Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	791,688	G→A	S123F (TCC→TTC)	galE ←	UDP‑glucose 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	791,688	0	G	A	100.0%	142.2 / NA	40	S123F (TCC→TTC)	galE	UDP‑glucose 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/22);  total (18/22)

GATGTATGGCATCAGGTTATTCGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCG  >  NC_000913/791444‑791924                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             gATGTATGGCATCAGGTTATTCGGAATGACTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTaa                                                                                                                                                                                                                                        <  1:214659/251‑1 (MQ=255)     tATGGCATCAGGTTATTCGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAAt                                                                                                                                                                                                                                    >  1:114006/1‑251 (MQ=255)             cAGGTTATTCGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGtttt                                                                                                                                                                                                                            <  2:39651/251‑1 (MQ=255)             cAGGTTATTCGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGtttt                                                                                                                                                                                                                            <  1:119486/251‑1 (MQ=255)                gTTATTCGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGa                                                                                                                                                                                                                         <  1:215869/251‑1 (MQ=255)                      cGGAATGCCTTGCGGCTCTTCGCCCATATCGCCCGACGGATCCGCGCCAACCGGGTTGACGAAGCGCAGCAGGGCAATGCTCCCGTCCGGCTGGGCTTTTTGCAGCTCGGTGAGGATCTGTTCCCCCATCAGCTTGCTTTTGCCCTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGtt                                                                                                                                                                                                                     >  1:28318/1‑249 (MQ=255)                      cGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTa                                                                                                                                                                                                                    <  2:334668/250‑1 (MQ=255)                      cGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAg                                                                                                                                                                                                                   <  1:102374/251‑1 (MQ=255)                         aaTGCCTTGCGGATCTTCGCCCATATCGCCCGAACGATCCCCGCACACCGCGATGACGTAGCGCACCACGCCAATGCTACAGTCAGCCAGGGCTCTTTGCAGATAGGTGCGGATCTGTTCCACCATCAGCTTGCTTATGCCGTACGGGCTTTGCGGCGTGCCGGTCGGGAAGCATTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGCGGCGGAGAAGCTACAAATAAAGTTTTTGACGTTAgcgg                                                                                                                                                                                                                >  1:163461/1‑251 (MQ=255)                                 gCGGATCTTCGCCCATATCGCCCGACGGATGCGCCACAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATg                                                                                                                                                                                                        <  1:338806/251‑1 (MQ=255)                                         tCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAAt                                                                                                                                                                                                >  1:9026/1‑251 (MQ=255)                                         tCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGGTTTTGACGTTAGCGGCGCGCATGgcg                                                                                                                                                                                                     <  2:285891/246‑1 (MQ=255)                                           gCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCa                                                                                                                                                                                              <  2:230951/251‑1 (MQ=255)                                               atatCGCACGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGgcg                                                                                                                                                                                          <  2:105745/251‑1 (MQ=255)                                                 atCGTCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCATTGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCATCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCTCGCATGGCGCTAATCAGGCGCa                                                                                                                                                                                        <  1:281417/251‑1 (MQ=255)                                                                        ccGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCg                                                                                                                                                                 <  1:126864/251‑1 (MQ=255)                                                                          gggTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTa                                                                                                                                                               <  1:154963/251‑1 (MQ=255)                                                                            gTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAat                                                                                                                                                             >  1:56303/1‑251 (MQ=255)                                                                                aaGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTc                                                                                                                                                         <  2:10824/251‑1 (MQ=255)                                                                                                cAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTAcc                                                                                                                                         <  2:284224/251‑1 (MQ=255)                                                                                                         aGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCTGTACGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTaaa                                                                                                                                                                                                                                       >  1:39909/1‑148 (MQ=255)                                                                                                         aGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCTGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAAGTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTaaa                                                                                                                                                                                                                                       <  2:39909/148‑1 (MQ=255)                                                                                                            ccGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACg                                                                                                                             >  1:219547/1‑251 (MQ=255)                                                                                                                    gCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTc                                                                                                                       >  1:251155/1‑249 (MQ=255)                                                                                                                     cTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGc                                                                                                                    >  1:235166/1‑251 (MQ=255)                                                                                                                                 cggtgtgactccgttagaccgtCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTACTCAGGCGCAGAGTGCAGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTgg                                                                                                        >  2:208741/22‑251 (MQ=255)                                                                                                                                  ggTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGAGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGGACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTgg                                                                                                        >  2:152879/1‑250 (MQ=255)                                                                                                                                       ggATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACg                                                                                                  <  1:98929/251‑1 (MQ=255)                                                                                                                                                     aTCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGCAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTg                                                                                    <  2:179280/251‑1 (MQ=255)                                                                                                                                                         gcttgcttTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCg                                                                                >  1:194891/1‑251 (MQ=255)                                                                                                                                                                   gCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGAtct                                                                      >  2:38252/1‑251 (MQ=255)                                                                                                                                                                     cGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCg                                                                    >  1:244192/1‑251 (MQ=255)                                                                                                                                                                      gTAAGGGCTTTGCGGTGTGCCGGTCGGGCAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGAAGAAGCTACAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCgg                                                                   >  2:175931/1‑251 (MQ=255)                                                                                                                                                                             cTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGtcatca                                                             >  1:139651/1‑250 (MQ=255)                                                                                                                                                                                        gCCGGTCGGGAAGCTTTCAACGGATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGAGCTCGGTCATCAACGCTTCGTTAc                                                 <  2:362074/251‑1 (MQ=255)                                                                                                                                                                                          cGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACg                                                >  2:294292/1‑250 (MQ=255)                                                                                                                                                                                                                 ggAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTg                         <  2:321176/250‑1 (MQ=255)                                                                                                                                                                                                                        tGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGttt                  <  1:235177/250‑1 (MQ=255)                                                                                                                                                                                                                                  gCCATAAACGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAAc       <  1:115585/251‑1 (MQ=255)                                                                                                                                                                                                                                       aaaCGGTGGCGGAGAAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGAAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCg  >  2:277324/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             GATGTATGGCATCAGGTTATTCGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCG  >  NC_000913/791444‑791924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑