Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,123,556	C→T	E167K (GAA→AAA)	grxB ←	reduced glutaredoxin 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,123,556	0	C	T	100.0%	89.1 / NA	25	E167K (GAA→AAA)	grxB	reduced glutaredoxin 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/10);  total (15/10)

ACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGC  >  NC_000913/1123329‑1123785                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      aCCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCCTATTATCGCGGGAATCAGCAACGCGGCTTGGCCAGGTAATTCCGGCCACCAGCGGCAGATTACGCAGGAGCGGGAATAACTGGATATCATCTTCCGAAAGTTTGCCATTCACCGCGTTTGGtttt                                                                                                                                                                                                                 >  1:65282/1‑249 (MQ=255)                                              cccGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTaa                                                                                                                                                                   <  1:257539/251‑1 (MQ=255)                                                     aTACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGAcc                                                                                                                                                            <  2:235913/251‑1 (MQ=255)                                                       aCAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCg                                                                                                                                                           <  1:295687/250‑1 (MQ=255)                                                        cAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTc                                                                                                                                                         <  1:168437/251‑1 (MQ=255)                                                           gCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCaga                                                                                                                                                      <  2:262640/251‑1 (MQ=255)                                                                   ggcggGTCAGGATTAAATCGCCATTGATTATAACAAATTGATTTGTGTCTGTTTCGCCATATTAGCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCGGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCca                                                                                                                                              <  2:334864/251‑1 (MQ=255)                                                                   ggcggGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCca                                                                                                                                              <  2:316849/251‑1 (MQ=255)                                                                                tAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCaa                                                                                                                                  >  1:126753/1‑250 (MQ=255)                                                                                                           aTTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTc                                                                                                      >  1:61888/1‑251 (MQ=255)                                                                                                            tttGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTAAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCACAATTACCCGCGCTGGCCTCTTTCTTGTcg                                                                                                     >  1:213633/1‑251 (MQ=255)                                                                                                                 gtCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGaa                                                                                                <  2:61888/251‑1 (MQ=255)                                                                                                                  tCTGATTCGCCATATGATCGCGGTAATCAGCACCGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGAGTACGCAGTAGCGGGCATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGCCGATCAGCTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGGGTGGGCCAGCCGGGAGGCAAAATTACCCGCGCTGGCAtctttctttccgtagaa                                                                                                >  2:344486/1‑241 (MQ=255)                                                                                                                                              aGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACt                                                                   <  1:4384/251‑1 (MQ=255)                                                                                                                                                 aaCGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACtcat                                                                >  1:262945/1‑251 (MQ=255)                                                                                                                                                    gcgGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTc                                                                                                                                                                                                                       <  2:149875/97‑1 (MQ=255)                                                                                                                                                    gcgGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTc                                                                                                                                                                                                                       >  1:149875/1‑97 (MQ=255)                                                                                                                                                      ggCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCaaa                                                            >  1:216008/1‑250 (MQ=255)                                                                                                                                                      ggCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGCAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGGGTAGAAAACTCATCaaa                                                            >  2:249432/1‑250 (MQ=255)                                                                                                                                                            gCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGAt                                                     >  1:335541/1‑251 (MQ=255)                                                                                                                                                                         ggCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCCCGGCGTTCGGGTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGTGTAGAAAACTCATCACATGCCGAATTTGCAAAAcgcg                                        >  2:243216/1‑251 (MQ=255)                                                                                                                                                                                      aGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGttt                           >  1:296356/1‑251 (MQ=255)                                                                                                                                                                                                     gggAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCAtt            >  1:82097/1‑251 (MQ=255)                                                                                                                                                                                                            gCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTg     >  2:110612/1‑251 (MQ=255)                                                                                                                                                                                                               gAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTgcgc  >  2:349960/1‑251 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      ACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGC  >  NC_000913/1123329‑1123785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑