Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	968,837	G→A	D158N (GAT→AAT)	lpxK →	tetraacyldisaccharide 4'‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	968,837	0	G	A	100.0%	101.9 / NA	30	D158N (GAT→AAT)	lpxK	tetraacyldisaccharide 4'‑kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/16);  total (14/16)

GGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGT‑CGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCC‑GGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGC  >  NC_000913/968604‑969072                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               gggTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGtt                                                                                                                                                                                                                               <  1:321085/251‑1 (MQ=255)          cgcgGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATgg                                                                                                                                                                                                                       <  2:279780/250‑1 (MQ=255)          cgcgGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGGTGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTTTCCCGTTCGTTCTGATGCGGTAAAAGCCCTTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGGTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGgt                                                                                                                                                                                                                      >  1:120736/1‑251 (MQ=255)              gggATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGGAACTGTGTTGATTTATCAAAGCACTGATGCGCCCGTTGCGGGTTTTCCCGTTCGTTCTGATGGGGGAAAAGCCATTTTGGGGCAACACCCTGATGTGCAGATCATCGTAAACGACGAACGGTTTA‑AGCATTACCGTCTGGGGCGTAATGTGGAAATTTTCGTTATTGATGgtgggg                                                                                                                                                                                                                  >  2:69349/1‑248 (MQ=255)               ggATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGCTGCGGTAAAAGCCATTCTGGCGCAACACCCTGATTTGCAGATCATCGTAACCGACGA‑CGGGTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTTTCGTTATTGATGGTGTGCg                                                                                                                                                                                                                 >  1:286428/1‑251 (MQ=255)                 atatGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTc                                                                                                                                                                                                               <  2:66231/251‑1 (MQ=255)                          tAAGGCTGAATCTTATCCGCTGTTATTGTCGGCCGATACCACAACCGCACAGGCGGGTGCTGAACCTCAGTTGATTTATCACAAAACTCCTGACCCCCTTGCGGCTTATACCGGTCCTTCTTATGAGGAAAAAGCCCCTTTAGCGCAAAAACCCGATGTGCAGATCATCGTAACCCACGA‑CGGATTACAGAATTAACGTCTGGAGGGTAATGTGGAAATTGTCGGTTTTGATGGTGTGCGTCGCttttga                                                                                                                                                                                                       >  1:18176/1‑247 (MQ=255)                           aaGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAAACTGTGTTGATTTATTAACGCACTGATGCGCCTGTTGCGGGTTCTCCCGTTCGGTCTGATGCGGTAAAAGCCCTTCTGGCGCAACACCCTGATGTGCAGAGCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGGGTGGGTCGGTTTggga                                                                                                                                                                                                      >  1:241515/1‑248 (MQ=255)                                       tATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGATGAACCTGTGTTGATTTTTCAACGCACTGATGCGCCTGTTGCGGGTTTTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCCTTGGCAATGGCTGGGGGtt                                                                                                                                                                                         >  1:156747/1‑251 (MQ=255)                                                       cGGCAGATACCACAACAGCACAGGCGGGTGGTGAACCTGTGTTGATTTTTCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCCTTCGTTCTGATGCGGGAAAAGCCCTTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGGAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGGTGCCGgggggggccatg                                                                                                                                                                         >  1:132881/1‑245 (MQ=255)                                                               aCCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAAtt                                                                                                                                                                                                                                     <  1:350400/183‑1 (MQ=255)                                                                    aacaGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCgg                                                                                                                                                           <  1:296520/251‑1 (MQ=255)                                                                                             gtgtTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGT‑CGGTTGATGCGGt                                                                                                                                 <  2:262745/251‑1 (MQ=255)                                                                                                  gATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGT‑CGGTTGATGCGGTAATCg                                                                                                                            <  2:156747/251‑1 (MQ=255)                                                                                                      tATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTACAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGGCCAAT‑CGGGGGCGCGGGGGGCGCTTAAAGTGCGGT‑GATGCCGTAATCggca                                                                                                                         >  2:46507/1‑247 (MQ=255)                                                                                                      tATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGT‑CGGTTGATGCGGTAATCGTCa                                                                                                                         <  1:47474/250‑1 (MQ=255)                                                                                                       aTCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGTCGCGTAATGTGGAAATTGTCGTTATTGATGGTGGGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGT‑CGGTTGATGCGGTAATCGTCaa                                                                                                                        <  1:225821/250‑1 (MQ=255)                                                                                                              cacggATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACCCCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGT‑CGGTTGATGCGGTAATCGTCAACGGCGgtg                                                                                                                <  2:263284/247‑1 (MQ=255)                                                                                                                  gATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGT‑CGGTTGATGCGGTAATCGTCAACGGCGGTGTc                                                                                                              <  2:63687/249‑1 (MQ=255)                                                                                                                    tGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGT‑CGGTTGATGCGGGAATCGTCCACGGCGGGGTccccc                                                                                                          >  1:179691/1‑249 (MQ=255)                                                                                                                      cgcCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCCATGCGTGAGCGAGCGGGGCGCTTTAAGT‑CGGGTGGTGCGGGAATCGTCCAcggcggggtggccccc                                                                                                        >  2:57210/1‑241 (MQ=255)                                                                                                                        ccTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGT‑CGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAg                                                                                                      <  1:339304/251‑1 (MQ=255)                                                                                                                                                            aaaGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGT‑CGGGTGATGCGGTAATCGTCAACGGCGGGGTCCCCCCCAGCGGGGGAAACCCCCTGCCTCTGCTGCC‑GGGTCAggg                                                                 >  1:328424/1‑250 (MQ=255)                                                                                                                                                                               acCCTGATGTGCAGATCATCGTACCCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑GCAATGCGTGAGCGAGGGGGGCGCTTAAAGT‑CGGTTGATGCGGGAATCGTCAACGGCGGGGTCCCTCGCAGCGGGGGAAACCCCCTGCATCTGCTGCC‑GGGGCAGGGGGGGGATTTTCGTACCggg                                              >  1:269937/1‑250 (MQ=255)                                                                                                                                                                                   tGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGGGCTTAAAGG‑CCGGTGATGCGGTAATTGTCAACGGCGGGGGGCCTCGCAGCGGGGAAATCCCCCTGCCTCTTCGGCCCGGGTCAgcggggggatttactacctgggtgc                                           >  2:189029/1‑227 (MQ=255)                                                                                                                                                                                         gCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGT‑CGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCC‑GGGTCAGGCGGTGAATTTACGTACCGGTACGCGTtgt                                     <  1:348491/250‑1 (MQ=255)                                                                                                                                                                                                               cgGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGTCTGGTGGTTGCCGGCGGGG‑CCAATGCGTgagcgagc                                                                                                                                                             <  2:353733/111‑1 (MQ=255)                                                                                                                                                                                                               cgGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTgagcgagc                                                                                                                                                             >  1:353733/1‑111 (MQ=255)                                                                                                                                                                                                                 gTTTACAGCATTACCTTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCGTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGT‑CGGTTGATGCGGTAATCGGCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCC‑GGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTa             <  2:23862/251‑1 (MQ=255)                                                                                                                                                                                                                             aCCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGT‑CGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCC‑GGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGc  <  2:286428/250‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               GGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGA‑CGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGT‑CGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCC‑GGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGC  >  NC_000913/968604‑969072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑