Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	246,747	G→T	S12S (TCG→TCT)	yafL →	NlpC/P60 family protein YafL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	246,747	0	G	T	100.0%	247.4 / NA	68	S12S (TCG→TCT)	yafL	NlpC/P60 family protein YafL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/27);  total (41/27)

GTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCT  >  NC_000913/246507‑246984                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              gTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGt                                                                                                                                                                                                                                     <  1:254875/251‑1 (MQ=255)  tATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGtt                                                                                                                                                                                                                                    >  1:151455/1‑251 (MQ=255)  tATCTACTGTGCTGTATATTTCTTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGtt                                                                                                                                                                                                                                    >  2:50586/1‑251 (MQ=255)   atttcCTGGGCTGTAAATTTATTCAGCTTGAATTGGAGCTCAAATGAGCAACCACGGAAAACCAAGAGTAAATTAATAAACGGATGTTTTTAAATAATTAAACGAAATATAAAAGTTAAATTTTTAATAAAGGGTTATTAAGGTTGTTCCGAAACGATCCGGGATACACTGCAAAATTAAAGCATGACCCGGCCTTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTg                                                                                                                                                                                                                                   <  1:280925/248‑1 (MQ=255)       ccTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTg                                                                                                                                                                                                                                   <  2:259184/247‑1 (MQ=255)       ccTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGAttt                                                                                                                                                                                                                               >  1:255302/1‑251 (MQ=255)            gcggTATATTTATTCAGCTTGAATTGTAGCTCAAATGATCAACAGCGACAATCCATTAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGCCCAGCCATTATTGAGTATTTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTtgttttgttt                                                                                                                                                                                                                           <  2:284703/247‑1 (MQ=255)               gTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCg                                                                                                                                                                                                                       <  1:199000/251‑1 (MQ=255)                tataTTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGt                                                                                                                                                                                                                      >  1:154579/1‑251 (MQ=255)                   atttattCGGCTTGAAGTGTAGCTCAAGTGAGCAACAGCGACTATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑ATGGATTACTGTTGATTTGTGTGCCGtttt                                                                                                                                                                                                                   >  2:8759/1‑251 (MQ=255)                                gAATTGTAGCTCAAATGAGCAACAGCGACAATCAATGAGTAATTTAATAAACGGATTTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAATTTTGTTCAGAAACGTTCCGGGATACACTGCAACATTAAAGCATGTCCAGCCAGTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCa                                                                                                                                                                                                       <  2:198125/250‑1 (MQ=255)                                                gAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACcacatcacat                                                                                                                                                                                      >  1:345243/1‑251 (MQ=255)                                                 aGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACata                                                                                                                                                                                     <  1:57013/251‑1 (MQ=255)                                                     aCAGCGACAATCCATGAGTAATTTAATAAACGGATGTCTTTACATAATTAACTGACATCTATATGTTAATTTTATAATAATGGTTTATTAAGTCTGTTCAGAAACGATCCGGGATACACTGCAACATCAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATAtctc                                                                                                                                                                                 >  1:234420/1‑251 (MQ=255)                                                            cAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGCTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGc                                                                                                                                                                          >  1:353493/1‑251 (MQ=255)                                                                            taataaACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGc                                                                                                                                                          >  1:337003/1‑251 (MQ=255)                                                                                 aaCGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAAAAACGATCCGGGATCCACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCCCCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATg                                                                                                                                                     <  1:247547/251‑1 (MQ=255)                                                                                  aCGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGc                                                                                                                                                    >  1:14171/1‑251 (MQ=255)                                                                                  aCGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTAAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTACTTGCCGTCGATTCCATCATTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCTACCACATAAATTATCTCTTTCAGCTACGCCTCCCGCCAGCGGATGc                                                                                                                                                    >  2:120912/1‑251 (MQ=255)                                                                                                aTAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCGGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCCCATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGt                                                                                                                                      <  2:167115/251‑1 (MQ=255)                                                                                                 aaaTTAACTGAAATTTATATGTTAATTTTATAATAATGTTTTATTAGTTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATACCCATCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGtt                                                                                                                                     <  2:284085/250‑1 (MQ=255)                                                                                                      aaCTGAAATATATATGTTAATTTTATAATATTGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACCCTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCTCATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTttatt                                                                                                                                 <  1:312605/250‑1 (MQ=255)                                                                                                         tGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGCTGCAAAACCGTGCGCGTTTATTaaa                                                                                                                              >  2:13077/1‑250 (MQ=255)                                                                                                               atatatGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACTTTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCACAACCGTGCGCGTTTATTAAAACAGtcc                                                                                                                       >  2:26812/1‑249 (MQ=255)                                                                                                                  tatGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATACGGGATACACTGCAACATTAAAGCATGATCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATTTCTTTCATCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTAccc                                                                                                                     >  2:96685/1‑249 (MQ=255)                                                                                                                      ttAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCGACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAAcg                                                                                                                 <  2:42689/250‑2 (MQ=255)                                                                                                                           tttATAATAATGGTTTATTAAGCTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACCGTACCAAACTCATCTg                                                                                                           >  2:52680/1‑251 (MQ=255)                                                                                                                             tataATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATAAACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACAGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTATTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACAGTGCGCGTTTATTAAAACAGTACCAAAATCATCTGa                                                                                                          >  1:1588/1‑250 (MQ=255)                                                                                                                                aataatGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaaa                                                                                                       >  1:8971/1‑250 (MQ=255)                                                                                                                                  taatGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGc                                                                                                    >  2:53864/1‑251 (MQ=255)                                                                                                                                    atGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGc                                                                                                    >  1:247973/1‑249 (MQ=255)                                                                                                                                           attaAGTTTGTTAAGAAACGATCCGGGATACCCTGCAACATTAAAGCATGCCCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGc                                                                                            <  2:337003/250‑1 (MQ=255)                                                                                                                                           ataaaGTTTGTTCAAAAACGATCCGGGATACACGGAAACATTAAAGCATGCCCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTGGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGc                                                                                            <  2:114521/247‑1 (MQ=255)                                                                                                                                               aGTTTTTTCAGAAGCGATCAGGGATACACAGCAACATTAAAGCATGAACAGCCAGTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCGGACAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTAATCTGAAAAAGCAGGCCAGCTatat                                                                                       >  2:47503/1‑251 (MQ=255)                                                                                                                                               aGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTatat                                                                                       <  1:12646/251‑1 (MQ=255)                                                                                                                                                  ttGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATtgt                                                                                    >  1:340869/1‑251 (MQ=255)                                                                                                                                                     ttCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTgg                                                                                  >  2:128962/1‑250 (MQ=255)                                                                                                                                                         gAAACGGTCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCACAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAgg                                                                              >  2:74439/1‑250 (MQ=255)                                                                                                                                                         gAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGc                                                                             >  2:85424/1‑251 (MQ=255)                                                                                                                                                          aaaCGATCCGGGATACACTGCAACATTCAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGc                                                                             >  1:138782/1‑250 (MQ=255)                                                                                                                                                          aaaCGATACGGGATACACTGCAAACTTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGACGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGACGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGCAGGc                                                                             >  2:350116/1‑250 (MQ=255)                                                                                                                                                            aCGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAAt                                                                          >  1:225028/1‑251 (MQ=255)                                                                                                                                                              gATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGc                                                                        >  1:118538/1‑251 (MQ=255)                                                                                                                                                              gATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGc                                                                        >  1:6811/1‑251 (MQ=255)                                                                                                                                                                tCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGccc                                                                      >  2:10656/1‑250 (MQ=255)                                                                                                                                                                cccGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCAGCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTTAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCg                                                                      <  1:53621/250‑1 (MQ=255)                                                                                                                                                                    ggATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGaa                                                                    >  2:221712/1‑249 (MQ=255)                                                                                                                                                                      aTACCCTGCAACATAAAACCATGCCCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCa                                                                <  1:275787/251‑1 (MQ=255)                                                                                                                                                                      aTACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGc                                                                 >  1:238521/1‑250 (MQ=255)                                                                                                                                                                          acTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAACCTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGCAAGCaaa                                                              >  2:311202/1‑249 (MQ=255)                                                                                                                                                                                   ttAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACg                                                   >  1:358298/1‑251 (MQ=255)                                                                                                                                                                                           agcccAGCAATTATTGATTATGTCCTTGCCGTCGATTCCATCCTTTGT‑TTGTTCTGGATTACTGTTGATTTGTTGCCCGTTTTCTTCATGTGCCAGCGCCCCCACATCACATTTCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGcaca                                           <  2:219231/248‑1 (MQ=255)                                                                                                                                                                                            gACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGcaca                                           >  1:118329/1‑250 (MQ=255)                                                                                                                                                                                             aCCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAAc                                         <  2:345039/251‑1 (MQ=255)                                                                                                                                                                                             aCCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAAc                                         >  1:280969/1‑251 (MQ=255)                                                                                                                                                                                              ccAGCCATTATTGAGTATGACCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTTTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCCCATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAAcc                                        <  1:211714/251‑1 (MQ=255)                                                                                                                                                                                                 gCCATTATTGAGCATGTCCTCGCCCTCGATTCCATACATTGTATTGT‑ATGGAATAATGTTGATTTGTTTGCCGTTATCTTCATTTACCAGCGGCACCACATCACCTATCTCTTTCAGCTACGCCGCCCCCCCGCGGATGCAAAACCGTGCGCGTTTATTAAAAAAGTACCAAACTCATCTGAAAAAGCAGGCCCGATATATTGTGCAAGGCAATGCCGAAACCAAAAGGGCGCTACGCCAGAACAACCggg                                     >  2:353692/1‑251 (MQ=255)                                                                                                                                                                                                    attattGAGTATGTCCTTGCCGTCGCTTCCATCCTTTGTATTGT‑CTGGATTACTTTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACTCCGCCCGCCAGCGGCTGCAAAACCGTGCGCGTTTATTAAAACAGTCCCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACACCCGGGAGc                                  >  2:294855/1‑251 (MQ=255)                                                                                                                                                                                                       attGAGAATGTCATTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACCGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTCCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACCCATCGGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAATGCAAAAGGGCGCTACGCCAGCACAACCGGGATCAg                                <  2:176717/250‑1 (MQ=255)                                                                                                                                                                                                         ggAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCTTTTTCTTCATTTGCCAGCGCCACCCCATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGAt                              <  2:345243/249‑1 (MQ=255)                                                                                                                                                                                                                      gccgccGATTCCACCCTTTGTATTTT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCGGCAACGCCGCCCGCCAGCGGATTCAAAACCGt                                                                                                                                            <  1:79487/127‑1 (MQ=255)                                                                                                                                                                                                                      gCCGTCGATTCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGt                                                                                                                                            >  2:79487/1‑127 (MQ=255)                                                                                                                                                                                                                               tCCATCCTTTGTATTGT‑CTTGAGTACTGTTTATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTGAGCTAGGCCGCCCGCCAGCGGATGCAAAACCGTGCGTGTTTATGAAAACTGTTCCATACTCATCTGAAATAGTAGGCGAGTTATATTGTGGGAGGCAATGCCGAAAGCAGAATGGCGCTGCGCTGTCACTATCGGGAGCAGATAAAACAGCATCCAGAATGGttt        <  1:213226/250‑1 (MQ=255)                                                                                                                                                                                                                               tCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGTTGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTc       >  1:255313/1‑251 (MQ=255)                                                                                                                                                                                                                               tCCATCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGttt        <  1:85424/250‑1 (MQ=255)                                                                                                                                                                                                                                  aTCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTg    <  1:158026/251‑1 (MQ=255)                                                                                                                                                                                                                                   tCCTTTGTATTGT‑CTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGc   >  1:135513/1‑251 (MQ=255)                                                                                                                                                                                                                                    ccTTTGTATTGT‑CTGGATTACTTTGGATTTGTGTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCt  <  2:112836/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              GTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGT‑CGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCT  >  NC_000913/246507‑246984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑