCGATGCACCGTGCAAAACCAGCGGAACAGAAACGGCATCGCGTACGCGTTTGACGACCTCAAAGTTCaa  <  1:169398/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    ATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCCACATCAAAGATATTTTTACTGACCGGTTGCAATGGAATATCAACAATAGCGGCACCTATACAAATAACGTCGAGATTATCCATATCATTATTCCGCTTTGCCATCAGAACCGAACAGTTTAATTTTTTCCAATGCCCGTTCTTTTACAGCTTTACGTACTTCACGTTCTAAATGCAGGAAAGGCTGATCCTGGTTTTCTTTCACTGCAACCATTGCGGCCTGACAGAGCTCCGTATGAATGTTGATTTTCGCGATACCCAACGAAATTGCAGTTTTAATGTCGGCATCACTGATCCCCGATGCACCGTGCAAAACCAGCGGAACAGAAACGGCATCGCGTACGCGTTTGACGACCTCAAAGTTCAA  >  NC_000913/1856728‑1857211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑