Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,100,070	G→A	C144Y (TGT→TAT)	ycdY →	chaperone protein YcdY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,100,070	0	G	A	100.0%	113.1 / NA	32	C144Y (TGT→TAT)	ycdY	chaperone protein YcdY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/13);  total (19/13)

CCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGG  >  NC_000913/1099824‑1100294                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                 ccGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCtggtatggt                                                                                                                                                                                                                              >  1:146124/1‑251 (MQ=255)  cGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCtggtatggtg                                                                                                                                                                                                                             >  2:95903/1‑251 (MQ=255)   gATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGAGCGACGGAAGCGGAAAAGCGCGCTTTTCTTTCTGACCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGACACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGc                                                                                                                                                                                                                            >  1:169666/1‑251 (MQ=255)        cAATGCGTGGTTTATCGGCGATGAATGTGCTGTGCCGCCATACCGTAGCGCATGGGTTGAGGGCGCGCCGGAAGGGGCAGGGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGTCAGCCGATAACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGCAGATCAGTCAACGGAAGATGAGGGCGAAGCACTGGAAACACGGTTCAGTGGGTCTATGTGCCCCTGGTATGGTGCGTTcc                                                                                                                                                                                                                       >  2:127951/1‑251 (MQ=255)              gttttttACCGGGGATGAGTGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGAGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGAGGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCGGGTATGGTGCGTTCCTTGGCa                                                                                                                                                                                                                 <  2:47729/247‑1 (MQ=255)               ttgttctacggcgccgAATGTGCCGTGCCCGCATACCATAGCGCATGGGTTGAGAGCGCGACGGAAGCGGCAGTGCGCGCTTTTCTTTCTGAGAGAGGGATGACATTAGCGGATACGCCAGCCGATCACATCGGCACCTTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGCAGCACTGGAAAAACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCa                                                                                                                                                                                                                 >  2:144965/16‑250 (MQ=255)                                              cATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTgg                                                                                                                                                                                 >  1:20302/1‑251 (MQ=255)                                                               gTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCt                                                                                                                                                                <  2:139624/251‑1 (MQ=255)                                                                  gAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAAc                                                                                                                                                             >  2:309397/1‑251 (MQ=255)                                                                   aGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAAcc                                                                                                                                                            >  1:239025/1‑251 (MQ=255)                                                                       cgcgACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCcgcg                                                                                                                                                        >  1:322251/1‑251 (MQ=255)                                                                         cgACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGCGCGAAGCACTGGAAACACTGTTCCGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGAt                                                                                                                                                      >  2:309806/1‑251 (MQ=255)                                                                              gaagcggaagTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCAGATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCAt                                                                                                                                                 <  2:196474/251‑1 (MQ=255)                                                                              gaagcggaagTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCCACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCAt                                                                                                                                                 >  2:273054/1‑251 (MQ=255)                                                                                ggcggaagTGCGCGCTTTTCTTTCTGAGGGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTa                                                                                                                                               <  2:75029/250‑1 (MQ=255)                                                                                     aagTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGc                                                                                                                                           >  2:124607/1‑250 (MQ=255)                                                                                              cTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGAc                                                                                                                                 >  1:91360/1‑251 (MQ=255)                                                                                                             gAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGgaaga                                                                                                                      >  1:61541/1‑247 (MQ=255)                                                                                                              aGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAtt                                                                                                                 >  1:320449/1‑251 (MQ=255)                                                                                                                             ggATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAgtgt                                                                                                  >  2:101991/1‑251 (MQ=255)                                                                                                                                 aCGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTTGCTGGAAGAGCAGTCAACGTAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGag                                                                                               <  2:221911/250‑2 (MQ=255)                                                                                                                                 aCGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATc                                                                                              >  1:190054/1‑251 (MQ=255)                                                                                                                                       gCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTc                                                                                        <  1:23406/251‑1 (MQ=255)                                                                                                                                          gATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGATTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTTTGATCTACGTCACt                                                                                     <  1:315444/251‑1 (MQ=255)                                                                                                                                               caTCGGCACATTGCTGCTCGCAGCTGACTGGCGGGAAGAGCAGTCAACGGAAGATGAGAGGGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCTCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCagta                                                                                <  2:277502/251‑4 (MQ=255)                                                                                                                                                           gctgctCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGgc                                                                                                                                                                                <  2:343367/143‑1 (MQ=255)                                                                                                                                                           gctgctCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGgc                                                                                                                                                                                >  1:343367/1‑143 (MQ=255)                                                                                                                                                           gctgctCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGAt                                                                     <  1:201493/250‑1 (MQ=255)                                                                                                                                                                            ggctggAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATACCTGCAACGGATAATTTGTTGTTGCATaa                                                    >  2:284155/1‑250 (MQ=255)                                                                                                                                                                                ggAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAAt                                                 <  2:61541/249‑1 (MQ=255)                                                                                                                                                                                 gAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAAtgtg                                              <  2:20302/251‑1 (MQ=255)                                                                                                                                                                                    gATCAGTCAACCGCAGATGCGAGCGAAGCACTGGAAACCATGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGc                                           >  2:324933/1‑251 (MQ=255)                                                                                                                                                                                                                        acacTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATg       <  1:309397/251‑1 (MQ=255)                                                                                                                                                                                                                             gTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCgg  >  2:225562/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                 CCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGG  >  NC_000913/1099824‑1100294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑