Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,061,9350GA93.8% 80.7 / ‑6.1 32S46N (AGC→AAC) torDtrimethylamine‑N‑oxide reductase‑specific chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/16);  minor base C (0/2);  total (14/18)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAAC‑GGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTC‑TCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGC  >  NC_000913/1061701‑1062178
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   
gagaAGTACAACGGAACAGTGGAGCAGGAGACGGCGTTTAAC‑GGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTC‑TCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACt                                                                                                                                                                                                                                     >  2:89276/1‑251 (MQ=255)
         aaCGGAACAGTGGAGCAGGTGACGGCGTTTAAC‑GGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTC‑TCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTg                                                                                                                                                                                                                            >  1:358309/1‑251 (MQ=255)
               aCAGTGGAGCAGGTGACGGCTTTTACC‑GGCCCCGTGGAGAGGGTGGCGCAGTGCGAATATTTTCCCGCGTCGAAGGTAAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTTTTTCCGCCGGGCTAGCGCAGTTGTTC‑CCCCGTGAGCTGGACGATGAACAACTGACGCAAATCCCCAGTGCGCAGATGGCTGAATGGTTTTGGTTGCTA‑AAAAACGATCCGCCGCTCACTGCGGCGGTGAACGAg                                                                                                                                                                                                                      <  1:260968/251‑1 (MQ=255)
                      aGCAGGTGACGGCGTTTAAC‑GGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTC‑TCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGCATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAAAGAGCTGGaa                                                                                                                                                                                                                >  2:47690/1‑250 (MQ=255)
                       gcgggggACGGC‑TTTAACCGGCCCGGTGGGGATGGGGGGCCAGTGGAAATATTTCCCCGCGTCGCGGGTGAAACCAGGCCCCCGCTGACAGCCAAACAAATGGCTTGTGTTTACGCCTGGCTGGCGCAGTTGTTC‑CCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGAGGGCTGAATGGTTTGCGTTGCTA‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGaaaa                                                                                                                                                                                                              <  2:356160/245‑1 (MQ=255)
                            tGACGGCGTTTAAC‑GGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTC‑TCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGt                                                                                                                                                                                                          >  1:232850/1‑250 (MQ=255)
                             gACGGCGTTTAAC‑GGCCCCGTGGAGATGGTGGCGCGGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACACCAGATTGCCTGTGTTTACGCCTGGCTAGCGCATTTGTTC‑CCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTAt                                                                                                                                                                                                        <  1:110083/251‑1 (MQ=255)
                                 gCGTTTAAC‑GGCCCCGTGGAGATGGTGGCGCAGTGCGATTATTTCCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTC‑TCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGcc                                                                                                                                                                                                    <  1:22203/251‑1 (MQ=255)
                                      tAAC‑GGCCCCGTGGAGATGATGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCAAGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTC‑TCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGGGATGGAAAACCGTATTGCCACGcc                                                                                                                                                                                               >  2:200722/1‑250 (MQ=255)
                                                cGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTC‑TCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAATCGTATTGCCCCGATGACAGTACg                                                                                                                                                                                      >  1:297599/1‑251 (MQ=255)
                                                                        ataggTTCCGGCGTCGCAGGTGAAAGCAGGACCACGCGGACAGCACAACAGCTTGCTTGGGTTTACGCCTGGCAGGCGCAGTGGTGC‑GCCCTGGGGCGGGAAGATAACCAACTGACGAAAATCGCCAGTGCGAGGAGGGGTGAGTGGTTTTCGTTGCGG‑AAAACCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGc                                                                                                                                                              <  2:97470/247‑1 (MQ=255)
                                                                           tGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTC‑TCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGGATTGCCACGCTGACCGTACGTGACGATGCCCGTCTGGAACCGGCcgc                                                                                                                                                           >  2:79456/1‑251 (MQ=255)
                                                                                   cgTCGCAGGGAAAATCTTGCCCACGCTGACGGCACACAAGATGCCCTGTTTTTACGCCTGGCTGTCGCTGT‑GTTCCTCCCTGGAGCGGGACGAGGAACATCTGACGCAAATCGCCAGGGCGCAGAGGTCTGAATGGTTTTCGTTGCTG‑AAAAACTAACCGCCGCTCACTGCGGCGGTGAACGAGCTGTAAAACCGTATTGCCACGCTGACATTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACtttt                                                                                                                                                   <  1:56592/251‑1 (MQ=255)
                                                                                                         aCGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTC‑TCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCg                                                                                                                              >  2:152210/1‑250 (MQ=255)
                                                                                                                                           ccTGGCTAGCGCAGTTGTTC‑TCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTAc                                                                                           >  2:65208/1‑251 (MQ=255)
                                                                                                                                                  aGCGCATTTGTTC‑TCCCGGGAGCTGGACGATGAACAACTGACGAAAATCGCAAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACa                                                                                      <  1:51197/249‑1 (MQ=255)
                                                                                                                                                   gcgcATTTGTTC‑TCCCTGGAGCTGTGCGATGAACATCGGACGCAAATGGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTA‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGTTCTGTTTCTGATTACCGTCAATCAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGa                                                                                   <  1:40077/251‑1 (MQ=255)
                                                                                                                                                    cgcAGTTGTTC‑CCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTA‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGAc                                                                                  <  1:65208/251‑1 (MQ=255)
                                                                                                                                                        ggttgttcccccgtgagtgggtgtaTGAACAACTGACCCAAACCGCCGGTGCGCAGTTGGCTGAATGTTTTTCGTTGCAG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACGGGCCGCGGACTTTTGCGGCCTGTTGCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAg                                                                               <  2:152042/227‑1 (MQ=255)
                                                                                                                                                                     ggAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAAc                                                                  <  1:21290/250‑1 (MQ=255)
                                                                                                                                                                        gCTGGACGATGAACAACTGACGAAAACCGCCGGTGCGCAGAGGGCTGAATGGTTTTCTTGGCGG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCt                                                               <  1:106641/251‑1 (MQ=255)
                                                                                                                                                                                  gAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTg                                                      >  1:329720/1‑250 (MQ=255)
                                                                                                                                                                                            cGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAACACGAACCGCCGCTCACTGCGGAGGTGAACGAGCTGGAAAACCGTATTGCCACGCTAGCAGTACGTGACGATGCCCGTCTGGTACTGGCCGCGGACTTTTGCGGCCTGTTTCTGCTGAACGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACCAGCCAGAGATTAAACGCTTGTTAGTTTAGGCAGGGa                                             >  1:183961/1‑249 (MQ=255)
                                                                                                                                                                                             gCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATgg                                          >  1:173448/1‑251 (MQ=255)
                                                                                                                                                                                                aaccGCCAGGGCGCAGTGGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGTTTAAACGCTTGTTAGTTGAGGCAGGGATGGaaa                                       <  1:325982/248‑1 (MQ=255)
                                                                                                                                                                                                   cGCCAGTGCGAAGAGGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCa                                    <  1:217445/251‑1 (MQ=255)
                                                                                                                                                                                                     ccAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGGAAACCGTATTGCCACGCTGACAGTACGGGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCCGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCCACAAACAGGACGAGCAAGAGCTTAAACCCTTGTTAGGTGAGGCAGGGATGGGAAcccac                                  >  2:267280/1‑248 (MQ=255)
                                                                                                                                                                                                         ggcgcagtgggCTGAAGGGTTTTCGTTGCTG‑AAAACCGATCCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCa                              <  2:81104/242‑1 (MQ=255)
                                                                                                                                                                                                                 gggCTAAATGGTTTTC‑TTTCTGGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCaa                       <  1:200722/249‑1 (MQ=255)
                                                                                                                                                                                                                                 gTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGc                                                                                                            <  2:118792/149‑1 (MQ=255)
                                                                                                                                                                                                                                 gTTGCTG‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGc                                                                                                            >  1:118792/1‑149 (MQ=255)
                                                                                                                                                                                                                                       g‑AAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGc  <  1:152210/249‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   
GAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAAC‑GGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTC‑TCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTG‑AAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGC  >  NC_000913/1061701‑1062178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑