Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	92,687	A→G	P425P (CCA→CCG)	ftsI →	peptidoglycan DD‑transpeptidase FtsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	92,687	0	A	G	100.0%	104.4 / NA	29	P425P (CCA→CCG)	ftsI	peptidoglycan DD‑transpeptidase FtsI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/15);  total (14/15)

GGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCT  >  NC_000913/92444‑92931                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     ggCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGt                                                                                                                                                                                                                                               <  2:207416/251‑1 (MQ=255)   cACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGta                                                                                                                                                                                                                                              >  1:164836/1‑249 (MQ=255)                             ggggTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGc                                                                                                                                                                                                                     >  1:182252/1‑249 (MQ=255)                                     aCAGAAGTCGAGTATCGTCGGTGTTTCAAAGCTGGCGTTAGCGATGCCGTCCTCAACGTTAGTAGTTACTTACTCACGCTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCACAAACAACGGTGGTCTGACATACAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGc                                                                                                                                                                                                            >  2:236434/1‑250 (MQ=255)                                        gAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATTGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTCCGGCAttt                                                                                                                                                                                                        >  2:292709/1‑251 (MQ=255)                                              gAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGcc                                                                                                                                                                                                  <  2:79926/251‑1 (MQ=255)                                                       cGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGa                                                                                                                                                                                         <  1:147825/251‑1 (MQ=255)                                                                cAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTCCCAAAg                                                                                                                                                                                <  2:129718/251‑1 (MQ=255)                                                                           tAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGAcccccc                                                                                                                                                                      <  2:296448/250‑1 (MQ=255)                                                                            aGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCg                                                                                                                                                                     <  1:262407/250‑1 (MQ=255)                                                                                          cAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACTGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGAACCCACGGTTCCCGGTGAACgt                                                                                                                                                      >  1:199872/1‑251 (MQ=255)                                                                                                 agtagATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCAGGTTCCCGGTGAACGTGTCTTcc                                                                                                                                               >  2:50662/1‑251 (MQ=255)                                                                                                                                                              cGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGggcggcgg                                                                                  >  1:51021/1‑251 (MQ=255)                                                                                                                                                                                   aaaCAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTaa                                                             <  1:67096/251‑1 (MQ=255)                                                                                                                                                                                             ggtCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgt                                                   <  2:316195/251‑1 (MQ=255)                                                                                                                                                                                                      tAGAGAGGGCCACATTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGTGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCAtt                                          >  1:348806/1‑251 (MQ=255)                                                                                                                                                                                                       agagagGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCAtt                                          >  2:67853/1‑250 (MQ=255)                                                                                                                                                                                                          gagGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTaaaa                                      <  2:87909/251‑1 (MQ=255)                                                                                                                                                                                                           agGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAAc                                     >  1:53676/1‑251 (MQ=255)                                                                                                                                                                                                            gggCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATACGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAAcc                                    <  2:182252/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCAGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCg                 <  2:244687/249‑1 (MQ=255)                                                                                                                                                                                                                                   gggCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGcc             <  1:50662/251‑1 (MQ=255)                                                                                                                                                                                                                                     gCTAATGGTAACAACGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCCGCTACGGCATTTATCGCCCCCTGTCGATTACCAAAGTTGACCCCCCGGTTCCAGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGCTTACAGGCTATCGTATCGCCCTTAAAACCGGTACCGCGAAAAAGGTCGGGCCgg           >  1:54088/1‑251 (MQ=255)                                                                                                                                                                                                                                        aaTGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTGTCGTATCGCCATTAAAGCCGGTACCGCGAAAAAGGGCGGTCCGGACg        <  2:220213/251‑1 (MQ=255)                                                                                                                                                                                                                                          tGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGAAGCTACGGCATTTATCGCCCACTGCCGATTACCAAAGTTGAACCCCCGGCTCCCGGTGAACGTGTCTTACCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGACGGCGGCGATTGACGGCTATCGTAGCGCCATTAAAACCGGTACAGCGACCAAGGTCGTGCCGGACggg      >  2:352090/1‑250 (MQ=255)                                                                                                                                                                                                                                           ggTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTc     <  1:227781/251‑1 (MQ=255)                                                                                                                                                                                                                                             tAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGc   >  1:254541/1‑251 (MQ=255)                                                                                                                                                                                                                                              aaCACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGc   <  2:229821/250‑1 (MQ=255)                                                                                                                                                                                                                                              aaCACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGACGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCt  >  1:13986/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     GGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCT  >  NC_000913/92444‑92931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑