Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	553,161	G→A	intergenic (‑61/+57)	purE ← / ← lpxH	N(5)‑carboxyaminoimidazole ribonucleotide mutase/UDP‑2,3‑diacylglucosamine diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	553,161	0	G	A	100.0%	87.5 / NA	25	intergenic (‑61/+57)	purE/lpxH	N(5)‑carboxyaminoimidazole ribonucleotide mutase/UDP‑2,3‑diacylglucosamine diphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/13);  total (12/13)

AACCGTTCTCTTCGGCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCAC  >  NC_000913/552914‑553406                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      aaCCGTTCTCTTCGGCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGaaaa                                                                                                                                                                                                                                                    <  2:80424/251‑1 (MQ=255)     gTTCTCTTCGGCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAag                                                                                                                                                                                                                                                   >  2:292743/1‑248 (MQ=255)                      cGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGc                                                                                                                                                                                                                               <  1:178690/251‑1 (MQ=255)                        gCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGt                                                                                                                                                                                                                             >  1:283983/1‑251 (MQ=255)                                       ttATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGc                                                                                                                                                                                                              >  2:279589/1‑251 (MQ=255)                                                     gTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGttt                                                                                                                                                                                                >  1:250945/1‑251 (MQ=255)                                                                          gtggtgCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCa                                                                                                                                                                           <  1:47227/251‑1 (MQ=255)                                                                                        tCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATc                                                                                                                                                              <  1:214609/250‑1 (MQ=255)                                                                                                     gATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCa                                                                                                                                                <  1:122581/251‑1 (MQ=255)                                                                                                                  agcTGCATGGTAGCCCATTCGCTTTTGGCCCCCATCACGATGGCGACACGCGCCGGATTATTGGGGGAAGACA‑TGCGTCTAAAAACCCCTGTGGTGCACAACTCTCGGCTTGAGAGGGCAAAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGACTCAGCTCACCTTCATCCGCCGTGACTTTCACCATTGAACCTTc                                                                                                                                   <  2:255087/249‑1 (MQ=255)                                                                                                                     tGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGt                                                                                                                                >  1:189025/1‑251 (MQ=255)                                                                                                                       cATGGTAGCCCAGTCGCTTTTGGACCCCAGCACGAGGGCGACACGCGCCGGATTATTGCTGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGATAGCAAGGAAAACGGTTGCGTGTCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATACGCCGTTACTTTCCCCATTGAATCTTCCggat                                                                                                                              <  2:298740/251‑4 (MQ=255)                                                                                                                        aTGGTTGCCCAGTCGCTTTTGGAACACATCACGATGGCGACACGCGCAGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGCGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGAGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCCTCCGCCGTGACTTTCACCATTGAACATTCCGTATg                                                                                                                             >  2:17705/1‑251 (MQ=255)                                                                                                                          ggTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGcc                                                                                                                           >  1:80015/1‑251 (MQ=255)                                                                                                                                                 ccATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGaaa                                                                                                     >  2:274711/1‑250 (MQ=255)                                                                                                                                                                       cGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATa                                                                               >  1:292223/1‑250 (MQ=255)                                                                                                                                                                            tattGCGGAAAGACAGTG‑GTCTAAAAACTCCTGGGGTGAAAAACTCTCGGCTTGAGAGGGCAAAGAGAAGAGCACGAAAAGAGAGAAAGGAAAAGGGTTGGGTGGCTGTGAAATCAGCAATGTTGCGGGTTTTTTAAAACGGAAAATGAACCAGCTCATCTTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCATGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTc                                                                          <  2:213258/250‑1 (MQ=255)                                                                                                                                                                                 ggggAAGACA‑TGCGTCTTAAAACTCCTGGGGTGCACAACTCTCGGCTGTAGAGGGCACAGATAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTTAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCAc                                                                    <  2:209579/250‑1 (MQ=255)                                                                                                                                                                                     aaGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAGCGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTAGAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCAccgcc                                                                <  2:250945/251‑1 (MQ=255)                                                                                                                                                                                          a‑TGCTTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGAACAGAGAATAGCACGAAAAGAGAGCAAGGAAACCGGTTGCGTGGTTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCg                                                           <  1:246668/251‑1 (MQ=255)                                                                                                                                                                                                tCTTAAAACCCCTGGGGTGCGCAACTCTCGGCTTTAGGGGGAACAGAGAATAGCAGGAAAAAAGAGCAAGGAAAACGGTTGGGGGTTTGTAAAATCAGAAAAGTTGCGGTTTTTTAAAAACGAAAAATGAATCAGCTAAACTGCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATg                                                        <  2:281637/249‑1 (MQ=255)                                                                                                                                                                                                                            cGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTg                          >  2:5166/1‑251 (MQ=255)                                                                                                                                                                                                                            cGGCTTTAGAGGACACAGAGAATAGCACGAAAAGAGAGAAAGGAAAACGGTTGCGGGGCTGTGAAATCAGAAAAGTTGCGGGTTTTTAAAAACGGAAAATGAACCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCt                           <  2:189025/250‑1 (MQ=255)                                                                                                                                                                                                                                            agagAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGc           >  2:274811/1‑250 (MQ=255)                                                                                                                                                                                                                                                  tAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGacc    >  1:169369/1‑251 (MQ=255)                                                                                                                                                                                                                                                    gCACGAAAAGAGGGCAAGGAAAACGTTGGCGGGGCTGTAAAATCAGCAAAGTTGCGGGTTTTTTAAAAGGGAAAAGGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCAGGCACCGCCGGGCTATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGaccac  <  1:237818/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      AACCGTTCTCTTCGGCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACA‑TGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCAC  >  NC_000913/552914‑553406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑