Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,736	T→G	S325A (TCC→GCC)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,736	0	T	G	96.0%	75.6 / ‑3.1	25	S325A (TCC→GCC)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (14/10);  total (15/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

GAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACA  >  NC_000913/1306496‑1306971                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            gAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTg                                                                                                                                                                                                                                   <  1:275327/251‑1 (MQ=255)              atgatgAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGa                                                                                                                                                                                                                       <  1:27341/250‑1 (MQ=255)                          aCCACAAGCCACTACATCCTTACACCAGGGCATTGATGTCTGCAGTCCCCATACCTGATCCGGATCTGGAGAAGACCAAAACCATCCAGTTACTGGAAGGGGAATTACCGACCCCGATCACCCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCATACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTaa                                                                                                                                                                                                             <  2:173881/248‑1 (MQ=255)                                              tACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGt                                                                                                                                                                                      >  1:274140/1‑251 (MQ=255)                                                  ccAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGc                                                                                                                                                                                  <  2:274140/251‑1 (MQ=255)                                                    aGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGCCACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGccc                                                                                                                                                                                <  2:51389/251‑1 (MQ=255)                                                       gCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCttt                                                                                                                                                                             >  2:153000/1‑251 (MQ=255)                                                         aTTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGCGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCttttt                                                                                                                                                                           >  2:331185/1‑251 (MQ=255)                                                          ttGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAg                                                                                                                                                                                                   >  2:328578/1‑226 (MQ=255)                                                          ttGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAg                                                                                                                                                                                                   <  1:328578/226‑1 (MQ=255)                                                             aTGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTCCTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGCGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACg                                                                                                                                                                       >  1:128865/1‑251 (MQ=255)                                                                                    gccGGATCTGGGGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTCCCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCGGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCg                                                                                                                                                <  2:124193/250‑1 (MQ=255)                                                                                                            cATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTtgtg                                                                                                                        >  1:259498/1‑251 (MQ=255)                                                                                                                  gTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCttt                                                                                                                  >  1:179496/1‑251 (MQ=255)                                                                                                                                  ttACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCACAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAAc                                                                                                     >  2:178475/1‑248 (MQ=255)                                                                                                                                     ccGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATg                                                                                               >  1:213404/1‑251 (MQ=255)                                                                                                                                       gTCGCCGAACAACCCACCATCCCGTTGTGTTCTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGAGCCAAAACCCGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCg                                                                                             >  2:35620/1‑251 (MQ=255)                                                                                                                                           ccGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCCTAACAGAATGCGGGc                                                                                          >  2:170560/1‑250 (MQ=255)                                                                                                                                                      gCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGtt                                                                              >  1:117146/1‑251 (MQ=255)                                                                                                                                                                          gTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGt                                                          >  1:186891/1‑251 (MQ=255)                                                                                                                                                                                            ccGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAAGcacca                                        <  1:75984/251‑1 (MQ=255)                                                                                                                                                                                                 ccAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTc                                    <  1:150528/250‑1 (MQ=255)                                                                                                                                                                                                   agtatGCGACAAAACACGTCCTGTGCTGGCAGGGAGTTTCAGACACGCCGGTGCTCGCCTGCAGGTCTATCCGCTTTAGAACCAATAAGGGGTAACACTTGTTAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGACGCTATGGCACAGTTTATGATGTTTTTCGTGGTGTAATAGAATCCGGGCAGCAACATTGTCGATTTCACCATGTTCACTGTCCGCCACACCAACCACCACtacga                                  >  2:306533/5‑246 (MQ=255)                                                                                                                                                                                                                      ccTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCaa               >  2:210139/1‑250 (MQ=255)                                                                                                                                                                                                                                  gggATTTTCAGACCCGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATTCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCTCTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACa  <  1:328697/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            GAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACA  >  NC_000913/1306496‑1306971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑